Antioxidant activity of Artemisia (Artemisia annua) extract on several concentrations and solvents

Abstract

Artemisia contains secondary metabolites with antioxidants properties. The study aimed to obtain the type and concentration of effective solvents to produce Artemisia extract with maximum antioxidant activity. The research was conducted at the Laboratory of Indonesian Spices and Medicinal Crops Research Institute in 2015. The Artemisia used for the research came from Lembang, West Java. The study was arranged in a randomized block design, 7 treatments, and 2 replications. The treatment consisted of a combination of two solvents (ethanol and methanol) and three solvent concentrations (50, 70, and 96%) and water as control. Artemisia was processed into powder and then extracted following the treatments. The extract obtained was then tested for its antioxidant activity using the DPPH method. The type and concentration of solvent had a significant effect on the yield and antioxidant activity of Artemisia extract. The use of ethanol solvent resulted in higher extract yield and antioxidant activity than methanol. The higher the solvent concentration, the smaller the extract yield. The antioxidant activity of Artemisia ethanolic extract was stronger than methanolic extract. The IC50 value of the Artemisia ethanolic and methanolic extract were 20.61 ppm, and 25.06 ppm. The most effective solvent concentration to extract Artemisia was ethanol 70%.

Antibacterial and Phytochemical Screening of Artemisia Species

“…Since the values obtained in the present study are higher ( Table 1 ), we believe ethanol extraction to be more efficient than the solvents listed above. Similar results were recently communicated by Sembirin, who showed that the amount of antioxidant compounds extracted from A. annua L using an ethanol solvent was higher than the amount of antioxidant compounds extracted by a methanol solvent [ 42 ]. A higher value was also observed for the hydroalcoholic extract of A. absinthium L. from India, obtained from the aboveground parts of the plant (9.29 ± 0.51 mg GAE/g DW), as compared to hexane (0.43 ± 0.07 mg GAE/g DW) and methanol (3.55 ± 0.39 mg GAE/g DW) extracts [ 43 ].…”

Identification of Flavonoids in Artemisia annua L. by High-performance Liquid Chromatography and Evaluate the Antioxidant Activity

“…14 It has been widely used in china as a medical treatment as it contains secondary metabolic compounds that have antioxidant properties. 15,16 Which has attracted the attention of researchers and specialists as an effective drug against malaria. 17 It contained artemisinin and essential oil compounds.…”