Antioxidant activity of liquid cultured Inonotus obliquus polyphenols using tween-20 as a stimulatory agent: Correlation of the activity and the phenolic profiles

Xu, Xin; Zhao, Wei; Shen, Meng-Wei

doi:10.1016/j.jtice.2016.10.011

Cited by 8 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was hypothesized that some phenolic compounds like phenolic acids might be the lignin‐derived phenols, 6,7 but polyphenols including flavonoids were probably produced by the mushroom via bioconversion of the lignin degradation products under oxidative stress during WS degradation 6,8,9 . We also found that Tween 20 as a stimulatory agent added to I. obliquus under submerged fermentation could significantly enhance the antioxidant phenolic compound production 10,11 . However, how surfactants affect the degradation of WS lignocellulose and the cooperative effects of surfactant and lignocellulose degradation on the production of phenolic compounds need more thorough studies to give better explanation.…”

Section: Introductionmentioning

confidence: 83%

“…The content of total phenolic compounds was determined using the Folin–Ciocalteu reagent method and calculated as gallic acid equivalents (GAE) 11,16 . The mycelia were washed with deionized water three times, dried to constant weight and ground into powder.…”

Section: Methodsmentioning

confidence: 99%

“…The column was a Kromasil C18 (250 × 4.6 mm, 5 μm) and the solvent system consisted of 0.1% aqueous formic acid and acetonitrile (95:5, 0 min; 90:10, 20 min; 5:95, 40 min). By matching the retention time with external standards, the phenolic components were identified and quantified as gallic acid, ferulic acid, ECG, EGCG, naringin, rutin, naringenin, phelligridin G, inoscavin B and davallialectone 11 …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Production of phenolic compounds and antioxidant activity via bioconversion of wheat straw by Inonotus obliquus under submerged fermentation with the aid of a surfactant

et al. 2020

Self Cite

View full text Add to dashboard Cite

BACKGROUNDThis study investigated the effect of surfactants on wheat straw biodegradation and the growth‐associated generation of exo‐ and endo‐phenolic compounds (EPC and IPC) and antioxidant activity expression by liquid‐cultured Inonotus obliquus, an edible and medicinal mushroom, also known as a white rot fungus. Changes in the chemical composition and multiscale structure of wheat straw, in the production and activity of EPC and IPC and in individual flavonoids were analyzed.RESULTSFungal pretreatment decreased significantly the contents of all lignocellulose components, increased and enlarged substrate porosity and caused changes in the structure of wheat straw with the aid of Triton X‐100. A gradual increase in EPC and IPC production was observed up to 6.4‐ and 1.5‐fold for 9 days. The EPC obtained on day 9 showed the highest antioxidant activity (IC50 of 30.96 mg L−1) against 2,2‐diphenyl‐1‐picrylhydrazyl radicals. High‐performance liquid chromatographic results indicated the presence of high amounts of epicatechin‐3‐gallate (ECG; (374.9 mg g−1) and epigallocatechin‐3‐gallate (EGCG; 447.2 mg g−1) in the EPC; other polyphenols were also enhanced but to a lesser extent. Surfactant supplementation was effective in enhancing flavonoid production and in increasing antioxidant activity in EPC.CONCLUSIONSThe results indicated enhanced accumulation of phenolic compounds, particularly ECG and EGCG in Inonotus obliquus via biodegradation and bioconversion of lignocellulose residues. They also indicated enhancement in the production of several flavonoids and also an increase in antioxidant activity in the product by a surfactant‐treated process, which may be a useful way of exploiting underused lignocellulosic residues to various high‐added‐value functional ingredients. © 2020 Society of Chemical Industry

show abstract

Section: Introductionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Production of phenolic compounds and antioxidant activity via bioconversion of wheat straw by Inonotus obliquus under submerged fermentation with the aid of a surfactant

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…It indicated birch extract contained other nutrients, which made the synthesis of polysaccharides containing galactose and xylose and needed further research. Based on the previous reports, the α-glucosidase inhibitory activity of polysaccharides might be attributed to many factors, including other molecular weight and structure [9,24].…”

Section: Discussionmentioning

confidence: 99%

“…After 13 days the fermentation ended up, the mycelial biomass was separated from fermentation broth by filtration, washed with deionized water until colorless, dried in the oven at 60 °C and weighed to obtain the mycelial biomass [24].…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation

et al. 2019

View full text Add to dashboard Cite

This study aimed to determine the effect of applying stimulatory agents to liquid cultured Inonotus obliquus on the simultaneous accumulation of exo-polysaccharides (EPS) and their monosaccharide composition. Different stimulatory agents (VB6, VB1, betulin and birch extract) were investigated for their effects on active exo-polysaccharides by submerged fermentation of I. obliquus. The mycelial biomass, reducing sugar content, EPS yield and α-glucosidase inhibition rate were determined, and the EPS obtained was analyzed for monosaccharide composition. The results showed that the addition of all the four stimulatory agents could significantly increase the inhibitory activity against α-glucosidase of EPS than the control, whereas EPS from 4 μg/mL VB1-containing medium had the best effect with an estimated IC50 value 24.34 μg/mL. Among the four stimulatory agents, VB6 gave maximum production of mycelial biomass and EPS at the concentration of 4 μg/mL with a increase of 50.79% and 114.46%, respectively. In addition, betulin had a significant effect on increasing the EPS yield and activity, and birch extract had a significantly stimulatory effect on the mycelial growth and the polysaccharides activity, only slightly worse than VB6 and VB1. Moreover, the addition of different stimulatory agents changed the monosaccharide composition of polysaccharides, which had a correlation with polysaccharide activity.

show abstract

Making Use of Genomic Information to Explore the Biotechnological Potential of Medicinal Mushrooms

Kües

Badalyan

2017

Medicinal and Aromatic Plants of the World

View full text Add to dashboard Cite

Antioxidant activity of liquid cultured Inonotus obliquus polyphenols using tween-20 as a stimulatory agent: Correlation of the activity and the phenolic profiles

Cited by 8 publications

References 30 publications

Production of phenolic compounds and antioxidant activity via bioconversion of wheat straw by Inonotus obliquus under submerged fermentation with the aid of a surfactant

Production of phenolic compounds and antioxidant activity via bioconversion of wheat straw by Inonotus obliquus under submerged fermentation with the aid of a surfactant

Simultaneous Use of Stimulatory Agents to Enhance the Production and Hypoglycaemic Activity of Polysaccharides from Inonotus obliquus by Submerged Fermentation

Making Use of Genomic Information to Explore the Biotechnological Potential of Medicinal Mushrooms

Contact Info

Product

Resources

About