Antioxidant activity of oleuropein and semisynthetic acetyl-derivatives determined by measuring malondialdehyde in rat brain

Rizzo, Milena; Ventrice, Domenica; Giannetto, Francesca; Cirinnà, Salvatore; Santagati, Natale Alfredo; Procopio, Antonio; Mollace, Vincenzo; Muscoli, Carolina

doi:10.1111/jphp.12807

Cited by 32 publications

(17 citation statements)

References 21 publications

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“…Furthermore, the toxicity of pre-formed fibrils treated with the polyphenol was not significantly affected even after a prolonged treatment (5 days), yet in the presence of a significant reduction of oxidative stress. The reduced cellular levels of ROS could result from the radical scavenging properties of OleA in accordance with previous studies describing the role of the antioxidant properties of this polyphenol in the prevention of amyloid assembly cytotoxicity 70 , 71 . However, in this case, aggregate toxicity persisted because OleA failed to reduce the membrane activity of the pre-formed fibrils treated with the polyphenol.…”

Section: Discussionsupporting

confidence: 91%

Oleuropein aglycone stabilizes the monomeric α-synuclein and favours the growth of non-toxic aggregates

Palazzi

Bruzzone

Bisello

et al. 2018

Sci Rep

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α-synuclein plays a key role in the pathogenesis of Parkinson’s disease (PD); its deposits are found as amyloid fibrils in Lewy bodies and Lewy neurites, the histopathological hallmarks of PD. Amyloid fibrillation is a progressive polymerization path starting from peptide/protein misfolding and proceeding through the transient growth of oligomeric intermediates widely considered as the most toxic species. Consequently, a promising approach of intervention against PD might be preventing α-synuclein build-up, misfolding and aggregation. A possible strategy involves the use of small molecules able to slow down the aggregation process or to alter oligomer conformation favouring the growth of non-pathogenic species. Here, we show that oleuropein aglycone (OleA), the main olive oil polyphenol, exhibits anti-amyloidogenic power in vitro by interacting with, and stabilizing, α-synuclein monomers thus hampering the growth of on-pathway oligomers and favouring the growth of stable and harmless aggregates with no tendency to evolve into other cytotoxic amyloids. We investigated the molecular basis of such interference by both biophysical techniques and limited proteolysis; aggregate morphology was monitored by electron microscopy. We also found that OleA reduces the cytotoxicity of α-synuclein aggregates by hindering their binding to cell membrane components and preventing the resulting oxidative damage to cells.

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Section: Discussionsupporting

confidence: 91%

Oleuropein aglycone stabilizes the monomeric α-synuclein and favours the growth of non-toxic aggregates

Palazzi

Bruzzone

Bisello

et al. 2018

Sci Rep

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“…Nevertheless, it should be also outlined that the ADME properties of this nutraceutical product, and consequently its biological effect in vivo, might be ascribed to the concurrent presence of all the PRES components. Finally, both oleuropein and hibiscus acid have shown interesting and promising neuroprotective effects also in in vivo models of neurodegenerative diseases [10,[40][41][42][43]. Moreover, oleuropein or its active metabolite, hydroxytyrosol, is found in a significant amount in the brain following oleuropein peripheral administration [44,45].…”

Section: Discussionmentioning

confidence: 99%

Olive Leaves and Hibiscus Flowers Extracts-Based Preparation Protect Brain from Oxidative Stress-Induced Injury

et al. 2020

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Oxidative stress (OS) arising from tissue redox imbalance, critically contributes to the development of neurodegenerative disorders. Thus, natural compounds, owing to their antioxidant properties, have promising therapeutic potential. Pres phytum (PRES) is a nutraceutical product composed of leaves- and flowers-extracts of Olea europaea L. and Hibiscus sabdariffa L., respectively, the composition of which has been characterized by HPLC coupled to a UV-Vis and QqQ-Ms detector. As PRES possess antioxidant, antiapoptotic and anti-inflammatory properties, the aim of this study was to assess its neuroprotective effects in human neuroblastoma SH-SY5Y cells and in rat brain slices subjected to OS. PRES (1–50 µg/mL) reverted the decrease in viability as well as the increase in sub-diploid-, DAPI-and annexin V-positive-cells, reduced ROS formation, recovered the mitochondrial potential and caspase-3 and 9 activity changes caused by OS. PRES (50–100 µg/mL) neuroprotective effects occurred also in rat brain slices subjected to H2O2 challenge. Finally, as the neuroprotective potential of PRES is strictly related to its penetration into the brain and a relatively good pharmacokinetic profile, an in-silico prediction of its components drug-like properties was carried out. The present results suggest the possibility of PRES as a nutraceutical, which could help in preventing neurodegenerative diseases.

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“…It was found that lipid oxidation level decreased significantly in the samples during the cold storage period [34]. In another study investigating the antioxidant activity of natural and synthetic polyphenol derivatives in O. europea, it was determined that the antioxidant activity of lipophilic acetyl derivatives was higher [35]. In a study using a mixture of extracts obtained from O. europea leaves and Hibiscus sabdariffa flowers, the mixture was reported to show a high degree of cytoprotective and antioxidant activity on human umbilical vein endothelial (HUVECs) cells [36].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Enzyme and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey

et al. 2020

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Phenolic compounds are secondary metabolites that are found ubiquitously in plants, fruits, and vegetables. Many studies have shown that regular consumption of these compounds could have a positive effect on our health. The aim of this study was to compare the phytochemical contents of the water extracts from three different plants used as folk remedies in Turkey: Aesculus hippocastanum, Olea europaea, and Hypericum perforatum. A liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS) analysis was performed to explore the phenolic profiles. The biological activities of these extracts were also evaluated in terms of their antioxidant activities (2,2-diphenyl-1-picrylhydrazyl DPPH, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid ABTS, Ferric Reducing Antioxidant Power Assay FRAP, cupric ion reducing antioxidant capacity CUPRAC, β-carotene, phosphomolybdenum, and metal chelating) and enzyme inhibitory properties (against acetylcholinesterase, butyrylcholinesterase, and tyrosinase). The aqueous extract of H. perforatum showed the highest levels of total phenolic, flavonoid, and saponin contents. Protocatechuic acid, vanillic acid, verbascoside, hesperidin, hyperoside, apigenin 7-hexosides, and quercetin were the most common compounds found in this species. The results confirm that A. hippocastanum, O. europaea, and H. perforatum represent a potential source of natural-derived molecules with positive properties that could be used as valid starting point for new food supplements, and drugs in the pharmaceutical, cosmetic, and food industries.

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Antioxidant activity of oleuropein and semisynthetic acetyl-derivatives determined by measuring malondialdehyde in rat brain

Cited by 32 publications

References 21 publications

Oleuropein aglycone stabilizes the monomeric α-synuclein and favours the growth of non-toxic aggregates

Oleuropein aglycone stabilizes the monomeric α-synuclein and favours the growth of non-toxic aggregates

Olive Leaves and Hibiscus Flowers Extracts-Based Preparation Protect Brain from Oxidative Stress-Induced Injury

Enzyme and Biological Activities of the Water Extracts from the Plants Aesculus hippocastanum, Olea europaea and Hypericum perforatum That Are Used as Folk Remedies in Turkey

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