Antiosteoporotic Activity of Anthraquinones from Morinda officinalis on Osteoblasts and Osteoclasts

Abstract

Bioactivity-guided fractionation led to the successful isolation of antiosteoporotic components, i.e. physicion (1), rubiadin-1-methyl ether (2), 2-hydroxy-1-methoxy- anthraquinone (3), 1,2-dihydroxy-3-methylanthraquinone (4), 1,3,8-trihydroxy-2-methoxy- anthraquinone (5), 2-hydroxymethyl-3-hydroxyanthraquinone (6), 2-methoxyanthraquinone (7) and scopoletin (8) from an ethanolic extract of the roots of Morinda officinalis. Compounds 4-8 are isolated for the first time from M. officinalis. Among them, compounds 2 and 3 promoted osteoblast proliferation, while compounds 4, 5 increased osteoblast ALP activity. All of the isolated compounds inhibited osteoclast TRAP activity and bone resorption, and the inhibitory effects on osteoclastic bone resorption of compounds 1 and 5 were stronger than that of other compounds. Taken together, antiosteoporotic activity of M. officinalis and its anthraquinones suggest therapeutic potential against osteoporosis.

Chemical Constituents and Insecticidal Activity of <i>Senna italica</i> Mill. from the Sudan

“…Compounds 1, 2 and 3 were identified as physcion [12], chrysophanol [13] and 10,10′-chrysophanol bianthrone [14] respectively by comparing their TLC chromatograms, EI-MS, 1 H NMR and 13 C NMR spectra with authentic samples and published data. The fragment ions at m/z 270 and 269 correspond to physcion or isophyscion-9-anthrone and those at 241 and 240 belong to chrysophanol-9-anthrone.…”

Identification and Qualitative Analysis of β-Sitosterol and Some Phytoestrogens in In Vivo and In-Vitro Samples of Lepidium sativum: A Semi-Arid Bone Healing Plant

“…Although originally identified in cementum, PTPLa/ CAP is very effective at inducing bone repair and healing and therefore this novel molecule has a great potential to be used for mineralized tissue bioengineering and tissue regeneration studied by [20]. Lepidium sativum seeds have been used in traditional folk medicine to heal fractured bones especially in cases of glucocorticoilds induced osteoporosis [21,22]. Results thus obtained provided a chromatographic fingerprint of specific phytochemicals and are suitable for identify and purify raw materials from such medicinal plants.…”

Health Benefits of Morinda citrifolia (Noni): A Review

“…Finally they suggested all the components have positive therapeutic effect against osteoporosis. 77 Pharmacognosy Journal, Vol 8, Issue 4, Jul-Aug, 2016…”