Antioxidant Activity Enhancement Effect of Silver-Ionized Water: Silver Cation Prepared by Electrolysis

Abstract

In the present study, tap water, alkaline electrolyzed water (AlEW) and tourmaline water (TMW) were used as the electrolytes to generated the silver-ionized water (SIW), AlEW-SIW and TMW-SIW, respectively. The antioxidant properties of the samples containing ascorbic acid (AsA) were investigated by WST-kit method. The results showed that the SOD activity of AsA (2 mmol/L) dissolved in SIW (66.0%) was enhanced by about 8% compared to that of the tap water (57.9%). The SOD activity of the AlEW-SIW solution (77.3%), which was higher than that of the SIW solution, and lower than that of the AlEW solution (83.6%). The SOD activity of the TMW-SIW solution (83.0%) was similar to that of the TMW solution (82.5%). Furthermore, to classify the sample solutions, discriminant analyses were performed based on near infrared (NIR) spectral data, which was consistent with the results of the WST-kit method. The SOD activity of the AlEW-SIW and TMW-SIW solutions decreased slowly with storage time, and their SOD activities were higher than that of AlEW, TMW and the tap water solutions at storage time of 14 days. In summary, AlEW-SIW and TMW-SIW showed similar antioxidant activity enhancement as AlEW and TMW, and they also maintained the stability of the antioxidant activity of AsA during storage.

Aquaphotomics Investigation of Electrolyzed Hydrogen Water: Understanding the Water Molecular Structure and Its Role in Scavenging Reactive Oxygen Species

“…However, the spotlight is increasingly turning towards emerging functional waters such as alkaline electrolyzed water, acidic electrolyzed water, electrolyzed hydrogen, hydrogen-enriched water, and tourmaline water. These waters are gaining attention not only for their potential health benefits but also because their distinctive properties arise from artificial treatments, not from the addition of substances with antioxidative properties [9,[11][12][13][14][15][16] Despite the increasing production and effectiveness of these newer options, the intricate mechanisms underlying their antioxidant and other health effects continue to be the subject of ongoing and intensive scientific exploration [9,[11][12][13]17]. Japan has emerged as a frontrunner in these initiatives; in 1993, the Japanese Society for Functional Water (JSFW) was established and serves as a national academic center dedicated to acquiring, accumulating, and disseminating accurate knowledge regarding functional water [18].…”

“…Tourmaline water or also called tourmaline-modified water (TMW) is a functional water with electrochemical properties produced by water passing through stones made of tourmaline, a natural borosilicate mineral that releases negative ions and generates an electric field on its surfaces [9]. TMW was also shown to enhance antioxidant activity of well-known antioxidant, ascorbic acid [11,29].…”

“…With high values of DH, along with high pH, low DO, and an extremely negative ORP, it is believed that these characteristics contribute to the stability and antioxidative properties of ERW functional water, protecting biological macromolecules from oxidative damage [3]. Other proposed agents in this mechanism behind the antioxidant and SOD activity are silver ions [11] or platinum particles [19] , and generally greater difference in ionic mobility between cations and anions [30].…”

“…Near-infrared spectroscopy (NIRS) was employed in prior research to classify various waters in the context of functional water exploration [11]. The research study used tap water, AlEW, and TMW as electrolytes to generate silver-ionized water (SIW) and mixtures AlEW-SIW, and TMW-SIW, respectively.…”

“…The research study used tap water, AlEW, and TMW as electrolytes to generate silver-ionized water (SIW) and mixtures AlEW-SIW, and TMW-SIW, respectively. The spectra of these aqueous solutions were acquired in the visible and near infrared (NIR) region spanning 400 -1100 nm [11]. Within this range, the NIR segment encompasses the 3rd and 2nd overtones of water stretching vibrations [38], providing a valuable opportunity to observe the molecular structure of water.…”