Antioxidant Activity of DPPH, CUPRAC, and FRAP Methods, as well as Activity of Alpha-Glucosidase Inhibiting Enzymes from Tinospora crispa (L.) Stem Ultrasonic Extract

Irawan, Candra; Putri, Imalia Dwi; Sukiman, Maman; Utami, Andita; Ismail, Ismail; Putri, Ratna Komala; Lisandi, Anisa; Pratama, Andrean Nur

doi:10.5530/pj.2022.14.128

Cited by 10 publications

(5 citation statements)

References 9 publications

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“…Ekstrak isolat M7 dan P1 memiliki aktivitas antioksidan yang berbeda yang diindikasikan oleh nilai IC50 yang bervariasi baik terhadap radikal DPPH maupun ABTS (Tabel 1). Berdasarkan nilai IC50-nya, aktivitas anitoksidan ekstrak bahan alam dapat digolongkan menjadi beberapa kategori, antara lain sangat kuat (<50 µg/mL), kuat (50-100 µg/mL), moderat (101-150 µg/mL), lemah (>150 µg/mL) (Irawan et al, 2022) Viabilitas sel MCF-7 menurun setelah diberi perlakuan ekstrak P1 dan M7 selama 48 jam. Ekstrak tersebut juga menginduksi terjadinya perubahan morfologi sel MCF-7 seperti adanya kerusakan membran dan apoptosis sel MCF-7, sehingga densitas MCF-7 yang diuji dengan ekstrak bakteri lebih rendah dibandingkan pada perlakuan tanpa ekstrak (Gambar 1).…”

Section: Aktivitas Antioksidan Ekstrak Bakteri Tanahunclassified

Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi

Priyanto,

Hening,

Permatasari

et al. 2024

J. I. Dasar

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Soil bacteria could be the promising source of secondary metabolites with diverse pharmacological activities. Bacteria coded as P1 and M7 were isolated from soil obtained from Muna Island, Southeast Sulawesi. This research aimed to evaluate the antioxidant activity, cytotoxic property, and identify the chemical profile of the extract derived from soil bacteria P1 and M7. Based on 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, extract derived from P1 isolate was moderately active against ABTS free radical with IC50 of 126.22±10.82 µg/mL, and less active on DPPH (IC50: 721.95±16.59 µg/mL), while M7 extract was less active towards DPPH and ABTS. Interestingly, these extracts in the concentration of 100 µg/mL were cytotoxic to breast cancer cell (MCF-7), as tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. MCF-7 viability was decreased up to 69.83±1.46 % and 61.49±0.76 % after treated with P1 and M7 extract, respectively. The extracts were also induced membrane disruption and apoptosis of MCF-7 cells. The extracts contained phenol (9.52±0.22 and 10.41±0.09 mg EAG/g extract, for P1 and M7, respectively) and flavonoids (9.16±0.16 and 9.29±0.24 mg EQ/g extract, for P1 and M7, respectively). According to Gas Chromatography Mass Spectrometry (GC-MS) analysis, Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) that previously well known as antioxidant and cytotoxic compounds, were found in the M7 and P1 extract. This compound may play a vital role in the radical scavenging and cytotoxic properties of P1 and M7 extracts. The results of this study indicate that P1 and M7 extracts could be the potential source of antioxidant and cytotoxic compounds and need to be further developed for pharmaceutical use.

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Section: Aktivitas Antioksidan Ekstrak Bakteri Tanahunclassified

Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi

Priyanto,

Hening,

Permatasari

et al. 2024

J. I. Dasar

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“…D2 extract exhibited antioxidant activity against two free radicals, including DPPH (IC50: 242.61±3.2 µg/mL) and ABTS (IC50: 317.32±9.3 µg/mL) (Table 4). According to its IC50 values (>150 µg/mL), D2 extract has weak antioxidant activity [19]. However, the antioxidant of D2 extract (extract derived from leaf endophytic bacteria from M. fatua) is still more active than leaf extract of the plant extracted with various organic solvents, which is ranging from 535-2,630 µg/mL [6].…”

Section: Antioxidant and Cytotoxic Properties Of D2 Extractmentioning

confidence: 99%

Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt

Agung PRIYANTO,

PRIMAHANA,

PRASTYA

et al. 2024

jrp

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Endophytic bacteria isolated from medicinal plants are recognized as a source of valuable secondary metabolites. The present study aimed to isolate and evaluate the potential antibacterial, antioxidant, and cytotoxic properties of endophytic bacteria associated with Myristica fatua Houtt, along with molecular identification and chemical composition analysis of the most potential isolate. A total of 25 isolates were obtained from the root, leaf, and stem bark of M. fatua. 16 isolates (64%) exhibited antibacterial activity against at least one target strain. Preliminary screening showed that the D2 isolate displayed remarkable antibacterial activity against four targeted strains. D2derived metabolite extract possessed moderate antibacterial activity with minimum inhibitory concentration (MIC) of 1,875 µg/mL for E. coli, P. aeruginosa, and S. aureus, and 3,750 µg/mL for B. subtilis, and minimum bactericidal concentration (MBC) of > 3,750 µg/mL. Furthermore, D2 extract exhibited weak antioxidant activity against DPPH (IC50: 242.61±3.2 µg/mL) and ABTS (IC50: 317.32±9.3 µg/mL). In the concentration of 100 µg/mL, the extract was able to decrease the viability of MCF-7 and HepG2 cells, with inhibition percentages of 52.6±9.7 and 41.5±9.36%, respectively. 16S rRNA-based identification confirmed D2 isolate was very similar (99.92%) to Stutzerimonas stutzeri strain SM12. The total phenolic content (TPC) and total flavonoid content (TFC) of the D2 extract were 21.11±2.3 mg GAE/g extract and 8.12±1.2 mg QE/g extract, respectively. At least fifteen volatile compounds were found in the D2 extract, as identified using GC-MS analysis. Four out of 15 compounds are well known responsible for antimicrobial, antioxidant, and cytotoxic properties, such as 2,4-di-tert-butyl phenol, pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), and bis(2-ethylhexyl) phthalate. The present study suggests that endophytic S. stutzeri strain D2 isolated from M. fatua Houtt could be a good source of active compounds valuable for pharmaceutical purposes.

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“…The standard curves of ascorbic acid (4 -20 mg/L) and Trolox (4 -12 mg/L) were used to determine the antioxidant capacity of the sample solution. FRAP was calculated against the dried extract [19,20].…”

Section: Antioxidant Capacity Assaymentioning

confidence: 99%

“…The standard curve of ascorbic acid (12 -40 mg/L) and Trolox (4 -12 mg/L) was used to determine the antioxidant capacity of the sample solution. CUPRAC was calculated against the dried extract [19,20]. The extraction yield indicated that the hot maceration had the maximum extraction efficiency (Tabel 1).…”

Section: Antioxidant Capacity Assaymentioning

confidence: 99%

The Effect of Various Extraction Methods and Solvents on the Phytochemical Contents and Antioxidant Capacities of Safflower Florets (Carthamus Tinctorius L.) from South Sulawesi

Satrianegara,

Tahar,

Rukmana

et al. 2024

Trends Sci

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Safflower is a traditional medicine commonly applied for the treatment of varicella in South Sulawesi. The aim of this study is to study the effect of extraction methods and solvents on phytochemical content and antioxidant capacity in the extraction of safflower. The study began with sample extraction with various solvents, i.e., water, ethanol 70, methanol, ethyl acetate and hexane and various methods, i.e., reflux, Soxhlet, hot maceration, cold maceration and room temperature maceration. Each extract was standardized for its phytochemical content, i.e., phenolics, flavonoids and carotenoids and the antioxidant capacities were also determined by the radical scavenging 2,2-diphenyl-1-picrylhydrazyl, total antioxidant capacity, cupric ion reducing antioxidant capacity and Ferric reducing antioxidant power. The results indicated that the optimum total phenolic content was obtained when the florets were refluxed with methanol, flavonoids were refluxed with 70 % ethanol and carotenoids were Soxhleted with methanol. The research data also showed that 70 % ethanol and methanol have maximal antioxidant capacity. The results conclude that 70 % ethanol and methanol as solvents have the potential to extract the highest levels of phytochemical content and antioxidant substances. HIGHLIGHTS The phytochemical content and antioxidant capacity of safflower floret extract are influenced by the selection of solvent and extraction method. Polar solvents (70 % ethanol or methanol) can efficiently extract higher levels of phytochemical components and antioxidant substances from safflower florets. The hot extraction approach exhibits a higher potential for extracting phytochemical content compared to the cold extraction method, but its antioxidant capacity exhibits the opposite. The application of cold maceration, combined with methanol or 70 % ethanol as solvents, has been identified as a promising method for the extraction of phytochemical compounds and antioxidant substances. GRAPHICAL ABSTRACT

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Antioxidant Activity of DPPH, CUPRAC, and FRAP Methods, as well as Activity of Alpha-Glucosidase Inhibiting Enzymes from Tinospora crispa (L.) Stem Ultrasonic Extract

Cited by 10 publications

References 9 publications

Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi

Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi

Bioactivity potential and chemical profile of endophytic Stutzerimonas stutzeri strain D2 isolated from Myristica fatua Houtt

The Effect of Various Extraction Methods and Solvents on the Phytochemical Contents and Antioxidant Capacities of Safflower Florets (Carthamus Tinctorius L.) from South Sulawesi

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