Antioxidant Activities of Aster glehni Extracted with Different Solvents

Seo, Sangwook; Kim, Kisok

doi:10.18502/ijph.v48i1.808

Cited by 1 publication

(1 citation statement)

References 7 publications

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“…Selecting the appropriate extraction technology and optimizing relevant process parameters can greatly reduce the time and economic costs of extraction processes [11]. There are many methods for extracting the active ingredients of natural medicines, including solvent extraction (SE) [12], steam distillation (SD) [13], ultrasonic-assisted extraction (UAE) [14], microwave-assisted extraction (MAE) [15], supercritical-fluid extraction (SFE) [16], enzyme hydrolysis (EH) [17], and other methods [18]. Among them, UAE has the advantages of simple extraction, convenient operation, high extraction efficiency, and short extraction time, making it the most commonly used process [19].…”

Section: Introductionmentioning

confidence: 99%

Response Surface Modelling of Six Organic Acids from Pinellia ternata (Thunb.) Breit by Ultrasound-Assisted Extraction and Its Determination by High-Performance Liquid Chromatography Coupled to Triple Quadrupole Mass Spectrometry

Wei,

Li,

Zhang

et al. 2024

Chemosensors

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Organic acids are a key active component of Pinellia ternata (Thunb.) Breit, and their concentration is closely associated with the quality of P. ternata. Developing an efficient and rapid method for detecting organic acids can offer a valuable technology for real-time assessment of P. ternata quality. In this work, a high-performance liquid chromatography coupled to triple quadrupole mass spectrometry (HPLC-QqQ-MS/MS) approach, combining the optimization of extraction conditions using response surface methodology (RSM), was developed for the simultaneous quantitative analysis of six organic acids in P. ternata. The optimal extraction conditions for organic acids in P. ternata were ultrasonic extraction with a solid–liquid ratio of 1:50, ultrasonic time of 60 min, and extraction temperature of 55 °C. Multiple-reaction monitoring (MRM) scanning was employed for quantification using HPLC-QqQ-MS/MS in negative ionization mode through a single run of 10 min, and the limit of detection (LLOD) and limit of quantification (LLOQ) for organic acids were as low as 0.138 ng/mL and 0.614 ng/mL, respectively. The relative standard deviations (RSDs) of reproducibility, precision, and stability were all lower than 5.0%, and recovery rates were 97.75–107.14%, with RSDs < 5.0%. Finally, this method was successfully applied to determine the investigated organic acids in 12 production regions of P. ternata, revealing significant differences between different production areas. This indicates that the optimized method is suitable for further accurate investigations of organic acids of P. ternata.

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