Antioxidant activity of selected phenols estimated by ABTS and FRAP methods

Biskup, Izabela; Golonka, Iwona; Gamian, Andrzej; Sroka, Zbigniew

doi:10.5604/17322693.1066062

Cited by 101 publications

(77 citation statements)

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“…ABTS is oxidized by potassium persulfate to its cation radical ABTS + , which is intensely colored. Our results showed that all the tested samples demonstrated a scavenging potential of the ABTS with varying inhibitory potential and interestingly, the MEHI demonstrating the highest ABTS inhibitory power and this result confirmed that the presence of higher phenolic level in the extract justified its highest scavenging property on ABTS radical [56]. Eventually, a good relationship was also observed between phenolics and antioxidant activity which further confirms this view [57].…”

Section: Discussionsupporting

confidence: 77%

In Vitro Free Radical Scavenging Activity and in Vivo Anticataract Potential of Hemidesmus Indicus Roots in Streptozotocin Induced Diabetic Rats

Tirumani

2017

Asian J Pharm Clin Res

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Objective: Hemidesmus indicus R. Br. (HI) (Indian sarsaparilla) is traditionally used in Indian medicine for human therapy. The present study was aimed to evaluate the antioxidant activity of HI root extracts, and its effects on delaying (or) management of cataract progression in streptozoticin (STZ) induced diabetic rats. Methods:The plant roots were extracted in different solvents, estimated total phenolic content, total flavonoid content and conducted free radical scavenging potential through 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2 azinobis (3-ethyl benzothiazoline-6-sulphonic acid diammonium salt (ABTS) NO, OH and reducing power assays. Further, the protective effect was evaluated in STZ induced diabetic rats through administered of ethyl acetate extract of HI (200 and 400 mg/kg body weight) orally for 8 weeks to study its effect on oxidative stress and diabetic cataract progression. Results:In primary results demonstrated that the ethyl acetate extract has been showed considerable free radical scavenging activity and significantly gain body weight, gradual decreasing blood glucose levels and delay cataract progression has been observed in HI treated rats. Furthermore, we observed significantly decreased oxidative stress markers, and restoration of the antioxidant system in HI treated rats. Lens sections from experimental animal again evidenced to conform the ameliorative effect of HI on diabetic cataract. Conclusion:This obtained result's confirmed that the protective effect of HI is attributed to the antioxidant activities of plant roots and its active principles.

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Section: Discussionsupporting

confidence: 77%

In Vitro Free Radical Scavenging Activity and in Vivo Anticataract Potential of Hemidesmus Indicus Roots in Streptozotocin Induced Diabetic Rats

Tirumani

2017

Asian J Pharm Clin Res

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“…The extract EEH had a significantly ( p < 0.05) more powerful inhibitory potential while the EFH and EFE extracts had the lowest scavenging potentials at all the concentrations. This result suggests that our extracted phytochemicals could act as radical scavengers owing to their hydrogen and electron donating capacity and their ability to delocalize/stabilize the resulting phenoxyl radical within the structure [40]. …”

Section: Resultsmentioning

confidence: 99%

Phenolic Content of Hypodaphnis Zenkeri and Its Antioxidant Effects against Fenton Reactions’ Mediated Oxidative Injuries on Liver Homogenate

et al. 2014

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Under oxidative stress conditions, endogenous antioxidant defenses are unable to completely inactivate the free radicals generated by an excessive production of reactive oxygen species (ROS). This state causes serious cell damage leading to a variety of human diseases. Natural antioxidants can protect cells against oxidative stress. Hypaodaphnis zenkeri (H. zenkiri) is a plant consumed as a spice in the Cameroonian diet, and its bark has been used in traditional medicine for the treatment of several diseases. The present study aims at investigating the antioxidant activity, which includes free radical scavenging and protective properties of an extract from H. Zenkiri against oxidative damage on a liver homogenate. The free radical assays determined the scavenging activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (OH), nitrite oxide (NO) and 2,2-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radicals and the enzymes, whose protection was to be considered in the liver homogenate, including superoxide dismutase, catalase, and peroxidase. The antioxidative activities were studied using the ferric reducing antioxidant power (FRAP), reductive activity, and phosphomolybdenum antioxidant power (PAP) methods. In addition, the phenolic contents of the extracts were examined. The results showed that these extracts demonstrated significant scavenging properties and antioxidant activities, with the hydro-ethanolic extract of the bark of H. zenkeri (EEH) being the most potent. This extract had the highest total polyphenol (21.77 ± 0.05 mg caffeic acid (CAE)/g dried extract (DE)) and flavonoids (3.34 ± 0.13 mg quercetin (QE)/g dried extract) content. The same extract had significantly greater protective effects on enzyme activities compared to other extracts. The high performance liquied chromatography (HPLC) profile showed higher levels of caffeic acid, OH-tyrosol acid, and rutin in the leaves compared to the bark of H. zenkeri. In conclusion, the ethanolic and hydro-ethanolic extracts of the bark and leaves from H. zenkeri showed an antioxidant and protective potential against oxidative damage.

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“…•+ was observed for phenolics with three or more hydroxyl groups [Biskup et al, 2013]. The antioxidant activity depends on the number and position of the hydroxyl groups of the aromatic ring binding site and the type of a substituent [Ishige et al, 2001].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars

Szychowski

Rybczyńska‐Tkaczyk

Gaweł-Bęben

et al. 2018

Pol. J. Food Nutr. Sci.

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Garlic (Allium sativum L.) has a reputation as a therapeutic agent for many different diseases such as microbial infections, hypertension, hypercholesterolaemia, diabetes, atherosclerosis and cancer. Health benefi ts of garlic depend on its content of biologically-active compounds, which differs between cultivars and geographical regions. The aim of this study was to evaluate and compare the biological activity of aqueous extracts from nine garlic varieties from different countries

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Antioxidant activity of selected phenols estimated by ABTS and FRAP methods

Cited by 101 publications

References 0 publications

In Vitro Free Radical Scavenging Activity and in Vivo Anticataract Potential of Hemidesmus Indicus Roots in Streptozotocin Induced Diabetic Rats

In Vitro Free Radical Scavenging Activity and in Vivo Anticataract Potential of Hemidesmus Indicus Roots in Streptozotocin Induced Diabetic Rats

Phenolic Content of Hypodaphnis Zenkeri and Its Antioxidant Effects against Fenton Reactions’ Mediated Oxidative Injuries on Liver Homogenate

Characterization of Active Compounds of Different Garlic (Allium sativum L.) Cultivars

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