Antioxidant activities of carnosine, anserine, some free amino acids and their combination

Wu, Hui-Chun; Shiau, Chyuan‐Yuan; Chen, Hua‐Ming; Chiou, Tze‐Kuei

doi:10.38212/2224-6614.2720

Cited by 53 publications

(54 citation statements)

References 20 publications

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“…The absorbance was measured at 485 nm and 520 nm to monitor the murexide-copper complex, and free murexide in solution, respectively. The absorbance ratio A 485 /A 520 was considered to be proportional to the amount of free Cu(II) concentration in solution [56,57].…”

Section: Copper(ii)-chelating Activitymentioning

confidence: 99%

“…In a first set of experiments, we evaluated the Fe(II) and the Cu(II) chelating ability of GQDs and compared the results with those that were obtained while using EDTA and carnosine, compounds that are well-known to exhibit metal chelating properties [53][54][55][56][57]. GQDs, used at various concentrations, were added to a 2 mM solution and FeCl 2 and the mixture was incubated for 3 min at room temperature.…”

Section: Fe(ii) and Cu(ii) Chelating Propertiesmentioning

confidence: 99%

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One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

et al. 2020

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2,2’-(Ethylenedioxy)bis(ethylamine)-functionalized graphene quantum dots (GQDs) were prepared under mild conditions from graphene oxide (GO) via oxidative fragmentation. The as-prepared GQDs have an average diameter of ca. 4 nm, possess good colloidal stability, and emit strong green-yellow light with a photoluminescence (PL) quantum yield of 22% upon excitation at 375 nm. We also demonstrated that the GQDs exhibit high photostability and the PL intensity is poorly affected while tuning the pH from 1 to 8. Finally, GQDs can be used to chelate Fe(II) and Cu(II) cations, scavenge radicals, and reduce Fe(III) into Fe(II). These chelating and reducing properties that associate to the low cytotoxicity of GQDs show that these nanoparticles are of high interest as antioxidants for health applications.

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Section: Copper(ii)-chelating Activitymentioning

confidence: 99%

Section: Fe(ii) and Cu(ii) Chelating Propertiesmentioning

confidence: 99%

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

et al. 2020

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“…Previous studies have focused on chicken essence when attributing anserine and carnosine's various antioxidative, antiglycating, anti-inflammation, antilipogenic, and antifatigue protective properties [3][4][5][6][7]. Others have only focused on carnosine's antioxidative role [8].…”

Section: Introductionmentioning

confidence: 99%

Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men

et al. 2020

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The study tested whether anserine (beta-alanyl-3-methyl-l-histidine), the active ingredient of chicken essence affects exercise-induced oxidative stress, cell integrity, and haematology biomarkers. In a randomized placebo-controlled repeated-measures design, ten healthy men ingested anserine in either a low dose (ANS-LD) 15 mg·kg−1·bw−1, high dose (ANS-HD) 30 mg·kg−1·bw−1, or placebo (PLA), following an exercise challenge (time to exhaustion), on three separate occasions. Anserine supplementation increased superoxide dismutase (SOD) by 50% (p < 0.001, effect size d = 0.8 for both ANS-LD and ANS-HD), and preserved catalase (CAT) activity suggesting an improved antioxidant activity. However, both ANS-LD and ANS-HD elevated glutathione disulfide (GSSG), (both p < 0.001, main treatment effect), and consequently lowered the glutathione to glutathione disulfide (GSH/GSSG) ratio compared with PLA (p < 0.01, main treatment effect), without significant effects on thiobarbituric acid active reactive substances (TBARS). Exercise-induced cell damage biomarkers of glutamic-oxaloacetic transaminase (GOT) and myoglobin were unaffected by anserine. There were slight but significant elevations in glutamate pyruvate transaminase (GPT) and creatine kinase isoenzyme (CKMB), especially in ANS-HD (p < 0.05) compared with ANS-LD or PLA. Haematological biomarkers were largely unaffected by anserine, its dose, and without interaction with post exercise time-course. However, compared with ANS-LD and PLA, ANS-HD increased the mean cell volume (MCV), and decreased the mean corpuscular haemoglobin concentration (MCHC) (p < 0.001). Anserine preserves cellular homoeostasis through enhanced antioxidant activity and protects cell integrity in healthy men, which is important for chronic disease prevention. However, anserine temporal elevated exercise-induced cell-damage, together with enhanced antioxidant activity and haematological responses suggest an augmented exercise-induced adaptative response and recovery.

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“…Despite EP‐digested caseins showed less DH than EM‐digested caseins, we found that the antioxidant activity of EP‐digested caseins was higher in all considered times than that obtained with the EM extract ( P < 0.05). Taking into account that the antioxidant activity during hydrolysis is affected by enzyme specificity that generates peptides and free amino acids with different size, level and composition (Wu et al , ), the explanation could be ascribed to the pepsin action, specific towards hydrophobic amino acids which, following cleavage, become exposed facilitating the transfer of electrons to the reactive oxygen species (Power et al , ). EM extract, on the other hand, is characterised by the presence of proteolytic enzymes with different cleavage sites which act simultaneously on the substrate, causing an increase in the DH over time, but also the probable release of low antioxidant capacity peptides and inactive peptides.…”

Section: Resultsmentioning

confidence: 99%

Hydrolytic degree and antioxidant activity of purified casein characterised by different haplotypes (α_s1‐, β‐ and k‐casein) after enzymatic hydrolysis with pepsin and enzymatic extract from Pleurotus eryngii

Perna

Simonetti

Acquaviva

et al. 2019

Int J of Food Sci Tech

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The aim of this study was to estimate the hydrolytic degree and antioxidant activity of purified casein characterised by different haplotypes (a s1 -, band k-casein) after in vitro digestion with two different enzymatic systems: pepsin from porcine gastric mucosa (EP) and a crude enzymatic extract from the edible mushroom Pleurotus eryngii. The used enzymes showed a different mode of casein catalysis with a consequent production of peptides of different antioxidant activity. The CN haplotype significantly influenced peptides production; in fact, the amino acid substitutions caused by genetic polymorphisms at the a s1 -, band k-CN loci influenced the sites of enzymatic cleavage and therefore the produced peptides. The above is evidenced by the different antioxidant activity found in the hydrolysates depending on the used enzymatic system, the CN haplotype, and the CN haplotype 9 enzymatic treatment interaction. The findings of this study are a perspective for the production of specific foods that exert a biological effect in addition to the nutritional one.

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Antioxidant activities of carnosine, anserine, some free amino acids and their combination

Cited by 53 publications

References 20 publications

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men

Hydrolytic degree and antioxidant activity of purified casein characterised by different haplotypes (α_s1‐, β‐ and k‐casein) after enzymatic hydrolysis with pepsin and enzymatic extract from Pleurotus eryngii

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Antioxidant activities of carnosine, anserine, some free amino acids and their combination

Cited by 53 publications

References 20 publications

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

Anserine Reverses Exercise-Induced Oxidative Stress and Preserves Cellular Homeostasis in Healthy Men

Hydrolytic degree and antioxidant activity of purified casein characterised by different haplotypes (αs1‐, β‐ and k‐casein) after enzymatic hydrolysis with pepsin and enzymatic extract from Pleurotus eryngii

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Product

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About

Hydrolytic degree and antioxidant activity of purified casein characterised by different haplotypes (α_s1‐, β‐ and k‐casein) after enzymatic hydrolysis with pepsin and enzymatic extract from Pleurotus eryngii