ANTIOXIDANT ACTIVITY OFINONOTUS OBLIQUUSGROWN ON GERMINATED BROWN RICE EXTRACTS

Abstract

The aim of this study was to determine the antioxidant activity of different extracts from Inonotus obliquus (IO) grown on germinated brown rice by employing several established methods. The total phenolic and flavonoid contents were performed by Folin–Ciocalteau and aluminum chloride colorimetric assay, respectively. All the extracts showed strong antioxidant activity. However, the ethanol‐based extract showed significantly highest antioxidant activity with the values of the half maximal inhibitory concentration (IC50) for 1,1‐diphenyl‐2‐picryl‐hydrazyl (DPPH), 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS), hydroxyl and superoxide radical scavenging activities were 16, 30, 45, and 100 µg/mL, respectively. In addition, the antioxidant activities of IO extracts were highly correlated with the observed total phenolic and flavonoid contents. Our study successfully demonstrates that the extracts from IO could be a potential source of antioxidant as a medicinal and dietary supplement. PRACTICAL APPLICATIONS Inonotus obliquus (IO) has been used as a medicine in many Asian countries. IO extracts build up immunity, treat influenza and other viral and bacterial infections, and improves skin elasticity. As a folk remedy, IO was taken in tea or powder form. Japanese scientists reported that topical use of IO helps combat the signs of aging. In Japan, it is used in creams to treat wrinkles. The water extract of IO has antitumor and antimicrobial activities. In addition, hot water extract from IO inhibits the cellular proliferation in cancer cell lines in human stomach. IO is regarded as a “functional food” and believed to promote longevity.

Anti-Oxidant and Anti-Inflammatory Activities of Inonotus obliquus and Germinated Brown Rice Extracts

“…Our findings show that the bio-activity of Inonotus obliquus was not reduced or changed when it was grown on germinated brown rice. The higher activity of IOGBR may be related to its higher phenolic and flavonoid contents [17]. Park et al [13] reported that Phellinus linteus grown on brown rice contains greater amounts of some constituents including ergosterol peroxide, γ-aminobutyric acid (GABA), and β-glucan compared to Phellinus linteus .…”

“…The SOD-like activities of samples were determined using the method described by [17]. Briefly, different samples (200 μL) were mixed with pyrogallol solution (200 μL, 7.2 mM in water) and 50 mM Tris-HCl buffer at pH 8.5 containing 10 mM EDTA (3 mL).…”

“…The mushroom grown on germinated brown rice have been found to have an antioxidative role by suppressing the oxidative liver damage and stopping the overproduction of immunoglobulin (Ig) [12,15,16]. In our previous study, we reported the antioxidant activity of extracts of different polarities from IO grown on germinated brown rice (IOGBR); the ethanol extracts showed the highest activity among the three extracts [17]. It is very important to compare the bioactivity of IOGBR with that for GBR, IO.…”

Chemical characterization and biological activity of Chaga (Inonotus obliquus), a medicinal “mushroom”

“…The phenolic acids found were gallic, protocatechuic and p-hydroxybenzoic acids, as also the related compound cinnamic acid ( Table 1) Regarding the antioxidant potential ( (Pereira et al, 2012). In previous study of antioxidant activity of mycelia of I. obliquus (Debnath et al, 2013), the scavenging activity of water, ethanol and methanol extracts on DPPH's were determined, and it was concentration dependent. The scavenging activities were ranged from 12.50-200 mg/mL, respectively.…”

Antioxidant and anti-inflammatory activity of Polygonatum sibiricum rhizome extracts

“…This study was therefore conducted to evaluate the antioxidant and antiinflammatory activity of water extracts of P. sibiricum. Fresh P. sibiricum rhizome (300 g) was extracted with distilled water (DW) (100 g/700 mL) at 85 °C for 3 h [12] . The The stock solution was prepared by dissolving the extracted mass into DW for different assays.…”