Antioxidant Activity of Nipah Endophytic Fungi (Nypha fruticans Wurmb) from Tanjung Jabung Timur Jambi

Abstract

Nipah or Nypa fruticans Wurmb is a member of the Palmae tribe that lives in the Mangrove ecosystem and is known to have rich bioactive sources, one of which is antioxidants. The ability of N. fruticans as an antioxidant is also possible for endophytes associated with N. fruticans. This study aims to obtain endophytes that live in N. fruticans leaf tissue and find out their ability as antioxidant agents using synthetic free radicals 2.2 diphenil-1-pikrilhidrazil (DPPH). Samples taken at Nipah Panjang Tanjung Jabung Timur, Isolation and production were carried out at the Jambi University Faculty of Science and Technology Engineering and Biotechnology Laboratory, absorbance measurements using UV-VIS at λ 517 nm at the Jambi University CRC. The antioxidant activity of isolates was shown from the value of Inhibitor Concentration (IC50) which caused 50% loss of DPPH free radicals. From the results it was obtained that the six isolates (DN01, DN02, DN02, DN03, DN04, DN05, and DN06) value the antioxidant activity of endophytic extracts lower than vitamin C activity and still classified as weak. Even though endophytic Nypa fruticans are very weak, N. fruticans endophytic in all treatment concentrations still have the ability as antioxidants and optimization methods are needed to get good antioxidant values.
 
 Keywords: Mangrove, Nypa fruticans Wurmb, endophytes, antioxidants, DPPH

Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa

“…In a previous work, the flavonoid kaempferol found within F. religiosa scavenged OH radicals [42]. Numerous extractions of plants and flavonoids, such as mangiferin and naringin, have also been shown to scavenge hydroxyl free radicals in a content-dependent approach [35][36][37]40,45]. Numerous flavonoids generated as secondary metabolites from various plants have previously been demonstrated to scavenge OH radicals [50,51].…”

“…Once, reduced and transformed into DPPH-H, the DPPH radical has a dark purple hue in solution, but when reduced as well as transformed into DPPH-H, it turns colorless or light yellow [35]. In vitro, several extractions of plants have been shown to neutralize DPPH radical scavenging activity [36][37][38][39][40]. DPPH radicals were scavenged by various extracts of F. religiosa in a content-dependent approach.…”

“…•− . Naringin, mangiferin, myricetin, quercetin, and rutin, among other extractions of plants and flavonoids, have been discovered to scavenge superoxide free radicals in a content-dependent approach [35][36][37]40,45,54]. Various in vitro approaches may be used to measure the antioxidant properties (total antioxidant potential) of plants as well as plant products.…”

Green synthesis of MgO nanoparticles using Moringa oleifera leaf aqueous extract for antibacterial activity

“…Antioxidant activity of the M. Oleifera leaf aqueous extract measured by DPPH method is shown in Table 1. A testing anti-free radical activity using DPPH is a must in every test of antioxidant and phytochemical ability of a plant extract [20][21][22]. The antioxidant activity of M. oleifera leaf aqueous extract displays a good DPPH inhibition with 64.60±0.69% RSA (radical scavenging activity) as compared ascorbic acid showed around 71.72±0.56% inhibition of DPPH.…”

“…No significant characteristic peaks appear from Mg or other impurities detected on the diffractogram indicating the high purity of the synthesized MgO nanoparticles. The average diameter of crystalline (D) was measured using the Scherrer's formula (Equation 2) for the (200) plane obtained at 21.07 nm [20,21].…”