Antioxidant activity of phenolic compounds from whole cottonseed by-product

Abstract

Cottonseeds are fed to high-producing dairy cows as a source of fat and highly-digestible fiber. Previously we reported the isolation and structure characterization of different phenolic compounds, quercetin, kaemferol and benzoic acid derivatives (1-9) from whole cottonseed by-product. In this paper the antioxidant activity of all isolated compounds was determined by measuring free radical scavenging effects using the Trolox equivalent antioxidant capacity assay and the coupled oxidation of β-carotene and linoleic acid (autoxidation assay). The range of phenolic compounds present in WCS (1.90 g/kg), assayed by the Folin-Ciocalteu method, and their antioxidant properties provides a valuable database for possible nutritional studies on the impact of diet on animal health and productivity.

Approach to Determination a Structure – Antioxidant Activity Relationship of Selected Common Terpenoids Evaluated by ABTS•+ Radical Cation Assay

“…Additionally, TEAC (Trolox equivalent antioxidant capacity) values were calculated for all the studied compounds. TEAC is defined as concentration of Trolox with the same antioxidant capacity as 1 mM solution of the compound under investigation [28]. In order to obtain a standard curve, antioxidant activity of Trolox was measured for the following concentrations [μM]: 5; 10; 15; 20 and 25.…”