Antioxidant Activities of Ostrich Fern by Different Extraction Methods and Solvents

Shin, Soo‐Jeong; Lee, Cheol Hee

doi:10.5352/jls.2011.21.1.56

Cited by 31 publications

(7 citation statements)

References 11 publications

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“…Total flavonoid contents of E-RC (65.9 mg/g) were also higher than that of PHWE-RC (37.9 mg/g). Ethanolic extraction was more effective method due to polarity of the compounds and the result was corresponded to previous report [31]. The result indicated rice cell extracts contained comparable level of those compounds to other plant extracts [25].…”

Section: Resultssupporting

confidence: 87%

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Evaluation of the Wound-healing Activity of Rice Cell Extracts in Vitro

Kim¹,

Kim²,

Park³

et al. 2016

Microbiology and Biotechnology Letters

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In the present study, we evaluated the in vitro wound-healing properties of two types of rice cell extracts (RCEs; prepared using ethanol and pressurized hot water extraction methods), using human dermal fibroblasts and keratinocytes. The effects of the RCEs (at 25-100 µg/ml) on cytotoxicity and cell migration were assessed. Both RCEs were not cytotoxic to the two cell types, instead increasing their proliferation by up to 25% in a dose-dependent manner compared with the controls. Furthermore, both RCEs significantly enhanced the migratory ability of the two cell types (fibroblast, 230-450%; keratinocyte, 170-350%). Additionally, we examined the effect of the RCEs on type I collagen synthesis, which is important in the wound reconstruction process. The RCEs significantly increased collagen type I mRNA and protein levels to a degree comparable to that induced by vitamin C. These results suggest the RCEs to be candidate materials for use in promoting wound healing, through their actions of increasing cell migration and accelerating wound re-epithelialization.

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Section: Resultssupporting

confidence: 87%

“…The total flavonoid contents of two rice extracts were determined according to the previous report [31]. Briefly, after 200 µl of 10-fold diluted extracts, 200 µl of 1 N NaOH, and 2 ml of diethyl glycol were mixed each other, the solution was incubated for 1 h at 37℃.…”

Section: Determination Of Total Phenolic and Flavonoid Contentsmentioning

confidence: 99%

Evaluation of the Wound-healing Activity of Rice Cell Extracts in Vitro

Kim¹,

Kim²,

Park³

et al. 2016

Microbiology and Biotechnology Letters

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“…곰취, 들깻잎, 부추 [29], 생강, 들깨, 쑥갓, 방아 등의 식용식 물 [32], 버섯 [18], 도라지 [15], 고사리 [36] 등의 항산화 효과, 무 [5] Stimulation (PMA, A23187), Con: Not treated, A-1~A-3: raw Cucurbita moschata P. (0.01, 0.1 and 1.0 mg/ml, respectively), A1~A3: seasoned Cucurbita moschata P. (0.01, 0.1 and 1.0 mg/ml, respectively), B-1~B-3: raw Platycodon grandiflorum A. (0.01, 0.1 and 1.0 mg/ml, respectively), B1~B3: seasoned Platycodon grandiflorum A.…”

Section: 서 론mentioning

confidence: 99%

Anti-inflammatory Effect and Antioxidative Activities of Ingredients used in Bibimbab

Ko¹,

Seol²,

Lee³

et al. 2013

Journal of Life Science

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Bibimbab (mixed rice) is a traditional Korean one-dish meal. This study was carried out to investigate the anti-inflammatory and antioxidative effects of raw and seasoned ingredients used in Bibimbab (Cucurbita moschata P., Platycodon grandiflorum A., Vigna radiata L., Porphyra yezonensis udea, Allium ampeloprasum L., Pterdium aguilinum, Raphanus sativus). Human mast cells (HMC-1) were pretreated with 70% ethanol extracts of Bibimbab and further cultured for an appropriate time after the addition of phorbol 12-myristate 13-acetate (PMA) and calcium ionophore A23187. Cell viability was determined by an MTT assay. None of the ingredients showed cytotoxic effects at a concentration of 1.0 μg/ml. Anti-inflammatory effects were analyzed at 0.01, 0.1, and 1.0 mg/ml concentrations of the raw, seasoned ingredients of PMA. A23187 stimulated HMC-1. Among the various ingredients, seasoned A. ampeloprasum L. extract showed the highest inhibition of TNF-α and IL-6 secretion (90% and 93%, respectively) at a concentration of 1.0 mg/ml. The R. sativus extract showed the highest inhibition (85%) of IL-8 secretion. DPPH analysis of the antioxidation properties of the ingredients showed that raw and seasoned A. ampeloprasum extracts exhibited the highest DPPH free radical scavenging activity (67.50 and 73.65%, respectively). These results suggest that seasoned ingredients used in Bibimbab have lower anti-inflammatory effects in relation to TNF-α and IL-6 secretion than raw ingredients in PMA-and A23187-treated HMC-1. In addition, the seasoned ingredients showed a tendency to increase antioxidative activity. Therefore, the ingredients used in Bibimbab have potential as anti-inflammatory and antioxidation agents.

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“…생리활성이 우수한 한약자원을 포함한 천연유래 생물자 원을 활용하기 위해서는 그 특징을 고려한 추출공정에 대한 검토가 필요하다 (12). 저렴한 비용과 짧은 시간에 동일한 양의 생물자원으로부터 다량의 기능성 성분을 생산할 수 있어야 한다 (13). 일반적으로 열수 추출은 식품의 추출방법 으로 많이 이용되고 있으나 가용성분 위주의 추출로 인해 낮은 추출수율, 고온으로 인한 에너지 소비 및 열에 의한 유용성분 파괴 등의 단점이 있다.…”

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Antioxidant and anti-aging activities of water extracts from Chionanthus retusus flesh according to different extraction temperatures

Choi¹,

Lee²,

Seo³

et al. 2017

Korean Journal of Food Preservation

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The fringe tree, Chionanthus retusus (Oleaceae) has been used as a natural remedy that helps a paralytic stroke, dementia, phlegm and malaria. This study was conducted to investigate the antioxidant and anti-aging activities of water extracts obtained from the flesh of C. retusus by reflux extraction at room temperature and 80℃, and pressure heating extraction at 110℃, for an increment of their practical use. The highest extraction yield from the flesh of C. retusus was 67.94% extract by pressure heating extraction at 110℃. The content of total polyphenol compounds was the highest by 72.71 mg/g in 80℃ extract by reflux extraction. The highest content of total flavonoid compounds was 7.60 mg/g at 110℃ extract. The results of soluble protein and reducing sugar contents showed the highest content of 4.93 mg/g and 46.77 mg/g in 110℃ extract, respectively. In the analysis of DPPH radical scavenging activity, all extracts showed an excellent effect of 92% in 1.0 mg/mL concentration. The highest effect of ABTS radical scavenging activity was 88.67% at 110℃ extraction in 1.0 mg/mL concentration. Nitrite scavenging ability was the highest as 66.16% at 110℃ extract at pH 1.2 and 1.0 mg/mL concentration. The results of SOD-like activity and xanthine oxidase inhibition activities showed the highest effect of 14.95% and 59.45% at 110℃ extract in 1.0 mg/mL, respectively. Tyrosinase inhibition activity for whitening effect was the highest of 35.31% at 85℃ in 1.0 mg/mL. In the analysis of anti-aging effect, collagenase and elastase inhibition activity showed the highest effects of 37.78% at 110℃ and 20.39% at 80℃, respectively. All results of antioxidant activities and anti-aging effects increased along with the concentration increases. These results indicated that the extracts extracted from the flesh of C. retusus at 80℃ and 110℃ have a large amount of useful ingredients, an excellent antioxidant activity, like as DPPH and ABTS radical scavenging activity, and anti-aging effects to develop functional products than those of 25℃.

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Antioxidant Activities of Ostrich Fern by Different Extraction Methods and Solvents

Cited by 31 publications

References 11 publications

Evaluation of the Wound-healing Activity of Rice Cell Extracts in Vitro

Evaluation of the Wound-healing Activity of Rice Cell Extracts in Vitro

Anti-inflammatory Effect and Antioxidative Activities of Ingredients used in Bibimbab

Antioxidant and anti-aging activities of water extracts from Chionanthus retusus flesh according to different extraction temperatures

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