Antioxidant activities and selected characteristics of gelatin hydrolysates from seabass (Lates calcarifer) skin as affected by production processes

Abstract

Antioxidant activities and selected characteristics of gelatin hydrolysates from seabass skin as affected by production processes were investigated. Hydrolysates were prepared using different processes, including hydrolysis during and after gelatin extraction. Samples hydrolysed during gelatin extraction showed a higher degree of hydrolysis (DH) and yield compared with those hydrolysed after gelatin extraction (p<0.05). All hydrolysates had a creamy yellowish colour. A lower abundance of volatile compounds was found in the hydrolysates produced during gelatin extraction, in comparison with those obtained after gelatin extraction. Hydrolysates prepared during gelatin extraction had higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidative power (FRAP) and ferrous ion chelating activity (p<0.05). Following a simulated in vitro gastrointestinal digestion, the DPPH radical scavenging activity and FRAP of the hydrolysates was retained, whilst ferrous ion chelating activity increased. The most appropriate conditions for the generation of antioxidant hydrolysates from seabass skin were identified.

Antioxidant activities of hydrolysed collagen from salmon scale ossein prepared with the aid of ultrasound

“…The simulated in vitro gastrointestinal digestion was performed according to the method of Sae‐leaw et al . (2016b). Hydrolysed collagen powder was dissolved in distilled water to obtain a concentration of 0.5 g mL −1 .…”

“…Oxygen radical absorbance capacity (ORAC) of undigested hydrolysed collagen powder and those obtained after gastric and intestinal digestion was also determined as per the method of Sae‐leaw et al . (2016b). The samples were dissolved in 75 m m phosphate buffer (pH 7.0) to obtain a final concentration of 0.1 mg mL −1 .…”

Antioxidant, immunomodulatory and antiproliferative effects of gelatin hydrolysates from seabass (Lates calcarifer) skins

“…Such activities lead to the inhibition of the Fenton reaction and, therefore, protected the DNA from oxidant‐induced strand breaks (Je et al ., ). From the result of previous study, sample 1 had a higher Fe 2+ chelating ability (Sae‐leaw et al ., ) than the remaining samples which could account for its greater protective effect against oxidant‐induced DNA damage. Seabass skin hydrolysates prepared with different processes showed various protective effects against DNA damage which may be due to the differences in their chain length, amino acid compositions and sequences.…”

“…The skins were pretreated using 0.05 m NaOH and swollen using 0.05 m citric acid as described by Sae‐leaw et al . ().…”

Effect of drying method on functional properties and antioxidant activities of chicken skin gelatin hydrolysate

“…This finding shows that chicken skin gelatin contains a low molecular weight compared to bovine gelatin and possesses better antioxidant activity. Besides, the study reported by Sae-leaw et al (2016) reported that the gelatin hydrolysate that prepared after the gelatin extraction resulted a lower DPPH scavenging activities due to the structure of the peptides of hydrolysate as compared to the gelatin hydrolysate that prepared during the gelatin extraction.…”