Antioxidant Activity Derived from Marine Green‐Lipped Mussel Perna canaliculus Extracts in Mice

Abstract

This study investigates the antioxidant activities of lipid, protein, and carbohydrate extracts from the marine mollusk Perna canaliculus. Lipids were extracted using acetone, which was followed by protein extraction using the broad-spectrum enzyme Alcalase and then carbohydrate extraction using cetylpyridinium chloride. Eighty white BALB/c mice were divided into eight groups according to the administered extracts. Groups 1 and 5 were the control and toxin control groups, respectively. Groups 2, 3, and 4 were administered lipid, protein, and carbohydrate extracts, respectively. The other groups were administered P. canaliculus extracts as well as gentamicin and acetaminophen, known as ethanolic extracts, derived from Nerium oleander to induce oxidation stress. All groups showed significant improvements in body weight ( p < 0.05 ). The lipid extract group showed a significant decrease in low-density lipoprotein cholesterol ( p < 0.05 ) and a significant increase in high-density lipoprotein cholesterol ( p < 0.05 ). After the toxin injection, all groups treated with P. canaliculus extracts showed increased antioxidant effects on hepatocytes ( p < 0.05 ). The lipid extracts induced antioxidant effects to protect the kidney by increasing lipid peroxidation ( p < 0.05 ) and catalase activities ( p < 0.05 ). Also, protein extracts showed antioxidant effects by increasing glutathione and catalase levels significantly ( p < 0.005 ). In conclusion, P. canaliculus extracts, especially lipids and proteins, have potent antioxidant activities that protect vital organs from oxidation stress.

Mussel-Based Biomimetic Strategies in Musculoskeletal Disorder Treatment: From Synthesis Principles to Diverse Applications

“… 100 Interestingly, antioxidants in green-lipped mussels were proven to mediate the free radicals, which were the typical indicators in inflamed joints. 101 , 102 Therefore, a variety of injectable materials have been developed to fill the joint cavity, capable of inflammatory factor elimination especially in the liquid form. For instance, Chen’s 103 study demonstrated that GelMA hydrogel implantation combined with CM-SIN injection was a promising strategy for inducing autophagy and ameliorating osteoarthritis cartilage degradation.…”

Pro-Apoptotic Activity of Epi-Obtusane against Cervical Cancer: Nano Formulation, In Silico Molecular Docking, and Pharmacological Network Analysis

“…Additionally, they have numerous unique scaffolds, which afford more and more inspiration for drug discovery [16][17][18][19][20][21][22][23][24][25]. Though terrestrial plants as well as bacteria have usually been the main resources of natural metabolites, in the last 30 years, marine ecosystems, including their invertebrates, plants, as well as bacteria, have also been the main sources of numerous novel structural metabolites, which are identified as natural marine products [26][27][28][29][30][31][32].…”