Antioxidant activity and total phenolic content of an isolated Morinda citrifolia L. methanolic extract from Poly-ethersulphone (PES) membrane separator

Objective. Artichoke (Cynara scolymus L.) was one of the plant remedies for primary health care. The present study was focused on the determination of chemical composition, antioxidant activities, and anti-inflammatory activity and on analyzing its major bioactive polyphenols by HPLC. Methods. Artichoke Leaves Extracts (ALE) were analyzed for proximate analysis and phytochemical and antioxidant activity by several methods such as DDPH, ABTS, FRAP, and beta-carotene bleaching test. The carrageenan (Carr) model induced paw oedema in order to investigate the anti-inflammatory activity. Identification and quantification of bioactive polyphenols compounds were done by HPLC method. The oxidative stress parameters were determined; CAT, SOD, GSH, MDA, and AOPP activities and the histopathological examination were also performed. Results. It was noted that EtOH extract of ALE contained the highest phenolic, flavonoid, and tannin contents and the strongest antioxidants activities including DDPH (94.23%), ABTS (538.75 mmol), FRAP assay (542.62 umol), and β-carotene bleaching (70.74%) compared to the other extracts of ALE. Administration of EtOH extract at dose 400 mg/kg/bw exhibited a maximum inhibition of inflammation induced by Carr for 3 and 5 hours compared to reference group Indomethacin (Indo). Conclusion. ALE displayed high potential as natural source of minerals and phytochemicals compounds with antioxidant and anti-inflammatory properties.

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“…Many researches revealed that flavonoids and polyphenols displayed the highest ability of scavenging activity in medicinal plants [37, 38]. …”

Section: Discussionmentioning

confidence: 99%

Chemicals Compositions, Antioxidant and Anti‐Inflammatory Activity of Cynara scolymus Leaves Extracts, and Analysis of Major Bioactive Polyphenols by HPLC

Salem

Affes

Athmouni

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…These structural features determine the complexing, antioxidant, and biological properties of tannins [50][51][52] . The specific phenolic substitution patterns result in different responses to the commonly used analytical protocols based on the determination of the phenolic groups' content or protein precipitation [53][54][55] . This implies that the overall amount of proanthocyanidin or phenolic groups in a sample cannot be reported as a single value and does not allow the use of specific standards.…”

Section: Nature Protocolsmentioning

confidence: 99%

Determination of hydroxyl groups in biorefinery resources via quantitative 31P NMR spectroscopy

et al. 2019

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The analysis of chemical structural characteristics of biorefinery product streams (such as lignin and tannin) has advanced substantially over the past decade, with traditional wet-chemical techniques being replaced or supplemented by NMR methodologies. Quantitative 31 P NMR spectroscopy is a promising technique for the analysis of hydroxyl groups because of its unique characterization capability and broad potential applicability across the biorefinery research community. This protocol describes procedures for (i) the preparation/solubilization of lignin and tannin, (ii) the phosphitylation of their hydroxyl groups, (iii) NMR acquisition details, and (iv) the ensuing data analyses and means to precisely calculate the content of the different types of hydroxyl groups. Compared with traditional wet-chemical techniques, the technique of quantitative 31 P NMR spectroscopy offers unique advantages in measuring hydroxyl groups in a single spectrum with high signal resolution. The method provides complete quantitative information about the hydroxyl groups with small amounts of sample (~30 mg) within a relatively short experimental time (~30-120 min).

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“…Krishnaiah et al (2013) Rufino et al (2010), que estudaram o conteúdo de fenólicos totais em diversos frutos, classificaram os frutos em três categorias: baixo (<100 mg EAG/g), médio (100-500 mg EAG/g) e alto mg EAG/g (>500 mg EAG/g). De acordo com essa classificação, os extratos aquoso, EtOH e MeOH/Ace são considerados com alto teor de compostos fenólicos.…”

Section: Análise (%)unclassified

Composição centesimal, compostos bioativos e atividade antioxidante de frutos de Morinda citrifolia Linn (noni) cultivados no Paraná

Palioto

Silva

Mendes

et al. 2015

Rev. bras. plantas med.

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Recebido para publicação em 03/09/2013 Aceito para publicação em 28/05/2014 RESUMO. O fruto de noni (Morinda citrifolia L.) é consumido há milênios na medicina popular polinésia devido aos benefícios nutricionais e terapêuticos. O consumo de noni em outros países, incluindo o Brasil, cresceu vertiginosamente nos últimos anos em decorrência das atividades biológicas atribuídas a ingestão do suco da fruta, principalmente pela propriedade anticâncer. Contudo, a composição química da planta, que está relacionada com suas propriedades biológicas, é determinada pelo seu local de origem, e por influência do clima e do solo onde é cultivada. Neste sentido, este trabalho teve como objetivo analisar a polpa extraída de frutos maduros de noni cultivados em Maringá-PR. A análise da polpa in natura apresentou 89,16% ABSTRACT: Proximate composition, bioactive compounds and antioxidant activity of fruits of Morinda citrifolia L. (noni) cultivated in Paraná, Brazil. The noni fruit (Morinda citrifolia L.) has been consumed for millennia in the Polynesian folk medicine because of its nutritional and therapeutic benefits. The consumption of the noni fruit in other countries, including Brazil, has increased in recent years because of the biological activities attributed to the ingestion of noni juice, especially the anticancer effect. However, the chemical composition of the plant, which is related to its biological properties, is determined by its geographical origin and is influenced by climate and soil. Therefore, this study aimed to analyze the pulp extracted from noni fruit grown in Maringá, state of Paraná, Brazil. The fresh pulp analysis showed 89.16% of moisture, 0.75% of ash, 2.10% of protein, 2.19% of fat and 5.81% of carbohydrates. The bioactive compounds analyzed were anthocyanins (1.39 mg.100 g -1 pulp), yellow flavonoids (13.01 mg.100 g -1 pulp), carotenoids (0.45 mg.100 g -1 pulp) and vitamin C (12.16 mg.100 g -1 pulp). For the analysis of total phenols and antioxidant activity, different extracts were prepared. The highest total phenolic contents were found in the aqueous extract (1143.56 mg EAG.100 g -1 ), followed by the ethanol extract (966.96 mg EAG.100 g -1 ), methanol/acetone extract (820.88 mg EAG.100 g -1 ) and methanol extract (306.33 mg EAG.100 g -1 ). The results for the antioxidant capacity were determined by the EC 50 , which is concentration of extract required to reduce 50% of the DPPH radical. The best results for the antioxidant capacity were found in the methanol/ acetone extract (EC 50 of 25.18 mg.mL -1 ) and in the methanol extract (EC 50 of 25.96 mg.mL -1 ). The antioxidant activity of the fruit may be related to its vitamin C content, since the extracts with lower total phenolic content were those that had lower EC 50 values.

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Antioxidant activity and total phenolic content of an isolated Morinda citrifolia L. methanolic extract from Poly-ethersulphone (PES) membrane separator

Cited by 43 publications

References 17 publications

Chemicals Compositions, Antioxidant and Anti‐Inflammatory Activity of Cynara scolymus Leaves Extracts, and Analysis of Major Bioactive Polyphenols by HPLC

Chemicals Compositions, Antioxidant and Anti‐Inflammatory Activity of Cynara scolymus Leaves Extracts, and Analysis of Major Bioactive Polyphenols by HPLC

Determination of hydroxyl groups in biorefinery resources via quantitative 31P NMR spectroscopy

Composição centesimal, compostos bioativos e atividade antioxidante de frutos de Morinda citrifolia Linn (noni) cultivados no Paraná

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