Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro

Abstract

The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the TUNEL Test. The use of antioxidants during cultivation did not increase (P> 0.05) the final blastocyst production rate. The treatments T2, T3, T4 and T5 had the lowest (P< 0.05) apoptotic indexes (4.5±1.1%, 8.4±2.5%, 3.4±1.1% and 5.5±0.9%, respectively) when compared to T1 treatment (10.0±1.4%). The number of inner cell mass did not differ (P> 0.05) among embryos from different treatments. The addition of antioxidant obtained from oily extract of Lippia origanoides reduces the apoptosis rate and improves the quality without increasing the total in vitro production of bovine embryos.

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

“…Other factors that influence this are culture media, changes in pH, osmolarity, temperature, exposure to light, concentrations of CO 2 and oxygen, humidity, manipulation of cells, and cryopreservation methods [20,21]. If the conditions are not adequate, they will generate stress to the oocyte by affecting the cytoplasm and cell membrane, causing changes in nucleic acids, lipids, and proteins [22,23].…”

Produção in Vitro De Embriões E Novas Estratégias Na Produção De Oócitos Em Bovinos

“…É utilizado um sistema de bomba a vácuo conectado à agulha para a colheita dos oócitos e do líquido folicular em um tubo coletor, que posteriormente será encaminhado ao laboratório para produção in vitro de embriões. Os folículos aspirados devem ter diâmetro entre 2-8 mm, folículos menores que 2 mm são incapazes de recomeçar a fase de meiose e os maiores que 8 mm são desprezados, pois normalmente se encontram em estágio de atresia, inviabilizando o processo de maturação in vitro (MIV) (GONÇALVES et al, 2008;SOLLECITO et al, 2019).…”