Antioxidant activities of chitosans and its derivatives in in vitro and in vivo studies

Anraku, Makoto; Gebicki, Janusz M.; Iohara, Daisuke; Tomida, Hisao; Uekama, Kaneto; Maruyama, Toru; Hirayama, Fumitoshi; Otagiri, Masaki

doi:10.1016/j.carbpol.2018.07.016

Cited by 133 publications

(63 citation statements)

References 85 publications

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“…The Alamar Blue test is a very popular method, and over the last 50 years it has been widely used in cell viability and cytotoxicity studies due to its speed, sensitivity, and reduced cost(Damiani, Solorio, Doyle, & Wallace, 2019; Tahara et al, 2017). The healing effect of chitosan and flavonoid has been attributed to the antimicrobial, antiinflammatory, and antioxidant properties of these compounds(Anraku et al, 2018). Thus, according to the results obtained, the hydrogels under study can be considered biocompatible materials.…”

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confidence: 98%

Development of a chitosan hydrogel containing flavonoids extracted fromPassiflora edulisleaves and the evaluation of its antioxidant and wound healing properties for the treatment of skin lesions in diabetic mice

Soares

Campos

Ribeiro

et al. 2019

J Biomedical Materials Res

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This study aimed to develop a chitosan-based hydrogel containing a mixture of flavonoids isolated from the leaves of Passiflora edulis Sims and to evaluate its stability, antioxidant properties, and wound healing effects on cutaneous lesions in diabetic rats. in vitro studies were carried out to evaluate the biocompatibility and flavonoid release from the chitosan hydrogel. in vivo wound healing studies were conducted on male Wistar rats, where the injured tissue was removed for histological analysis and determination of lipid peroxidation, superoxide dismutase activity, and glutathione peroxidase activity. From the histological analysis and macroscopic evaluation of the contraction of the wounds, it was observed that the formulation presented wound healing properties. In addition, treatment of the wound with the formulation stimulated the antioxidant defense system, suggesting a beneficial effect during the treatment of skin lesions in diabetic rats, especially in the first few days after wounding. According to these results, we can conclude that the chitosan hydrogel containing the flavonoid analyzed in this study has potential use as dressings in the treatment of wounds. K E Y W O R D S antioxidant, chitosan, flavonoid, hydrogel, Passiflora edulis, wound healing

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mentioning

confidence: 98%

Development of a chitosan hydrogel containing flavonoids extracted fromPassiflora edulisleaves and the evaluation of its antioxidant and wound healing properties for the treatment of skin lesions in diabetic mice

Soares

Campos

Ribeiro

et al. 2019

J Biomedical Materials Res

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“…It could be concluded that αand β-chitosan nanoparticles had superior superoxide radical scavenging abilities than raw chitosan samples.affect their antioxidant activity [7,8]. The antioxidant activities of chitosan have been extensively studied both in vitro and in vivo using different methodologies and are reported to be correlated to its structural characteristics such as degree of deacetylation (%DD), molecular weight (MW), as well as the source of the material [9][10][11]. The physicochemical properties of chitosan end products can be modulated by controlling factors such as chitin source of origin, reaction conditions (concentration, ratios of chitin to alkali, temperature), and extent of the reaction.…”

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confidence: 99%

“…The physicochemical properties of chitosan end products can be modulated by controlling factors such as chitin source of origin, reaction conditions (concentration, ratios of chitin to alkali, temperature), and extent of the reaction. It is known that hydroxyl, and amino groups in chitosan are key components in eliminating anion radicals such as superoxide and hydroxyl radicals [8][9][10]12] It has been reported that the amino and hydroxyl groups on chitosan backbone represent target moieties for chemical modifications to improve the aqueous solubility of chitosan since it is soluble only in an aqueous acidic solution, which is a major hurdle for its application [5]. The unique properties of chitosan, such as nontoxicity to humans [13][14][15]; commercial accessibility, biodegradability and biocompatibility [14,15], and high mucoadhesive properties [15,16] make chitosan an excellent choice for nanoparticle assembly.…”

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confidence: 99%

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

2019

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α- and β-Chitosan nanoparticles were obtained from shrimp shell and squid pen chitosan with different set of deacetylation degree (%DD) and molecular weight (MW) combinations. After nanoparticle formation via ionic gelation with sodium tripolyphosphate (TPP), the % crystallinity index (%CI) of the α- and β-chitosan nanoparticles were reduced to approximately 33% and 43% of the initial %CI of the corresponding α- and β-chitosan raw samples, respectively. Both forms of chitosan and chitosan nanoparticles scavenged superoxide radicals in a dose-dependent manner. The %CI of α- and β-chitosan and chitosan nanoparticles was significantly negatively correlated with superoxide radical scavenging abilities over the range of concentration (0.5, 1, 2 and 3 mg/mL) studied. High %DD, and low MW β-chitosan exhibited the highest superoxide radical scavenging activity (p < 0.05). α- and β-Chitosan nanoparticles prepared from high %DD and low MW chitosan demonstrated the highest abilities to scavenge superoxide radicals at 2.0–3.0 mg/mL (p < 0.05), whereas α-chitosan nanoparticles, with the lowest %CI, and smallest particle size (p < 0.05), prepared from medium %DD, and medium MW chitosan showed the highest abilities to scavenge superoxide radicals at 0.5–1.0 mg/mL (p < 0.05). It could be concluded that α- and β-chitosan nanoparticles had superior superoxide radical scavenging abilities than raw chitosan samples.

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“…Reactive oxygen species (ROS) are by‐products of toxic oxygen metabolism and the products are the result of ionizing radiation or aging of some chemicals. Antioxidants are the substances which inhibit oxidation . Nowadays, searching for environmentally friendly and efficient antioxidants is becoming increasingly urgent.…”

Section: Resultsmentioning

confidence: 99%

Improved Antioxidant and Antifungal Activity of Chitosan Derivatives Bearing Urea Groups

Zhang

Sun

et al. 2020

Starch Stärke

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Eight novel chitosan derivatives bearing urea groups are designed and synthesized. Fourier transform infrared spectroscopy, 1 H nuclear magnetic resonance spectrometer, and elemental analysis are performed to confirm the structural characteristics of chitosan derivatives. The antioxidant activities, including superoxide radicals' scavenging activity, 1,1-diphenyl-2-picrylhydrazyl radicals' scavenging activity, and hydroxyl radical' scavenging activity, of the derivatives are explored within different concentrations in the reaction system. In vitro fungicidal activity of these compounds is further tested against Fusarium oxysporum f. sp. niveum, Phomopsis asparagus, F. oxysporum f. sp. cucumebrium Owen, and Botrytis cinerea, respectively, particularly compounds exhibit significant control effect at 1.0 mg mL −1 . The experimental results indicate that the products bearing urea groups show enhanced antifungal property and antioxidant activity compared with pristine chitosan. Meanwhile, their bioactivities follow some regularity on the whole, that is, they are related to the electron-withdrawing property of the different substituted groups of urea. Derivatives with stronger electron-withdrawing property will have higher biological activities. L929 cells are used to carry out cytotoxicity test of chitosan and chitosan derivatives by Cell Counting Kit-8 assay. The results indicate that some of the samples show low cytotoxicity. This research will be helpful to broaden the application of chitosan in materials.

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Antioxidant activities of chitosans and its derivatives in in vitro and in vivo studies

Cited by 133 publications

References 85 publications

Development of a chitosan hydrogel containing flavonoids extracted fromPassiflora edulisleaves and the evaluation of its antioxidant and wound healing properties for the treatment of skin lesions in diabetic mice

Development of a chitosan hydrogel containing flavonoids extracted fromPassiflora edulisleaves and the evaluation of its antioxidant and wound healing properties for the treatment of skin lesions in diabetic mice

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

Improved Antioxidant and Antifungal Activity of Chitosan Derivatives Bearing Urea Groups

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