Antioxidant activity of methanol extracts from<i>Ganoderma lucidum</i>Karst. Mycelia

Darsih, Cici; Indrianingsih, Anastasia Wheni; Apriyana, Wuri; Hayati, Septi Nur; Rosyida, Vita Taufika; Nisa, Khoirun; Ratih, Dwi; Indirayati, N

doi:10.1088/1755-1315/251/1/012015

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…At the same time, the effect of the TM28 filtrate on hyphae of Fp growth under liquid culture conditions was analyzed. One milliliter of Fp spore suspension (10 7 CFU mL −1 ) was added to a conical flask with 50 mL PDB medium, cultured at 28°C with shaking at 150 rpm for 12 h, 2.5 mL of TM28 fermentation filtrate was added and cultured for another 48 h. The hyphae of Fp were collected in a suction flask and dried in an oven at 65°C until constant weight ( Darsih et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Antagonistic effects of Talaromyces muroii TM28 against Fusarium crown rot of wheat caused by Fusarium pseudograminearum

Yang,

Cui,

Wei

et al. 2024

Front. Microbiol.

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Fusarium crown rot (FCR) caused by Fusarium pseudograminearum is a serious threat to wheat production worldwide. This study aimed to assess the effects of Talaromyces muroii strain TM28 isolated from root of Panax quinquefolius against F. pseudograminearum. The strain of TM28 inhibited mycelial growth of F. pseudograminearum by 87.8% at 72 h, its cell free fermentation filtrate had a strong antagonistic effect on mycelial growth and conidial germination of F. pseudograminearum by destroying the integrity of the cell membrane. In the greenhouse, TM28 significantly increased wheat fresh weight and height in the presence of pathogen Fp, it enhanced the antioxidant defense activity and ameliorated the negative effects of F. pseudograminearum, including disease severity and pathogen abundance in the rhizosphere soil, root and stem base of wheat. RNA-seq of F. pseudograminearum under TM28 antagonistic revealed 2,823 differentially expressed genes (DEGs). Most DEGs related to cell wall and cell membrane synthesis were significantly downregulated, the culture filtrate of TM28 affected the pathways of fatty acid synthesis, steroid synthesis, glycolysis, and the citrate acid cycle. T. muroii TM28 appears to have significant potential in controlling wheat Fusarium crown rot caused by F. pseudograminearum.

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Section: Methodsmentioning

confidence: 99%

Antagonistic effects of Talaromyces muroii TM28 against Fusarium crown rot of wheat caused by Fusarium pseudograminearum

Yang,

Cui,

Wei

et al. 2024

Front. Microbiol.

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“…The antioxidant activity of G. mangostana peel extract was performed by DPPH method according to Darsih et al (2019) with light modification [6]. Several concentrations of G. mangostana ethanolic extracts were dissolved in methanol, and then reacted with DPPH 1.01 mM at dark room temperature for 30 minutes.…”

Section: Dpph Scavenging Activity Assaymentioning

confidence: 99%

In Vitro Study of Antioxidant and Antimicrobial Activities of Garcinia mangostana L. Peel Extract

Indrianingsih¹,

Rosyida²,

Ratih³

et al. 2020

Proceedings of the 5th International Conference on Food, Agriculture and Natural Resources (FANRes 2019)

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Plant extract are natural additives that are in great demand. Many biological capabilities of plant extracts in the fields of health and medicine, make research on plant extract quite rapid. Mangosteen (Garcinia mangostana L.) is one of the most famous fruits in Indonesia. In this paper, the antimicrobial and antioxidant activities of mangosteen peel were studied. The mangosteen peel extract were prepared by maceration method using ethanol for 48 hours. After the evaporation, the crude extracts were tested using DPPH assay for antioxidant activity and antibacterial activity was performed using dilution method. The scavenging activity of mangosteen peel extracts values in the range of 73.57-79.14% with extract concentration of 100 ppm to 800 ppm, respectively. The antibacterial activity of mangosteen peel extract were conducted against Gram-positive bacteria (S. aureus) and Gram-negative bacteria (E. coli). The inhibition zone of mangosteen peel extract was 6.95 mm against S. aureus and 5.33 ppm against E. coli at extract concentration of 10000 ppm. The results obtained indicate that mangosteen peel extract is potentially applied in the fields of medicine and health.

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Antioxidant activity of methanol extracts fromGanoderma lucidumKarst. Mycelia

Cited by 2 publications

References 18 publications

Antagonistic effects of Talaromyces muroii TM28 against Fusarium crown rot of wheat caused by Fusarium pseudograminearum

Antagonistic effects of Talaromyces muroii TM28 against Fusarium crown rot of wheat caused by Fusarium pseudograminearum

In Vitro Study of Antioxidant and Antimicrobial Activities of Garcinia mangostana L. Peel Extract

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