Antioxidant Activity and Oxidative Stress-Oriented Apoptosis Pathway in Saccharides Supplemented Cryopreserved Sperm of Pacific Abalone, Haliotis discus hannai

Hossen, Shaharior; Sukhan, Zahid Parvez; Cho, Yusin; Lee, Won Kyo; Kho, Kang Hee

doi:10.3390/antiox11071303

Cited by 13 publications

(7 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may have been influenced by an increase in LPO, thereby decreasing the sperm quality [ 33 ]. Moreover, decreased mRNA expression of antioxidant genes such as GPx and SOD might result in apoptosis [ 34 ], as shown by the higher number of sperm with membrane damage, acrosome loss, and broken DNA ( Table-2 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Widyastuti,

Prastowo,

Jaswandi

et al. 2024

Vet World

View full text Add to dashboard Cite

Background and Aim: Semen storage is an important reproductive method used in artificial livestock breeding. However, oxidative stress during storage reduces the quality of sperm. Melatonin supplementation in semen storage medium has not been well studied, but it has been shown to protect cells from oxidative stress. Therefore, this study aimed to determine the effect of melatonin supplementation on sperm quality parameters and antioxidant gene expression levels in semen extenders during cold storage. Materials and Methods: Semen extenders with melatonin concentrations of 0 (control), 0.1, 0.2, and 0.3 mM were added as treatment. The treated semen was then stored at 5°C for 72 h using a cold storage method, and quality parameters, including percentage of progressive motility, membrane integrity, intact acrosome, and DNA integrity, were measured every 24 h. In addition, messenger ribonucleic acid abundance levels of glutathione peroxidase (GPx) and superoxide dismutase (SOD) genes were sampled after 0 and 72 h of cold storage. Results: All observed sperm quality parameters decreased with increasing cold storage time; however, 0.2 mM melatonin demonstrated superior protection of sperm quality during cold storage. Gene expression analysis showed that GPx levels decreased significantly (p < 0.05) after 72 h in semen without melatonin but not in the melatonin-treated groups. A similar trend was also observed in SOD, indicating that exogenous antioxidants effectively protected the sperms. Conclusion: Melatonin supplementation at 0.2 mM in semen extenders during cold storage maintains sperm quality parameters for up to 72 h because melatonin protects sperm from oxidative stress. These findings can be used to improve the semen storage protocol by combining semen extender and antioxidant. Keywords: antioxidant gene expression, melatonin, semen cold storage, sperm quality.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Widyastuti,

Prastowo,

Jaswandi

et al. 2024

Vet World

View full text Add to dashboard Cite

show abstract

“…Then, the suspension was mixed with 2.5 mL thiobarbituric acid and heated at 100 °C for 15 min, followed by quickly cooling in an ice bath. After a 10 min centrifugation (5000 r/min), the absorbance of the supernatant was measured at 450, 532, and 600 nm, respectively [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Concurrent Production of α- and β-Carotenes with Different Stoichiometries Displaying Diverse Antioxidative Activities via Lycopene Cyclases-Based Rational System

Luo

Dai

et al. 2022

Antioxidants

View full text Add to dashboard Cite

α- and β-carotenes belong to the most essential carotenoids in the human body and display remarkable pharmacological value for health due to their beneficial antioxidant activities. Distinct high α-/β-carotene stoichiometries have gained increasing attention for their effective preventions of Alzheimer’s disease, cardiovascular disease, and cancer. However, it is extremely difficult to obtain α-carotene in nature, impeding the accumulations of high α-/β-carotene stoichiometries and excavation of their antioxidant activities. Herein, we developed a dynamically operable strategy based on lycopene cyclases (LCYB and LCYE) for concurrently enriching α- and β-carotenes along with high stoichiometries in E. coli. Membrane-targeted and promoter-centered approaches were firstly implemented to spatially enhance catalytic efficiency and temporally boost expression of TeLCYE to address its low competitivity at the starting stage. Dynamically temperature-dependent regulation of TeLCYE and TeLCYB was then performed to finally achieve α-/β-carotene stoichiometries of 4.71 at 37 °C, 1.65 at 30 °C, and 1.06 at 25 °C, respectively. In the meantime, these α-/β-carotene ratios were confirmed to result in diverse antioxidative activities. According to our knowledge, this is the first time that both the widest range and antioxidant activities of high α/β-carotene stoichiometries were reported in any organism. Our work provides attractive potentials for obtaining natural products with competitivity and a new insight on the protective potentials of α-/β-carotenes with high ratios for health supply.

show abstract

“…Representative antioxidant enzymes operating in this context include superoxide dismutase (SOD) and catalase (CAT). SOD converts O 2 − to H 2 O 2 , which is further converted into nontoxic H 2 O and O 2 molecules by CAT (Hossen et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Melatonin injection and red light irradiation affect the antioxidant response and cell damage in disk abalone (Haliotis discus hannai) exposed to high water temperatures

Kim,

Park

et al. 2024

J Exp Zool Pt A

View full text Add to dashboard Cite

The effects of red light‐emitting diode (LED) light irradiation (630 nm, 0.5 W/m2) and melatonin (10−8 and 10−7 M) on oxidative stress and physiological responses in abalones exposed to high temperatures (28°C) were investigated. Changes in messenger RNA (mRNA) expressions of melatonin receptor (MT‐R), heat shock protein 70 (HSP70), and antioxidant enzymes, as well as alterations in H2O2 levels in the hemolymph, were examined. The results revealed that high‐temperature‐stressed abalones treated with melatonin injections or exposed to red LED light showed a significant increase in MT‐R mRNA expression, while HSP70 mRNA expression decreased. Notably, HSP70 mRNA expression levels in the red LED light‐irradiated group were similar to those in the group injected with 10−8 M melatonin after 24 h exposure. Abalones treated with melatonin at 20°C or irradiated with red LED light exhibited decreased H2O2 levels and reduced antioxidant enzyme mRNA expression compared with those of the control group. However, the high‐temperature environment induced oxidative stress in abalones, leading to increased antioxidant enzyme mRNA expression compared with that under 20°C conditions. Moreover, abalones exposed to high‐temperature stress exhibited hepatopancreatic DNA damage, which was attenuated by melatonin treatment or red LED light irradiation. Hence, red LED light reduces oxidative stress, boosts antioxidant enzymes, and alleviates DNA damage in high‐temperature‐stressed abalones, akin to 10−8 M melatonin treatment. Therefore, considering the practical challenges of continuous melatonin administration to abalones, utilizing red LED light emerges as a practical, effective alternative to protect abalones from oxidative stress compared to 10−8 M melatonin treatment.

show abstract

Antioxidant Activity and Oxidative Stress-Oriented Apoptosis Pathway in Saccharides Supplemented Cryopreserved Sperm of Pacific Abalone, Haliotis discus hannai

Cited by 13 publications

References 69 publications

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Effect of melatonin supplementation on sperm quality parameters and expression of antioxidant genes during cold storage of buck semen extenders

Concurrent Production of α- and β-Carotenes with Different Stoichiometries Displaying Diverse Antioxidative Activities via Lycopene Cyclases-Based Rational System

Melatonin injection and red light irradiation affect the antioxidant response and cell damage in disk abalone (Haliotis discus hannai) exposed to high water temperatures

Contact Info

Product

Resources

About