Antiosteoporotic Activity of the Water Extract of Dioscorea spongiosa

Abstract

After 60 MeOH and water extracts of natural crude drugs were screened for their ability to stimulate osteoblast proliferation, four MeOH extracts (Cynomorium songaricum, Drynaria fortunei, Lycium chinense, Rehmannia glutinosa) and seven water extracts (Cornus officinalis, Dendrobium nobile, Dioscorea spongiosa, Drynaria fortunei, Eucommia ulmoides, Lycium chinensis, Viscum coloratum) showed that potent activities were evaluated for inhibition of osteoclast formation. The results indicated that the water extract of D. spongiosa not only showed the strongest stimulation of osteoblast proliferation but also possessed potent inhibitory activity aganist osteoclast formation, whereas it showed lower cytotoxicity in osteoblast and bone marrow cells. A further in vivo experiment determined the antiosteoporotic activity of this extract, in which it inhibited the decrease in cancellous bone mineral content, cancellous bone mineral density, and cortical bone mineral content of the proximal tibia in ovariectomized rats.

Diosgenin inhibits osteoclastogenesis, invasion, and proliferation through the downregulation of Akt, IκB kinase activation and NF-κB-regulated gene expression

“…We demonstrate for the first time that diosgenin inhibited RANKL-induced osteoclastogenesis by blocking the differentiation of monocytes into osteoclasts. This result may explain the antiosteoporotic effects of diosgenin reported previously Scott et al, 2001;Yin et al, 2004). We also show that diosgenin suppressed TNF-induced invasion by tumor cells, and this inhibition correlated with the downregulation of MMP-9 and COX-2.…”

DA-9801 Promotes Neurite Outgrowth <i>via</i> ERK1/2-CREB Pathway in PC12 Cells

“…17,18) The extracts of plants from the Dioscorea genus were reported to exhibit hypoglycemic, 19) immunostimulatory, 20) and anti-inflammatory effects, 21) as well as anti-tumor 22) and anti-osteoporotic activity. 23) Dioscorea japonica THUNBERG. (DJ) has been utilized in the treatment of hyperglycemia in Korea.…”

Induction of apoptosis byDioscorea nipponicaMakino extracts in human SH-SY5Y neuroblastoma cells via mitochondria-mediated pathway

“…Traditionally, many species of Dioscorea have been used as folk medicine for syndromes related to metabolic disorders. The extracts of Dioscorea species have been reported to have a hypoglycemic effect (Hikino et al 1986), immunostimulatory effect (Zhao et al 2005), antiinflammatory effects (Kim et al 2004), antitumor activity (Hu & Yao 2003), and antiosteoporotic activity (Yin et al 2004). D. nipponica Makino has been used to cure coronary heart disease in China and its methanol extract has shown a potential antiobesity effect (Lin et al 2007).…”