Antioxidant Activity of Flesh and Skin of Eptatretus Burgeri (Hag Fish) and Enedrias Nebulosus (White Spotted Eel)

Abstract

Ethyl acetate and diethyl ether extracts, previously extracted with methanol, were obtained from flesh and skin of Eptatretus burgeri (hag fish) and Enedrias nebulosus (white spotted eel). Eight different extracts (4 mg/mL) were tested for DPPH free radical scavenging activity and the values were compared with commercial antioxidants (α-tocopherol and BHT). All extracts of E. burgeri exhibited significantly positive results (> 65%) in scavenging DPPH radicals compared to E. nebulosus. Thus, only E. burgeri extracts were tested for dose effect, hydrogen peroxide, hydroxyl radical, superoxide scavenging assays and heat stability at 25, 50, 75 and 100 °C for 30 min. All extracts of E. burgeri showed higher DPPH radical scavenging activities by increasing concentration. Significantly higher results were observed for hydroxyl radical scavenging activity when compared with commercial antioxidants. Relatively moderate activity and very low activity were exhibited for superoxide scavenging and hydrogen peroxide activities, respectively. Diethyl ether extracts of E. burgeri were stable with increased temperature, while ethyl acetate extracts were stable up to 75 °C. Thus, there is a high potential for E. burgeri being rich with heat-stable antioxidants that can scavenge hydroxyl radicals.

Antibacterial and antioxidant properties of crude extract, fractions and compounds from the stem bark of Polyscias fulva Hiern (Araliaceae)

“…The method of Ekanayake et al [13] was used in this test. The method is based on the inhibition of the auto-oxidation of pyrogallol by phenolic compounds.…”

Epiphytic marine pigmented bacteria: A prospective source of natural antioxidants

“…The extracts were evaporated and concentrated with rotary evaporator (Equitron, Roteva (63) R-V) until complete dryness and finally retained in minimum HPLC grade methanol. 0.5–1.0 mL of sample was taken in a pre-weighed aluminium foil and allowed to dry for weight determination ( Ekanayake et al , 2004 ). The known concentrations of sample viz.…”

In Vitro Antioxidant Activity of Aqueous Ethanol Extract of Flacourtia Indica Merr.

“…As the odd electron of the radical becomes paired off in the presence of a hydrogen donor that is a free radical scavenging antioxidant the absorption strength decreases and the resulting decolorization is stiochiometric with respect to the number of electrons captured 26 . This reaction has been widely used to test the ability of compounds to act as free radical scavengers or hydrogen donors and to evaluate the anti-oxidative activity of foods and plant extracts [27][28][29][30] . The extract showed to possess DPPH radical scavenging ability as compared to the standard.…”