Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases

Wang, Shihan; Gan, Yuanshuai; Mao, Xinxin; Hong, Kan; Li, Nan; Zhang, Changli; Wang, Zhihan; Wang, Yongsheng

doi:10.3390/molecules26061625

Cited by 10 publications

(6 citation statements)

References 52 publications

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“…An 1% (w/v) protein solution was incubated at a room temperature for 30 min at different pH from 2 to 12, which were adjusted using a 1 M HCl and 1 M NaOH solution, and centrifuged at 13,000 rpm for 25 min (Chen et al, 2018;Wang et al, 2021). The protein content in the supernatant was measured using the BCA protein assay kit (Pierce BCA protein Assay Kit, Thermo Scientific, Rockford, IL, USA).…”

Section: Determination Of Solubilitymentioning

confidence: 99%

Characterization of Yeast Protein Hydrolysate for Potential Application as a Feed Additive

Min,

Lee,

Kang

et al. 2024

Food Sci Anim Resour

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Yeast protein can be a nutritionally suitable auxiliary protein source in livestock food. The breakdown of proteins and thereby generating high-quality peptide, typically provides nutritional benefits. Enzyme hydrolysis has been effectively uesed to generate peptides; however, studies on the potential applications of different types of enzymes to produce yeast protein hydrolysates remain limited. This study investigated the effects of endo- (alcalase and neutrase) and exotype (flavourzyme and prozyme 2000P) enzyme treatments on yeast protein. Endotype enzymes facilitate a higher hydrolysis efficiency in yeast proteins than exotype enzymes. The highest degree of hydrolysis was observed for the protein treated with neutrase, which was followed by alcalase, prozyme 2000P, and flavourzyme. Furthermore, endotype enzyme treated proteins exhibited higher solubility than their exotype counterparts. Notably, the more uniform particle size distribution was observed in endotype treated yeast protein. Moreover, compared with the original yeast protein, the enzymatic protein hydrolysates possessed a higher content of β-sheets structures, indicating their higher structural stability. Regardless of enzyme type, enzyme treated protein possessed a higher total free amino acid content including essential amino acids. Therefore, this study provides significant insights into the production of protein hydrolysates as an alternative protein material.

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Section: Determination Of Solubilitymentioning

confidence: 99%

Characterization of Yeast Protein Hydrolysate for Potential Application as a Feed Additive

Min,

Lee,

Kang

et al. 2024

Food Sci Anim Resour

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show abstract

“…This was the case with apricot kernel ( Semen Armeniacae Amarum ) hydrolysate that was obtained by hydrolysis and degreasing with the compound protease of alkaline protease and Flavourzyme [ 17 ]. Some studies have used trypsin, Flavourzyme, and neutral and alkaline protease to extract antioxidant proteins from frog breast oil ( Ranae Oviductus ) [ 18 ].…”

Section: Extraction Of Peptidesmentioning

confidence: 99%

Review on Extraction, Modification, and Synthesis of Natural Peptides and Their Beneficial Effects on Skin

Tsopmejio

Wang

et al. 2023

Molecules

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Peptides, functional nutrients with a size between those of large proteins and small amino acids, are easily absorbed by the human body. Therefore, they are seeing increasing use in clinical medicine and have revealed immunomodulatory and anti-inflammatory properties which could make them effective in healing skin wounds. This review sorted and summarized the relevant literature about peptides during the past decade. Recent works on the extraction, modification and synthesis of peptides were reviewed. Importantly, the unique beneficial effects of peptides on the skin were extensively explored, providing ideas for the development and innovation of peptides and laying a knowledge foundation for the clinical application of peptides.

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“…The differences of amino acid composition as well as variances in amino acids with donating electrons are important factor that provoked antioxidant alterations of samples. Wang et al (2021) carried out an investigation on the antioxidant activity of Oviductus Ranae protein and according to their report, the enzymatic hydrolysates of Oviductus Ranae protein revealed high antioxidant activity than the protein without enzymolysis, and the optimum hydrolysis time of different proteases was attained between 180-240 min.…”

Section: Amino Acid Constitutionsmentioning

confidence: 99%

Stabilization of Chlorella Bioactive Hydrolysates Within Biopolymeric Carriers: Techno-functional, Structural, and Biological Properties

et al. 2023

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Chlorella vulgaris (CV) is a green unicellular microalga with various bioactive compounds (BACs) and is highly appreciated due to its variety of biological activities. Among different sources, bioactive peptides have positive biological effects and numerous health-promoting properties. However, instability, reactivity and high bitterness are the main challenges which may restrict the application of these components in preparation of diverse food compounds. Therefore, the present investigation stresses on the capability of the gum arabic (GA), maltodextrin (MD), pectin (PE) and alginate (AL), and combinations of them as carrier agents on physicochemical, techno-functional, structural and activity of spray-dried CV peptides. Biological indices were affected by the degree of hydrolysis and free amino acid values. The alteration in physico-chemical, functional, owability along with color characteristics may be associated with composition of the carriers. MD-AL combination showed the best performance on the mentioned indicators, physical stability and scavenging the ABTS (84%) and DPPH (77%) radicals of spray-dried (SD) peptides. The chemical structures (FTIR analysis) indicated the placement of peptides in the carrier matrix. SEM images of SD particles revealed wrinkled, irregular and hollow structures (matrix-type microsphere) in different sizes. Referring to the ndings, microencapsulated CV peptides is a valuable source of BACs in functional formulations. MaterialSample (Chlorella powder) was purchased from Iranian National Algae Culture Collection (INACC). The chemicals including alcalase, pancreatin, trypsin, pepsin (Novo Nordisk, Bagsvaerd, Denmark), diammonium salt, copper (II) sulfate, ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ferrous chloride, Ammonium heptamolybdate, DPPH (1,1-Diphenyl-2-picrylhydrazyl), Sodium phosphate tribasic, Pyridine and Pyrocatechol violet were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Potassium ferricyanide, trichloroacetic acid (TCA), sodium hydroxide, potassium persulphate and other antioxidant reagents were purchased from Merck (Germany). Alpha-deoxyribose was purchased from Fluka (Stockholm, Sweden). Other chemicals used were of analytical grade. ExtractionThe process of protein extraction was done for 1h by mixing algae powder (at a ratio of 1:10 w/v) in the distilled water solution comprising 0.3 M sodium chloride (after regulating the mixture pH to 10 with 1N NaOH). The attained dispersion was centrifuged (5,000 × g for 20 min) and the soluble proteins in the supernatant were precipitated after adjusting the pH to 4.20.5 M HCl. Lastly, the centrifuged mixture and the extracted proteins were freeze-dried (Feyzi et al., 2018). The Hydrolysis Using EnzymesThe hydrolysis by means of enzymes was done in proportion to the technique explained by Sarabandi et al. ( 2018) with minor amendments. Prepared samples were dissolved in the 0.01 M phosphate buffer saline at a ratio of 5% (w/v) for 30 min. Then, the enzymatic hydrolysis was carried out using alc...

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Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases

Abstract: * Correspondence: shihanw@jlau [...]

Cited by 10 publications

References 52 publications

Characterization of Yeast Protein Hydrolysate for Potential Application as a Feed Additive

Characterization of Yeast Protein Hydrolysate for Potential Application as a Feed Additive

Review on Extraction, Modification, and Synthesis of Natural Peptides and Their Beneficial Effects on Skin

Stabilization of Chlorella Bioactive Hydrolysates Within Biopolymeric Carriers: Techno-functional, Structural, and Biological Properties

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