Antioxidant activity and phenolic content of the Indian wheat varieties

Narwal, Sneh; Thakur, Vidisha; Sheoran, Sonia; Dahiya, Shashi; Jaswal, S. V.; Gupta, Raj Kumar

doi:10.1007/s13562-012-0179-1

Cited by 22 publications

(17 citation statements)

References 23 publications

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“…Much research on the antioxidant effect of different microalgae has been carried out (Safafar et al, 2015;Singh et al, 2016). Polyphenol and flavonoid contents are commonly measured components referring to antioxidant activity (Pan et al, 2007;Saeed et al, 2012;Li et al, 2014;Narwal et al, 2014;Ying et al, 2017). Previous studies also verified the relationships between algal phenols and antioxidant capacity (Hajimahmoodi et al, 2010).…”

Section: Discussionmentioning

confidence: 96%

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Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.

Wang

Sun

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Crypthecodinium cohnii (dinoflagellate) and Schizochytrium sp. (thraustochytrid) are the main sources for docosahexaenoic acid (DHA). The present study aimed to evaluate the antioxidant activity of petroleum ether, ethyl acetate, n-butanol, and water fractions of alcohol aqueous extracts of these two microalgae and to provide a theoretical basis for comprehensive utilization. The antioxidant activity was determined by total antioxidant capacity (TAC) determination, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferrous ion-chelating ability (FICA) assay, and reducing power (RP) assay. The total phenolic content (TPC) and total flavonoid content (TFC) were also measured by the Folin-Ciocalteu and spectrophotometry methods, respectively. The results indicated that the extracts from these two microalgae possessed good antioxidant capacity. Analysis showed that most antioxidant performance indicators (TAC, DPPH, and RP) were positively correlated with the TPC of the extracts, suggesting that the phenolics might be the major components in C. cohnii and Schizochytrium sp., contributing to their antioxidative function. Therefore, the polar fractions of C. cohnii and Schizochytrium sp. could be further examined and considered for application in health products or cosmetics.

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Section: Discussionmentioning

confidence: 96%

“…A DPPH radical scavenging activity assay was performed as described by Narwal et al (2014). DPPH (3 ml, 0.08 mmol/L) was added to the sample solutions and then mixed.…”

Section: Antioxidant Capacity Assaymentioning

confidence: 99%

Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.

Wang

Sun

et al. 2017

J. Zhejiang Univ. Sci. B

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“…Body of humans encloses various endogenous substances which scavenge free radicals; however, a significant portion is contributed through intake also. Whole grains, fruits, and vegetables possess enormous kinds of phytochemicals and most of them are liable for the antioxidative action of these foodstuffs (Narwal et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Seed Biochemical Analysis Based Profiling of Diverse Wheat Genetic Resource from Pakistan

Khalid

Hameed

2017

Front. Plant Sci.

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Wheat is the major nutrient source worldwide. In Pakistan, it has a crucial place in agriculture as well as in national economy. For seed biochemical compositional analysis, wheat germplasm (77 genotypes) was collected from different agro-climatic zones of Pakistan. Significant variation (p < 0.05) was observed for tested parameters among tested genotypes. Highest activity of ascorbate peroxidase (APX) was detected in Pavon (1,426.67 Units/g s. wt.), catalase (CAT) in Pasban-90 (633.33 Units/g s. wt.), peroxidase (POD) in IQBAL-2000 (42,579.6 Units/g s. wt.), and superoxide dismutase (SOD) in Manthar-2003 (278.93 Units/g s. wt.). Whereas, maximum activity of alpha amylase was found in SH-2002 (292.70 mg/g s. wt.), esterase in Dharabi 2011 (987.80 μM/min/g s. wt.), and protease in NR-234 (11,183.33 Units/g s. wt.) and highest total oxidant status (TOS) was detected in Faisalabad-2008 (390.0 μM/g s. wt.), malondialdehyde (MDA) content in Margalla-99 (679.23 μM/g s. wt.), total phenolic content (TPC) in Bhakkar-2000 (25,383.33 μM/g s. wt.), and ascorbic acid (AsA) content in SH-2002 (713.0 μg/g s. wt.). Maximum total soluble sugar was found in Saleem-2000 (29.86 mg/g s. wt.), reducing sugars in Punjab-96 (12.68 mg/g s. wt.), non-reducing sugars in Saleem-2000 (27.33 mg/g s. wt.). However, highest albumins was identified in TC-4928 (352.89 mg/g s. wt.) and globulins in MEXI PAK (252.67 mg/g s. wt.), salt soluble proteins in Faisalabad-2008 (162.44 mg/g s. wt.), and total soluble proteins in Punjab-96 (487.33 mg/g s. wt.) indicating good quality of wheat genotypes as well as good nutritional status. Genotypes which have been ranked high in respective parameter can be employed in breeding to enhance the nutritional quality of wheat.

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“…Besides this, it is also considered as natural source of both enzymatic and non-enzymatic antioxidants. The enzymatic antioxidants include superoxide dismutase (SOD), glutathione reductase (GR), and ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) while non-enzymatic antioxidants include vitamin C (tocopherols and tocotrienols), vitamin E, and carotenoids [6].…”

Section: Introductionmentioning

confidence: 99%

Protein quantification and enzyme activity estimation of Pakistani wheat landraces

et al. 2020

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Wheat is a major food grain in Pakistan having a prominent role in agriculture as well as the economic status of the country. In the current study, seeds of 99 wheat landraces were characterized for the quantification of seed storage proteins (Albumins, Globulin, Gliadins, and Glutenin), enzyme activities of antioxidant enzymes i.e. Ascorbate peroxidase (APX), Catalase (CAT), Superoxide dismutase (SOD), Peroxidase (POD), one hydrolytic enzyme Protease (PROT) and non-enzymatic antioxidant enzyme Ascorbic acid (AsA). The landraces were categorized into low, medium, and high based on protein concentration and enzymes activities/content. The majority of the landraces were placed in the medium category. However, for the AsA parameter majority of the landraces were placed in the low category. The highest concentration of total extracted protein (184.88±0.7 mg/g. wt.), globulins (21.35 ±0.43 mg/g. wt.) and glutenin (20±0.04 mg/g. wt.) as well as the high activity of SOD (303 ±16.80 Units/g. wt.), and Ascorbic acid (533±36.1 Units/g. wt.) was identified in the wheat landrace "11757" collected from district Panjgur (Balochistan). The wheat landrace "11760", collected from district Kech (Balochistan), contained the highest albumins concentration (65.42±0.02 mg/g. wt.) and highest activity for CAT (589.5±61.20 Units/g. wt.). The highest activity of POD (32341± 91.3) and PROT was observed in seeds of the wheat landrace "11618" collected from the Gilgit Baltistan region of Pakistan. The principal component analysis showed that the great variations existed for the tested parameters among the wheat landraces. The landraces with a high concentration of seed storage proteins and antioxidant enzyme activities can be used for breeding purposes to improve the nutrimental quality of wheat cultivars.

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Antioxidant activity and phenolic content of the Indian wheat varieties

Cited by 22 publications

References 23 publications

Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.

Antioxidant activity of alcohol aqueous extracts of Crypthecodinium cohnii and Schizochytrium sp.

Seed Biochemical Analysis Based Profiling of Diverse Wheat Genetic Resource from Pakistan

Protein quantification and enzyme activity estimation of Pakistani wheat landraces

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