Antioxidant Activities of Chokeberry Extracts and the Cytotoxic Action of Their Anthocyanin Fraction on HeLa Human Cervical Tumor Cells

Abstract

The present study evaluates the antioxidant activity of two Aronia melanocarpa cultivars-Viking and Aronand of Aronia prunifolia hybrid in relationship with their phytochemical composition regarding the contents of total phenolics, flavonoids, procyanidins, and monomeric anthocyanins. The antioxidant capacity of the mentioned extracts of chokeberries was evaluated through five complementary assays: 2,2 0 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), H2O2 scavenging potential, oxygen radical absorbance capacity, ferric reducing antioxidant power, and cupric ion reducing antioxidant capacity. A. prunifolia hybrid was found to have the highest antioxidant activity and to be the richest in polyphenols, procyanidins, and anthocyanins compared with the A. melanocarpa cultivars. A good correlation was observed between antioxidant activity and total procyanidin and anthocyanin content. Cyanidin glycosides inhibited HeLa human cervical tumor cell proliferation and increased generation of reactive oxygen species after 48 h of treatment, suggesting that they could be responsible for the antiproliferative activity. These results may be significant for industry concerning food quality and disease prevention.

Phenolic Composition, Radical Scavenging Activity and an Approach for Authentication of Aronia melanocarpa Berries, Juice, and Pomace

“…(), A. melanocarpa contained the highest anthocyanin content (9.06 ± 5.06 µg/g DW) followed by A. prunifolia (3.21 ± 0.65 µg/g DW) and A. arbutifolia (0.63 ± 0.17 µg/g DW), whereas Ruginǎ et al. () described a higher total anthocyanin content in A. prunifolia (366 mg/100 g fresh weight FW) than in A. melanocarpa ‘Viking‘ (277 mg/100 g FW) and ‘Aron‘ (176 mg/100 g FW) cultivars. Moreover, the same trend was observed for total procyanidin content: A. prunifolia (855.13 mg epicatechin/100 g FW), ‘Viking‘ (627.57 mg epicatechin/100 g FW) and ‘Aron‘ (354.94 mg epicatechin/100 g FW).…”

Optimization of polyphenols extraction from dried chokeberry using maceration as traditional technique

“…Fully ripened chokeberry fruits contain different phenolic compounds such as proanthocyanidins, flavan-3-ol and flavonol glycosides, and phenolic acids (Rugina et al, 2012;Suiero et al, 2006). Moreover, chokeberry represents one of the richest plant sources of anthocyanins exhibiting strong antioxidant activity (Braunlich et al, 2013).…”

Antimicrobial and Antioxidant Activities of Bacterial Extracts from Marine Bacteria Associated with Sponge <i>Stylotella</i> sp.

“…Bacteria isolated from sponge Stylotella sp also showed better activity compared to several Chokeberry extracts which also had potential antioxidant and cytotoxic. Aronia prunifolia cultivar had antioxidant activity was 232.30 µmol trolox/g extract, cultivar Viking of A. Melanocarpa was 206.20 µmol trolox/g extract and cultivar Aron of A. Melanocarpa was 177.80 µmol trolox/g extract (Rugina et al, 2012).…”