ANTIOXIDANT ACTIVITY OF EXTRACTS OF MALLOTUS PHILIPPINENSIS FRUIT AND BARK

Abstract

Several extracts from Mallotus philippinensis fruits and bark were prepared and evaluated for their total antioxidant activity (TAA), antiradical activity against DPPH· (2,2‐diphenyl‐1‐picrylhydrazyl radical) and reducing power. The total phenolics and tannin contents in extracts were determined. The extract of the bark showed the strongest antiradical activity and reduction power; its TAA was 5.27 mmol Trolox equivalents/g. The TAA of other extracts ranged from 0.05 to 1.79 mmol Trolox equivalents/g extract. The content of total phenolics in the bark extract was 541 mg/g. The content of tannins in MP‐4, expressed as absorbance units per mg at 500 nm, was found to be 0.412. Several phenolic constituents in the extracts were detected by reverse‐phase high‐performance liquid chromatography using a gradient solvent system with UV‐DAD detection. Acetone was a suitable solvent for extraction of natural antioxidants from M. philippinensis fruits. PRACTICAL APPLICATIONS An acetonic and methanolic extraction of Mallotus philippinensis fruit and bark affords extracts that have been characterized as possessing strong antioxidant activities; this may open new avenues for the utilization of these extract as nutraceuticals. The biological activity of compounds present in the extracts has been well documented in many studies published in scientific journals. We want to emphasize that a procyanidin‐rich extract from pine (Pinus maritime) bark was patented under the trade name of Pycnogenol (PYC) and is utilized throughout the world as a nutritional supplement and as a phytochemical remedy for various diseases ranging from chronic inflammation to circulatory dysfunction (Packer et al. 1999).

Antioxidant activity of phenolic fractions of Mallotus philippinensis bark extract

“…The values of TAA in fractions III-VI were several times higher than those reported in the extracts of phenolic compounds or their fractions separated from tannin-rich plant materials such as leguminous seeds, almonds, and hazelnuts (Amarowicz et al 2004a(Amarowicz et al , 2005Alasalvar et al 2006). In our previous study (Arfan et al 2007), the extract obtained from the Mallotus philippinensis fruit using acetone exhibited the total antioxidant capacity of 1.54 mmol Trolox/g. The fractions separated from the Mallotus philippinensis bark crude extract were investigated for the antiradical activity and reducing power.…”

“…Mallotus philippinensis Muell. (Euphorbiaceae), also known as kamala or kamopollaka, was used as a source of a yellow dye (kamala dye), and as an antioxidant for ghee and vegetable oils (Rao & Seshadri 1947) In our previous study (Arfan et al 2007), several extracts of Mallotus philippinensis fruits and bark were prepared and evaluated for their total antioxidant activity, antiradical activity against DPPH…”

Medicinal plants in the treatment ofHelicobacter pyloriinfections

“…(Euphorbiaceae) has long been used in the Chinese, Ayurvedic (Indian), Arabic, and Unani traditional medicine systems as an anthelminthic in man and animals (Jost et al, 1996). Other medicinal properties ascribed to this plant include cathartic, aphrodisiac, antifungal; antibacterial, antiparasitic, antioxidant (Akhtar & Ahmad, 1992;Arfan et al, 2007;Kumar et al, 2006;Hussain et al, 2008). Zaidi et al (2009) evaluated the anti-H. pylori activity of 50 commonly used traditional Pakistan medicines extensively utilized for the treatment of gastrointestinal disorders.…”

Development of Nanodrug for Treatment of Breast Cancer Using Mallotus Tetracoccus Leaves - Standardisation, Synthesis and Characterisation

“…Several species of the genus Mallotus are a rich source of biologically active compounds such as phloroglucinols, tannins, terpenoids, coumarins, benzopyrans and chalcones [1,2]. The reported bioactivities of the extracts or the individual chemical constituents isolated from this genus include antipyretic [3], anti-inflammatory, hepatoprotective [4], antioxidant and radical scavenging activities [5].…”