Antioxidant activity of protocatechuates evaluated by DPPH, ORAC, and CAT methods

Grajeda‐Iglesias, Claudia; Salas, Erika; Barouh, Nathalie; Baréa, Bruno; Panya, Atikorn; Figueroa-Espinoza, María-Cruz

doi:10.1016/j.foodchem.2015.07.119

Cited by 98 publications

(76 citation statements)

References 36 publications

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“…A way to prove this assumption is testing antioxidant activity of molecules with adjusted lipophilicity through our VesiCAT assays. It has been shown extensively that increasing alkyl chain length covalently linked on a phenolic moiety can significantly change (mostly through a nonlinear fashion) the molecular distribution in the system of interest, whereas its native scavenging/reducing capacity would not significantly be altered . Here, phenolic antioxidant GA was modified by lipophilization through esterification with different fatty alcohols and their antioxidant efficacy was tested by VesiCAT assay in liposome suspensions.…”

Section: Resultssupporting

confidence: 82%

Evaluation of Antioxidant Activity and Interaction with Radical Species Using the Vesicle Conjugated Autoxidizable Triene (VesiCAT) Assay

Durand

Zhao

Ruesgas‐Ramón

et al. 2019

Euro J Lipid Sci & Tech

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Liposomes can be used as therapeutic vehicles and membrane models to mimic cells, therefore developing new efficient assays to assess membrane oxidation of liposomes is of great importance. Their bilayer structure serves as an interesting model to investigate the antioxidant potential of compounds, as well as drug–membrane interaction. In this context, a new and high‐throughput method called “Vesicle Conjugated Autoxidizable Triene (VesiCAT)” that is based on specific UV absorbance spectral properties of a phospholipid probe, is described. The membrane oxidation under constant free radical flux generated by azo initiators, either in membrane or in aqueous phase, offers the possibility of extracting different but complementary informations in addition to the antioxidants efficacy. Thus, the VesiCAT assay represents an excellent tool not only to evaluate antioxidant capacity of molecules or extracts, but also to gain knowledge on their membrane distribution and their interaction with hydrophilic or hydrophobic reactive oxygen species. Practical Applications: Several methods have been developed to measure antioxidant capacity of molecules or extracts. However, most of them neglect the complexity of real systems; especially how the coexisting phases may get involved in the antioxidant performance. Moreover, most of the assays use accelerated oxidation tests, and they do not consider the effect of free radicals location on the antioxidant response. The VesiCAT method is a fast and efficient testing assay, implemented with a microplate reader. In addition, the aforementioned testing defect are considered and evaluated, rendering a more reliable and accurate way to predict the antioxidant potential. Thus, the VesiCAT is worth to serve as new routine assay for large‐scale antioxidant analysis, for both academic and industrial purposes, in alternative to conventional ones. The VesiCAT assay may represent a routine test for antioxidant ranking, with additional information regarding the antioxidants distribution and the evaluation of their capacity to interact with oxidant species in different cell regions.

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Section: Resultssupporting

confidence: 82%

Evaluation of Antioxidant Activity and Interaction with Radical Species Using the Vesicle Conjugated Autoxidizable Triene (VesiCAT) Assay

Durand

Zhao

Ruesgas‐Ramón

et al. 2019

Euro J Lipid Sci & Tech

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“…Eight anthocyanidins, seven anthocyanins and two synthesized 4′-hydroxy flaviliums DDPH, ABTS, hydroxyl radical scavenging activity, FRAP [20] Dried fruits and juices from chokeberry FRAP, ABTS [251] Protucatechuates DPPH, ORAC, CAT [252] Six deoxyanthocyanidins and cyaniding-3-glucoside DPPH, FRAP [253] Plant foods CUPRAC, ABTS [254] Anthocyanins from different varieties blueberries Inhibiting activity on lipid peroxidation, hydroxyl radical scavenging, superoxide anion radical, DPPH [255] Plan extracts DPPH, ABTS, AAPH [198] Commercial beverages (wines, beers, soft drinks and waters) TRAP, TEAC, FRAP [256] Popular antioxidants-rich US foods ABTS, DPPH [257] Commercially available vegetable juices (23) FRAP, DPPH, ABTS [258] Anthocyanidins, anthocyanidin-3-glucosides and portisins DPPH, FRAP [259] Plants extracts of industrial interest (30) DDPH, ABTS, FRAP, FRAP, SOD, ORAC [260] Food products ORAC, TEAC [231] Selected small fruits ABTS, FRAP, DPPH [204] Pulps of frozen fruits ABTS, DPPH, DMPD [190] CAT (conjugate autoxidizable assay); SOD (superoxide dismutase assay). Table 5.…”

Section: Samples Methods Referencesmentioning

confidence: 99%

“…In order to gather comprehensible information about the total antioxidant capacity of a food [184], at least two of these tests, and preferably all, should be combined, if possible, taking into account both the arguments for and against, and its applicability. Table 5 shows selected articles [20,190,198,204,231,[251][252][253][254][255][256][257][258][259][260] in which more than one criterion has been applied to real samples with practical purposes. Advantages and limitations of the most common chemical methods of determination of the antioxidant capacity are compiled in Table 6 [160, 161, 167, 171-173, 176, 184, 231, 261-263].…”

Section: Flavonoids -From Biosynthesis To Human Healthmentioning

confidence: 99%

Antioxidant Capacity of Anthocyanin Pigments

Martín¹,

Kuskoski²,

Navas³

et al. 2017

Flavonoids - From Biosynthesis to Human Health

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Anthocyanins are a family of natural pigments classified into the group of flavonoids, considered to be responsible for the color and taste of many fruits and vegetables, i.e. berries. Anthocyanins are common components of the human diet. Besides their interest as colorant because of their coloring properties, the study of anthocyanin compounds stems from their wide applicability in the prevention and even in the treatment of various human diseases. However, various aspects of the pharmacological roles of anthocyanins remain in the dark, having still several obstacles to the development of robust diets or prescribing lines on consumption of anthocyanins. The chemical structure of anthocyanins determines in large measure its capacity and efficacy as an antioxidant agent. In this study, the following aspects are reviewed: the antioxidant effect of anthocyanin pigments; the oxidative stress, the bioavailability after intake and biological aspects of anthocyanins, the method for measuring the antioxidant activity of anthocyanins, the relationship between structure and activity; and the influence of the anthocyanins in the antioxidant activity of wines. Finally an overview of some potential uses in food industry is attempted mainly focusing in the anthocyanin encapsulation topic. Attention has been paid to the more recent publications in the field.

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“…available for evaluating antioxidant capacities based on different principles and mechanisms of action. Among these methods, DPPH, ABTS and ORAC assays are used widely, 17,18 in which the former two are based on electron transfer reaction and the latter follows the principle of hydrogen atom transfer.…”

Section: -9mentioning

confidence: 99%

Comparative evaluations on phenolic antioxidants of nine adulterants and anti-inflammation of four alternatives with their original herb Erycibe schmidtii

Xue

Fan

et al. 2017

RSC Adv.

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Erycibe schmidtii is widely used as folk medicine in China for treatments of various inflammations. Recently, with reduction of its wild sources, various adulterants have been misused as substitutes. To distinguish reliable alternatives from various adulterants, total phenolics and antioxidant activities of E. schmidtii and its nine adulterants, as well as three marker compounds of E. schmidtii defined by Chinese Pharmacopoeia were simultaneously quantified. And HPLC fingerprints of these ten herbs were established. Compared with E. schmidtii (S1), Porana sinensis (S2), Porana sinensis var. delavayi (S3), Celastrus hindsii (S4), and Morinda umbellata (S5) exhibited similar or higher total phenols, flavonoids and tannins along with similar or greater capacities scavenging DPPHc, ABTS + c and AAPHc, contained similar or higher total amounts of the three marker compounds, and possessed higher similarities in HPLC profiles. The other five adulterants (S6-S10, i.e., Illigera parviflora, Morinda parvifolia, Piper puberulum, Piper kadsura and Iodes seguini in sequence) were identified as absolute fakes, thus were excluded from alternatives of S1. Further anti-inflammatory experiments with LPS-induced RAW264.7 macrophages cells on NO release and transcriptions of inflammatory factors iNOS, COX-2, IL-6 and IL-1b showed that S2 and S3 possessed higher anti-inflammation activities than and similar mechanisms to those of S1. Taken together, S2 and S3 could be the best potentially alternatives of E. schmidtii among the nine adulterants. S4 and S5 might be also considered as alternatives for they contained similar fundamental compounds and equally impressive anti-inflammatory potential with E. schmidtii concerning iNOS and COX-2.

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Antioxidant activity of protocatechuates evaluated by DPPH, ORAC, and CAT methods

Cited by 98 publications

References 36 publications

Evaluation of Antioxidant Activity and Interaction with Radical Species Using the Vesicle Conjugated Autoxidizable Triene (VesiCAT) Assay

Evaluation of Antioxidant Activity and Interaction with Radical Species Using the Vesicle Conjugated Autoxidizable Triene (VesiCAT) Assay

Antioxidant Capacity of Anthocyanin Pigments

Comparative evaluations on phenolic antioxidants of nine adulterants and anti-inflammation of four alternatives with their original herb Erycibe schmidtii

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