Antioxidant Activity and Inhibitory Effect of Aster scaber Thunb. Extract on Adipocyte Differentiation in 3T3-L1 Cells

Abstract

Clinical and preclinical trials of involving drugs with anti-obesity effects have focused on screening for herbal medicines suspected to have anti-obesity activities. In this study, an extract of Aster scaver Thunb., which was prepared in 80% methanol (ASE), was assessed for its total phenol content, total flavonoid content, antioxidant activity ability to scavenge the α-α-diphenyl-β-picrylhydrazyl, 2,2'-azino-bis-[3-ethylbenzthiazoline]-6-sulfonic acid radical, and anti-adipogenic effects. The anti-adipogenic effect of ASE on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by assaying the suppression of adipocyte differentiation and lipid accumulation by using western blot analysis and the Oil RedO assay, respectively. The staining results showed that ASE significantly inhibited 3T3-L1. Western blot analysis results showed that ASE decreased the levels of peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein 1c. These results demonstrate that ASE directly inhibits the differentiation of preadipocytes, and might be an important adjunct in the therapeutic efforts to reduce adipogenesis.

Antioxidant and anti-adipogenic activities of chestnut (Castanea crenata) byproducts

“…Previous studies have reported that the anti-adipogenic activities of Rumex crispus L. and Aster scaber Thunb. were associated with downregulated mRNA expression levels of transcription factors such as C/ EBP and PPAR families (31,32). Thus, based on the results, it was possible to conclude that 3T3-L1 adipogenesis was inhibited by WE and ME treatments through suppression of transcriptional factors including C/EBPβ, PPARγ, and C/EBPα levels.…”

Beneficial role and function of fisetin in skin health via regulation of the CCN2/TGF-β signaling pathway

“…Furthermore, fisetin treatments led to inhibition of adipocytes-specific gene expression, including FAS and LPL. Anti-adipogenic activities of extracts from the natural materials Rumex crispus L. (a perennial flowering plant in the family Polygonaceae) and Aster scaber Thunb (a perennial herb of the Compositae family) were reportedly associated with inhibited mRNA expression levels of the transcription factors C/EBPs and PPARγ (27,28). Regulation of adipogenic transcriptional factors and adipocyte-specific genes reportedly plays an important role for inhibition of lipid accumulation (29,30).…”

Anti-inflammatory and Anti-oxidant Activities of Aster Scaber Ethanol Extract

“…A study has reported varying levels of anti-oxidant profiles in blanched, pan-fired and microwaved AS extract (Kim et al, 2014). AS ethanol extract has been reported to possess the anti-oxidant activity by reducing DPPH and antiadipogenic activity by suppressing the preadipocyte conversion in 3T3L1 cells (Choi et al, 2013). Hydrogen peroxide induced cell death in brain neuroblastoma SK-N-SH cells was also reported to be reversed by AS extract (Chung et al, 2016).…”