Antioxidant activity of polysaccharide extracted from Ganoderma lucidum using response surface methodology

Kan, Yongjun; Ti-qiang, Chen; Wu, Yuangang; Wu, Jianguo; Wu, Jiaming

doi:10.1016/j.ijbiomac.2014.07.056

Cited by 113 publications

(57 citation statements)

References 37 publications

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“…Purification of GLP. GLP was extracted with a hot water extraction method [17]. The protein in the crude polysaccharide of Ganoderma lucidum was removed by following the sevag method [18].…”

Section: Extraction Andmentioning

confidence: 99%

Ganoderma lucidum Polysaccharide Enhanced the Antitumor Effects of 5-Fluorouracil against Gastric Cancer through Its Upregulation of NKG2D/MICA

Yang

Zhang

Yao

et al. 2019

International Journal of Polymer Science

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5-Fluorouracil (5-Fu) is one of the frequently used first-line cytotoxic drugs for chemotherapy against gastric cancer. Chemotherapy and immunotherapy are currently the main methods for treating gastric cancer. Immunotherapy can enhance the antitumor effect of chemotherapy drugs at the same time reducing its toxicity. The combination of these two therapies to treat cancer has become a mainstay and has received increasing attention in clinical practice. Ganoderma lucidum polysaccharide (GLP) is isolated from the Ganoderma lucidum fruiting body. Studies have shown that GLP has antitumor effects, where GLP does not directly kill tumors, rather exerting its antitumor function by stimulating immune cells including natural killer (NK) cells and T cells. In this study, the antitumor effect of GLP combined with 5-Fu was studied in vivo. At the same time, the associated mechanism of GLP combined with 5-Fu in gastric cancer cell lines BGC823 and SGC7901 was investigated in vitro. The results showed that GLP could stimulate the killing effect of NK-92 cells on gastric cancer cell lines BGC823 and SGC7901 and synergistically enhance the toxic effects of NK-92 cells on gastric cancer cell lines BGC823 and SGC7901. Moreover, GLP could further promote the activity of NK-92 cells by activating the NK cell activating receptor NKG2D and its downstream DAP10/PI3K/ERK signaling pathway.

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Section: Extraction Andmentioning

confidence: 99%

Ganoderma lucidum Polysaccharide Enhanced the Antitumor Effects of 5-Fluorouracil against Gastric Cancer through Its Upregulation of NKG2D/MICA

Yang

Zhang

Yao

et al. 2019

International Journal of Polymer Science

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“…The residue was washed with methanol three times to remove the TFA, and then dissolved in 2 mL distilled water. The pre-column monosaccharide derivatives were prepared using PMP reagents according to a previous method [18]. Man, Gal acid, Glu, Gal, and Ara were used as monosaccharide standards, and dissolved in distilled water (2 mM).…”

Section: Monosaccharide Composition Analysismentioning

confidence: 99%

Extraction optimization and characterization of polysaccharide antioxidants from <i>Pinellia ternata</i> (Thunb) Breit rhizome

Mo¹,

Liu²,

Tao³

et al. 2017

Trop. J. Pharm Res

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“…The effect of ultrasound exposure time on the yield of PRM is shown in Figure 1a. Various times (5,10,20,30, 40 and 50 min) were examined, while other extraction variables were kept as follows: ratio of water to material, 50 mL/g; ultrasound extraction temperature, 50 °C. The yield of PRM increased rapidly for increasing ultrasound exposure times ranging from 5 to 20 min.…”

Section: Single Factor Experimental Analysismentioning

confidence: 99%

“…Response surface methodology (RSM), a validated collection of statistical and mathematical techniques, is commonly used to optimize and evaluate complex experimental process factors and their interactions [4]. Furthermore, RSM needs less experimental trials and labor than many other optimized approaches [5]. The Box-Behnken design (BBD), one of the most used RSM methods, plays a pivotal role in arranging and interpreting the optimal experiments.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-Assisted Extraction, Antioxidant and Anticancer Activities of the Polysaccharides from Rhynchosia minima Root

Jia

Zhang

et al. 2015

Molecules

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Box-Behnken design (BBD), one of the most common response surface methodology (RSM) methods, was used to optimize the experimental conditions for ultrasound-assisted extraction of polysaccharides from Rhynchosia minima root (PRM). The antioxidant abilities and anticancer activity of purified polysaccharide fractions were also measured. The results showed that optimal extraction parameters were as follows: ultrasound exposure time, 21 min; ratio of water to material, 46 mL/g; ultrasound extraction temperature, 63˝C. Under these conditions, the maximum yield of PRM was 16.95%˘0.07%. Furthermore, the main monosaccharides of purified fractions were Ara and Gal. PRM3 and PRM5 exhibited remarkable DPPH radical scavenging activities and reducing power in vitro. PRM3 showed strong inhibitory activities on the growth of MCF-7 cells in vitro. The above results indicate that polysaccharides from R. minima root have the potential to be developed as natural antioxidants and anticancer ingredients for the food and pharmaceutical industries.

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Antioxidant activity of polysaccharide extracted from Ganoderma lucidum using response surface methodology

Cited by 113 publications

References 37 publications

Ganoderma lucidum Polysaccharide Enhanced the Antitumor Effects of 5-Fluorouracil against Gastric Cancer through Its Upregulation of NKG2D/MICA

Ganoderma lucidum Polysaccharide Enhanced the Antitumor Effects of 5-Fluorouracil against Gastric Cancer through Its Upregulation of NKG2D/MICA

Extraction optimization and characterization of polysaccharide antioxidants from <i>Pinellia ternata</i> (Thunb) Breit rhizome

Ultrasound-Assisted Extraction, Antioxidant and Anticancer Activities of the Polysaccharides from Rhynchosia minima Root

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