Antioxidant activity of phenolic and flavonoid fractions of Cleome gynandra and Maerua angolensis of Burkina Faso

Abstract

Cleome gynandra L. and Maerua angolensis DC (Forsk) are two species of Capparidaceae family and of which the traditional uses are directed much against the inflammatory diseases, cancer and cellular ageing. The aim of this study is to measure the anti-FRAP, anti-ABTS and anti-DPPH activities through the n-hexane (n-H), dichloromethanolic (DCM), acetonitrile (ACN), ethyl acetate (EA), methanolic (MeOH) and n-butanol (n-BuOH) fractions of each species. We thus quantified the total phenolic and total flavonoids in different fractions. The whole of the tests were evaluated with a sample concentration at 100 µg/mL. Thus, three methods i.e., FRAP, ABTS and DPPH were used to estimate the total antioxidant capacity of the plants fractions. The total phenolic and total flavonoid were also determined spectrophotometrically using Folin-Ciocalteu and AlCl3 reagents, respectively. Butanolic fractions were give the bests anti-FRAP (535.961 µmol AAEAC/g of fraction), anti-ABTS (155.868 µmol TEAC/g of fraction) and anti-DPPH (81.109 µmol QEAC/ g of fraction) activities. The best phenolic content was obtained with n-butanol fraction (133.023 mg GAE/g of fraction, for C. gynandra), followed of methanolic fraction of M. angolensis (128.043 mg GAE/g of fraction). C. gynandra was give the best flavonoids contents with DCMF (50.09 %), followed of EAF (19.464 %). The obtained results allow justifying the traditional uses of these plants in the above-mentioned diseases.

Dietary Phytochemical Screening of Spider Plant (Gynandropsis gynandra (L.) Briq.) Accessions From Africa and Asia to Identify Genotypes for Use in Nutraceutical Breeding

“…In addition, phenolic compounds are reported to play essential roles in defense mechanisms against pathogens, parasites, and predators of plants, suggesting that the accessions that have a high content of phenolic compounds could be possible sources of genes for insect pests resistance. In this study, the mean of TPC of the spider plant accessions (123.3 mg GAE/g) was higher than the values reported by Jiménez-Aguilar and Grusak (2017), but lower than the findings of Meda et al (2013), who reported 133.0 mg GAE/g. In addition, other authors found lower levels of TPC (Somers et al, 2020), which were comparable to some African vegetables (Jiménez-Aguilar and Grusak, 2015).…”

“…These antioxidants neutralize/scavenge free oxygen and nitrogen radicals and prevent many diseases in the human body (Sarker and Oba, 2018e). Dietary phytochemicals of leafy vegetables, i.e., phenolics, are versatile compounds, such as phenol, coumarins, different groups of phenolic acids, and different groups of flavonoids, including flavonols, flavones, flavanols, flavanones, anthocyanins, chalcones, and different groups of non-flavonoids, including tannins, lignans and stilbenes that have been reported to have important antioxidant properties (Meda et al, 2013;Sarker and Oba, 2020e,f). The phytochemicals are distributed widely and occur in different concentrations within and across plant species.…”

Polyphenols contents and antioxidant potential of Nauclea latifolia Smith (Rubiaceae) acetonic fractions from Burkina Faso

“…The antioxidant power was carried out using the DPPH method according to the spectrophotometric method described by Meda et al, (2013). 20 In a test tube containing 0.375 ml of the sample (0.1 mg/ml) of each extract, 0.75 ml of the DPPH solution (20 mg /l) was introduced.…”

“…The antioxidant power was carried out using the DPPH method according to the spectrophotometric method described by Meda et al, (2013). 20 In a test tube containing 0.375 ml of the sample (0.1 mg/ml) of each extract, 0.75 ml of the DPPH solution (20 mg /l) was introduced. After 15 minutes' post-incubation of the mixture at room temperature in the dark, the absorbances were immediately read at 517 nm with a spectrophotometer (GENESYS 30).…”

Assessment of the stability and genotype-environment interaction of a spider plant (Cleome gynandra L.) collection in Burkina Faso: Application of the AMMI and GGE models

“…The nutritional composition of Cleome gynandra L. (Cleomaceae) makes this crop an added value to be taken into account in achieving food and nutritional security for consumers (Meda et al, 2013;Chand et al, *Corresponding author. E-mail: cheickboureimasakande@gmail.com.…”