Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis

Abstract

Dong Z., Tian G., Xu Z., Li M., Xu M., Zhou Y., Ren H. (2017): Antioxidant activities of peptide fractions derived from freshwater mussel protein using ultrasound-assisted enzymatic hydrolysis. Czech J. Food Sci., 35: 328-338.The freshwater mussel protein was hydrolysed using ultrasound-assisted enzymolysis. Ultrasound-assisted freshwater mussel protein hydrolysates (UPH) were divided into four fractions (> 10, 6-10, 3-6, and < 3 kDa) using ultrafiltration, and the fraction with the highest antioxidant activity was further subdivided into four fractions (F 1 -F 4 ) using gel chromatography. The amino acid compositions and antioxidant activities (DPPH, hydroxyl and superoxide radical scavenging activities, reducing power, ferrous ion chelating activity, and inhibition of linoleic acid oxidation) of peptide fractions were investigated. The results showed that the antioxidant activity of the < 3 kDa fraction was significantly higher than that of UPH, > 10, 6-10, and 3-6 kDa fractions. The antioxidant activity of F 2 was again higher compared with the < 3 kDa fraction and higher than that of F 1 , F 3 , and F 4 . Amino acid analysis showed that the antioxidant activities (except for chelating activity) of peptides increased with increasing hydrophobic amino acid content. The < 3 kDa and F 2 fractions exhibited strong inhibition of linoleic acid oxidation, their effects being even better than that of ascorbic acid (Vc) and l-glutathione (GSH). Therefore, these peptide fractions from freshwater mussel may be a potential natural antioxidant that could be added to various foods.

Inhibitory effect of low-molecular-weight peptides (0–3 kDa) from Spirulina platensis on H2O2-induced oxidative damage in L02 human liver cells

“…Several studies have reported that the bioactivity of peptide mixtures is largely affected by their molecular sizes and that the low-molecular-weight fractions exhibit more potent antioxidant activities, which may be due to the presence of hydrophobic amino acids in low-molecular-weight peptide. (Dong et al 2017 ). For example, low-molecular-weight whey hydrolysate peptides (0.1–2.8 kDa) have been found to be highly antioxidative and can protect lung fibroblast MRC-5 cells against H 2 O 2 -induced oxidative damage (Kong et al 2012 ).…”

Three Antimitotic Compounds Found in Banana Pseudo-Stem By Using Earthworms.

“…Biochemical tests to ascertain the phytochemicals of exudates revealed the presence of alkaloids, avonoids, anthraquinone, cardiac glycoside and phlorotannin's (Abdel Ghany et al 2019). Mass spectrometry analysis of banana stem extract with methanol (80%) showed the presence of many antidiabetic compounds (Dong et al 2017). Although different species of the banana stem was used for extraction with different solvents and examined for their cytotoxic activities; compounds responsible for cytotoxicity or antimitotic activity have not been identi ed or proposed yet.…”

Two Novel Multi-Functional Peptides from Meat and Visceral Mass of Marine Snail Neptunea arthritica cumingii and Their Activities In Vitro and In Vivo

“…However, the amino acid composition and peptide sequence are also important determinants of antioxidant activity. Peptides of freshwater mussels with a high molecular weight were reported to show relatively high antioxidant activity due to the abundant hydrophobic amino acids [26]. Indeed, hydrophobic and aromatic amino acids are known to play important roles in the antioxidant activity of peptides [27].…”

“…The hydroxyl radical scavenging activity of peptide fractions was determined according to the methods described by Dong et al [26]. Vitamin C was used as a positive control.…”