Antiosteoporotic activity of echinacoside in ovariectomized rats

Li, Fēi; Yang, Xiaolin; Yang, Yanan; Guo, Chengjun; Chunfeng, Zhang; Yang, Zhonglin; Li, Ping

doi:10.1016/j.phymed.2013.01.001

Cited by 82 publications

(53 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Estrogen deficiency is an important risk factor in the pathogenesis of osteoporosis (Li et al, 2013). The PXY is one of the main iso-flavones in radix of P. lobata as puerarin, and the structure of PXY is similar to the soy isoflavone which was reported to be beneficial for women's postmenopausal osteoporosis (Chin & Ima-Nirwana, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The osteoporotic mice model induced by ovariectomy is one of the most common and reliable used models for studying osteoporosis, and this model can simulate commendably the clinical syndromes of women's postmenopausal osteoporosis. Furthermore, the phenomenon of menopause is a signal of ovarian failure, which also means reduction of estrogen, resulting in bone loss increasingly (Kalu, 1991;Li et al, 2013). In previous investigations, it is reported that ovariectomy can lead to a significantly increase in body weight and decrease in uterus' weights, and these changes partly due to estrogen deficiency (Li et al, 2013;Nian et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, the phenomenon of menopause is a signal of ovarian failure, which also means reduction of estrogen, resulting in bone loss increasingly (Kalu, 1991;Li et al, 2013). In previous investigations, it is reported that ovariectomy can lead to a significantly increase in body weight and decrease in uterus' weights, and these changes partly due to estrogen deficiency (Li et al, 2013;Nian et al, 2009). The results of our present study demonstrated that OVX decreased the uterine index and increased the body weight compared with the Sham group, which clearly showed the successful establishment of osteoporotic mice model.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Anti-osteoporotic activity of puerarin 6″-O-xyloside on ovariectomized mice and its potential mechanism

Li¹,

Bing-pu²,

Pang

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Osteoporosis is one of the most common bone diseases, and radix of Pueraria lobata (Willd.) Ohwi possesses an obvious therapeutical effect on postmenopausal osteoporosis. Objective: This study investigates the anti-osteoporotic activity of the puerarin 6 00 -O-xyloside (PXY) on ovariectomized mice and its related mechanism. Materials and methods: Osteoporotic mice model was established by ovariectomy (OVX). A total of 50 mice were divided into five groups (n ¼ 10): sham, OVX group, PXY treatment groups (20, 40, and 60 mg/kg/d, i.p.). After 12 weeks' treatment, body weights were recorded. Then, mice were sacrificed, and serum samples were collected to determine the blood calcium, blood phosphorus, alkaline phosphatase (ALP), and osteoprotegerin (OPG) concentrations and uterine index was assayed. The thigh-bones of mice were collected to evaluate histopathological changes. In the in vitro experiment, the effect of PXY on osteoblasts' proliferation was evaluated and western blotting was performed to determine expressions of OPG and the receptor activators of NF-B ligand (RANKL), as well as the ratio of OPG/RANKL. Results: PXY (40 and 60 mg/kg/d, i.p.) obviously decreased body weights and increased uterine index of OVX (p50.05), and improved osteoporotic syndromes of OVX mice; PXY also significantly increased the concentrations of blood calcium, blood phosphorus, ALP, and OPG of OVX mice (p50.05); moreover, PXY obviously up-regulated the ratio of OPG/RANKL (p50.05). Conclusion: Our results demonstrated that the puerarin 6 00 -O-xyloside possesses significant anti-osteoporotic activity on ovariectomy mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-osteoporotic activity of puerarin 6″-O-xyloside on ovariectomized mice and its potential mechanism

Li¹,

Bing-pu²,

Pang

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

show abstract

“…It is well known that serum estradiol (E2) level is the widely accepted phenotype marker for bone metabolism. Hence, serum E2 level can also be determined using enzyme-linked immunosorbent assay (ELISA) kit following manufacturer's instruction [50].…”

Section: Determination Of Protein Profile and Serum Estradiolmentioning

confidence: 99%

“…Selected uteri are fixed in 10% formalin for 2 days at room temperature, then tissues are washed, dehydrated in gradient alcohol, embedded in paraffin wax and cut into serial sagittal sections (4 µm thick) with a microtome. Immunohistochemical localization of ER is done with ER polyclonal antibody according to the manufacturer's instructions [50].…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

Satpathy

Patra

Ahirwar

2015

Journal of Complementary and Integrative Medicine

View full text Add to dashboard Cite

Postmenopausal osteoporosis, a silent epidemic, has become a major health hazard, afflicting about 50% of postmenopausal women worldwide and is thought to be a disease with one of the highest incidences in senile people. It is a chronic, progressive condition associated with micro-architectural deterioration of bone tissue that results in low bone mass, decreased bone strength that predisposes to an increased risk of fracture. Women are more likely to develop osteoporosis than men due to reduction in estrogen during menopause which leads to decline in bone formation and increase in bone resorption activity. Estrogen is able to suppress the production of proinflammatory cytokines like interleukin (IL)-1, IL-6, IL-7 and tumor necrosis factor (TNF-α). This is why these cytokines are elevated in postmenopausal women. In this review article we have made an attempt to collate the various methods and parameters most frequently used for screening of antiosteoporotic activity in postmenopausal osteoporosis. Pertaining to ovariectomized animal model, this is the most appropriate model for studying the efficacy of different drugs to prevent bone loss in postmenopausal osteoporosis.

show abstract

Isoflavone‐enriched soybean leaves attenuate ovariectomy‐induced osteoporosis in rats by anti‐inflammatory activity

et al. 2020

View full text Add to dashboard Cite

BACKGROUND With an increasing aging population, postmenopausal osteoporosis has become a global public health problem. Previous evidence has shown that postmenopausal osteoporosis is a skeletal disease mainly caused by estrogen deficiency, generally accompanied by inflammation, and dietary isoflavones may ameliorate postmenopausal osteoporosis by anti‐inflammatory activity. We have generated isoflavone‐enriched soybean leaves (IESLs), but their anti‐inflammatory activity and effect on attenuating osteoporosis are still obscure. Here, we determined the isoflavone profiles of IESLs and evaluated their anti‐inflammatory activity in lipopolysaccharide‐stimulated RAW 264.7 cells and anti‐osteoporotic effects on ovariectomy‐induced osteoporosis in rats. RESULTS IESLs had a high content of total isoflavone. Hydrolysate of IESLs (HIESLs) was rich with the aglycones daidzein and genistein, and HIESLs can significantly inhibit lipopolysaccharide‐induced inflammation by reducing messenger RNA expression of iNOS, COX‐2, IL6, and IL1β. Moreover, ovariectomized rats receiving aqueous extracts of IESLs (HIESLs) orally maintained more bone mass than control rats did, which was attributed to inhibition of osteoclastogenesis by downregulating the messenger RNA expression of the bone‐specific genes RANKL/OPG, OC, and cathepsin K, and the inflammation‐related genes IL6, NFκB, and COX‐2. CONCLUSION IESLs may attenuate postmenopausal osteoporosis by suppressing osteoclastogenesis with anti‐inflammatory activity and be a potential source of functional food ingredients for the prevention of osteoporosis. © 2020 Society of Chemical Industry

show abstract

Antiosteoporotic activity of echinacoside in ovariectomized rats

Cited by 82 publications

References 23 publications

Anti-osteoporotic activity of puerarin 6″-O-xyloside on ovariectomized mice and its potential mechanism

Anti-osteoporotic activity of puerarin 6″-O-xyloside on ovariectomized mice and its potential mechanism

Experimental techniques for screening of antiosteoporotic activity in postmenopausal osteoporosis

Isoflavone‐enriched soybean leaves attenuate ovariectomy‐induced osteoporosis in rats by anti‐inflammatory activity

Contact Info

Product

Resources

About