Antioxidant activity and anticancer effect of ethanolic and aqueous extracts of the roots of Ficus beecheyana and their phenolic components

Abstract

This study aimed to investigate the antioxidant and anticancer effects of ethanolic and aqueous extracts of the roots of Ficus beecheyana (EERFB and AERFB) and their phenolic components. In this study, total phenolic content and antioxidant activity of EERFB were higher than those of AERFB. Major phenolic compounds in the extracts were gallic acid, p-hydroxybenzoic acid, caffeic acid, chlorogenic acid, p-coumaric acid, and rutin; which were identified by high-performance liquid chromatography. Flow cytometric analysis of HL-60 cells exposed to EERFB showed that the percentage of apoptotic cells increased in a dose-dependent manner. EERFB treatment resulted in the loss of mitochondrial membrane potential and induced the apoptosis of HL-60 cells through a Fas- and mitochondrial-mediated pathway. Finally, pretreatment with general caspase-9/-3 inhibitors prevented EERFB from inhibiting cell viability in HL-60 cells. Our finding suggests that EERFB is an agent that may have antioxidant activity and inhibit the growth of cancer cells.

A Comprehensive Assessment of Bioactive Metabolites, Antioxidant and Antiproliferative Activities of Cyclocarya paliurus (Batal.) Iljinskaja Leaves

“…Our results corresponded with previous reports of Liu et al [20] and Zhou et al [17] who indicated that the antioxidant activity of C. paliurus varied with the geographical locations. The ethanol extracts of C. paliurus leaves showed stronger antioxidant capacity than the water extracts, which were in line with the extracts of F. beecheyana and S. chinensis [23,24]. Thus, the higher yields of bioactive compounds were likely to contribute to the stronger antioxidant property in the ethanol extracts.…”

“…Phytochemical contents, antioxidant and anticancer activities in plant extracts also vary with extraction solvents such as water, aqueous mixtures of ethanol, ethanol and methanol [21,22]. For example, antiproliferative activity on cancer cells was found in both ethanol and aqueous extracts of Ficus beecheyana and Saururus chinensis root, however the ethanol extracts showed better antioxidant activity [23,24]. The phenolic compounds, anticancer and antioxidant activities of indigo plants also varied with the extraction solvents [25].…”

Evaluation of Phytochemical Compositions and Biological Properties of Achillea gypsicola at Different Phenological Stages

“…The antioxidant activity of the extracts can be caused by the combined effect of these phenolic compounds and flavonoids; thus, it can be prevented free radical mediated diseases. It is known that rutin and quercetin, p ‐coumaric, caffeic acid and chlorogenic acid have strong antioxidant activities. Differences of polyphenol amounts among samples can be caused by harvested plants at different phenological stages.…”

Anti-Tumor Activity of Dry Extract Padus Grayanae Maxim in Vitro

“…Так, в работе Dong Y et al [15] показана высокая активность флавоноидов против клеточной линии опухолевых клеток аденокарциномы желудка человека (AGS). В литературе также имеются данные, что хлорогеновая и кофейная кислоты, входящие в состав сухого экстракта Padus Grayanae Maxim, проявляют антираковый эффект в отношении клеток лейкемии HL-60, незначительно ингибируют рост клеток AGS и практически не действуют на HepG2 [16].…”