Antioxidant activity of Trifolium resupinatum L. exposed to different extracts from leaves, flowers and shoots of Prangos ferulacea

Abstract

Prangos ferulacea is a member of Apiaceae family, is a popular fodder for livestock production and an important species in rangeland restoration. The objectives of the research are the comparative phytotoxic activity of aqueous and hydroalcoholic extracts obtained from different organs (flower, shoot and leaf) of P. ferulacea on antioxidant response of Trifolium resupinatum was investigated in a laboratory bioassay. Antioxidant enzyme activities including catalase (CAT), peroxidase (POD), ascorbic peroxidase (APX), and superoxide dismutase (SOD), in line with content of malondialdehyde (MDA) and hydrogen peroxide were measured. Results indicated that hydroalcoholic extract of P. ferulaceae flower possess the highest total phenolic content as well as highest phytotoxic effect on T. resupinatum. The highest antioxidant enzyme activity belonged to hydroalcoholic treatment. The treated T. resupinatum seedlings experienced lipid peroxidation at high extract concentrations (12% of hydroalcoholic and 100% of aqueous extract) as evidenced by increased concentration of MDA. In response to this, the activities of SOD, CAT, POD and APX increased at lower extract concentrations but significantly dropped as concentrations increased. According to results of this study, rehabilitation of T. resupinatum sites through the use of P. ferulaceae will probably not be successful.

Phytotoxic potential of Senna occidentalis (L.) Link extracts on seed germination and oxidative stress of Ipê seedlings

“…In our study, the increase in MDA content indicated a high ROS concentration, which may have exceeded the scavenging threshold of antioxidant enzymes in T. roseoalba and T. chrysotricha , when compared to other Ipê species. These findings are in agreement with previous studies that reported inhibition in the activity of antioxidant enzymes under allelopathic stress (Sunmonu & Van Staden ; Bazdar & Sadeghi ; Coelho et al . ).…”