Antioxidant Activity of Indian Propolis - An<i> In Vitro </i>Evaluation

Abstract

Antioxidants from the natural products are essential to prevent the progression of free radical mediated diseases. In the present study, ethanol extract of propolis collected from different geographical origin were evaluated for their free radical scavenging potential by employing different in-vitro assays such as DPPH, ABTS, Nitric oxide and Hydrogen peroxide. All the tested samples contained considerable amount of total phenols and vitamin C content. In the entire assay, the percentage of inhibition increased with the increase in concentration. Among the propolis samples collected, the highest activity was found in Tamil Nadu, Kerala, Karnataka and Haryana. The difference in the antioxidant activity level was obtained from the assay may reflect a relative difference in the ability of antioxidant compounds to scavenge different free radicals in the extract. Phenols and vitamin C are the major contributors to antioxidant activity in propolis. The propolis from these locations may be of considerable interest in preventing the ill effects of excessive free radical generation in the human body.

Identification of Resveratrol as Bioactive Compound of Propolis from Western Romania and Characterization of Phenolic Profile and Antioxidant Activity of Ethanolic Extracts

“…Belfar et al reported a stronger antioxidant activity for 4 methanolic extracts of Algerian propolis with IC 50 varying from 0.007 to 0.066 mg/mL which was lower as compared to the value (0.184 mg/mL) for ascorbic acid used as control [37]. For 10 ethanolic extracts of Indian propolis, IC 50 varied between 0.33348 mg/mL and 0.60088 mg/mL, while for ascorbic acid was 0.28492 mg/mL [38]. Wang et al reported IC 50 values from 0.043 to 0.269 mg/mL for 20 samples of Korean propolis [22].…”

Extraction, Phenolic Content and Hydrogen Peroxide Scavenging Capacity of Extracts from Some Honey Samples, Propolis and Bee Pollen

“…Poor scavenging activity is observed with extracts from propolis (35.116 µg/mL) and bee pollen (38.383 µg/mL). In similar studies, the H2O2 scavenging assay of phenolic compounds extracted from propolis by various solvents (distilled water, absolute methanol, 50% methanol, and 70% methanol) presented different action on H2O2, which IC50 values were between 11.72 µg/mL and 765.75 µg/mL (Farooq Wali et al, 2016;Ramnath et al, 2016;Kızılpınar Temizer et al, 2017). Although these two bee products are quantitatively rich in phenolic compounds, but their extracts have a low antioxidant capacity compared to honey extracts, which suggests that the antioxidant capacity is not always dependent on the amount of phenolics and flavonoids, honeys are therefore a good source of antioxidant substances.…”

Effect of microwave and ultrasound‐assisted extraction methods on phytochemical extraction of bee propolis of Indian origin and its antibacterial activity

“…JKP had the highest % DPPH scavenging activity compared to RP. The antioxidant activity of propolis might be due to the presence of phenolic and flavonoids compounds (Ramnath & Venkataramegowda, 2016).…”