Antioxidant Activity of Cashew Apple Dreg Extract and Their Effect in Traditional Processing of Coconut Oil

Abstract

ABSTRAKPenelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak ampas buah semu jambu mete dan kemampuannya sebagai antioksidan pada pengolahan minyak kelapa secara tradisional. Aktivitas antioksidan ditentukan melalui metode 2,2-difenil-1-pikrilhidrazil (DPPH). Penelitian ini menunjukkan seluruh fraksi ekstrak etanol mengandung senyawa flavonoid dan tanin/polifenol dan juga memiliki aktivitas antioksidan. Ekstrak etanol ampas buah semu jambu mete memiliki persen inhibisi tertinggi. Berbeda dengan fraksi n-heksan yang memiliki IC 50 terendah. Aplikasi pada pengolahan minyak kelapa tradisional menunjukkan penambahan ekstrak etanol sebesar 2,5 % menunjukkan hasil terbaik berdasarkan pada nilai angka peroksida dan bilangan TBA.Kata kunci : antioksidan, buah semu jambu mete, DPPH, minyak kelapa. ABSTRACTThe aim of this study was to determine antioxidant activity of cashew apple dreg extract and their antioxidant activity in traditional processing of coconut oil. Cashew apple dreg was extracted by ethanol and fractionation by DCM, n-hexane and water. The antioxidant activity from dreg extract was determined using 2,2-diphenyl-1-pycrylhydrazil (DPPH). The result showed that all ethanol fractions of cashew apple dreg contain flavonoid and tannins/polyphenols. Based on scavenging DPPH all of ethanol fractions showed antioxidant activity. The greatest DPPH inhibition was observed for ethanol extract. On the contrary, n-hexane fraction showed the lowest IC 50 . Application in traditional processing of coconut oil showed that the addition 2,5 % ethanol extract provide the best product according to the lower content of peroxide value and TBA number.