Antioxidant activity of Tween-20 and Tween-80 evaluated through different in-vitro tests

Pérez-Rosés, Renato; Risco, Ester; Vila, Roser; Peñalver, Pedro; Cañigueral, Salvador

doi:10.1111/jphp.12369

Cited by 35 publications

(23 citation statements)

References 27 publications

(52 reference statements)

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“…T20 are considered hydrophilic in nature, hence the result of low DPPH scavenging activity was believed to be attributed by the hydrogen donation from the hydroxyl group (OH) present in the T20. This result was in accordance with a study by Pérez‐Rosés, Risco, Vila, Peñalver, and Cañigueral () that the limited DPPH values showed in Tween 20 and Tween 80 were due to the capability of hydrogen donation group. The slightly higher DPPH scavenging activity of β‐CD than T20 was attributed to the presence of more hydroxyl groups in cyclodextrin that able to donate more hydrogen atoms to reduce free radicals in the assay.…”

Section: Resultssupporting

confidence: 93%

Stability of Bioactive Compounds and Antioxidant Activities of Kenaf Seed Oil‐in‐Water Nanoemulsions under Different Storage Temperatures

Cheong

Tan

Nyam

2018

Journal of Food Science

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Section: Resultssupporting

confidence: 93%

Stability of Bioactive Compounds and Antioxidant Activities of Kenaf Seed Oil‐in‐Water Nanoemulsions under Different Storage Temperatures

Cheong

Tan

Nyam

2018

Journal of Food Science

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“…In this case, both an increase in antioxidant activity and its inhibition has been observed. At the same time, in a study by Pérez-Rosés, it was confirmed that Tween 20 and Tween 80 did not show scavenging activity on DPPH radical and its presence in the medium does not distort the antioxidant activity analysis results [50]. In this regard, for a more accurate interpretation of the data on antioxidant activity obtained in this work, additional studies on the effect of Tween 80 not only on the yield of polyphenols, but also on its effect on the antioxidant properties of the recovered substances are required.…”

Section: Discussionmentioning

confidence: 77%

Response Surface Modeling and Optimization of Polyphenols Extraction from Apple Pomace Based on Nonionic Emulsifiers

Skrypnik

Novikova

2020

Agronomy

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The factors affecting the efficiency of micelle-mediated extraction of phenolic compounds from apple pomace was investigated. Higher extraction efficiency by using as a solvent an aqueous solution of Tween 80 in comparison to Triton X-100, Span 20, Tween 20, 70% ethanol, and water was shown. Four independent variables (Tween 80 concentration, time, solvent-to material ratio, and pH) to enhance the recovery of polyphenols from apple pomace was investigated. Applying response surface methodology, the second order polynomial regression equation showing dependence of the yield of polyphenols on the extraction parameters was derived. The adjusted regression coefficient (R2 = 98.73%) and the lack-of-fit test (p > 0.05) showed a good accuracy of the developed model. The difference between observed and predicted values was no more than 3%. The optimal extraction conditions were found to be Tween 80 concentration of 1.14%, time of 65 min, solvent-to-material ratio of 104 mL g−1, pH of 3.8. Under optimal conditions the predicted total phenolic compounds content was 7.75 mg g−1. The obtained apple pomace extracts were characterized in terms of their antioxidant activity. The proposed extraction technology by using Tween 80 aqueous solution as a solvent allows obtaining the extracts with high content of polyphenols which are suitable for the applications in food, cosmetic, and pharmaceutical products requiring a surfactant to stabilize them.

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“…Tween 20 shares some structural similarity to Lipid A of Gram-negative bacteria, especially the short saturated fatty acids and has been implicated to affect the immune response and reactive oxygen species (ROS) production [ 34 ]. Tween 20 contains lauric acid that might be released and affect Tol-like receptors and induce NF-κB activation [ 56 , 57 ]. We demonstrate here that Tween does not play a role in the induction of IL-8 expression but that this is related to the MOI.…”

Section: Discussionmentioning

confidence: 99%

Expression profile analysis reveals that Aspergillus fumigatus but not Aspergillus niger makes type II epithelial lung cells less immunological alert

et al. 2018

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BackgroundAspergillus fumigatus is the main causative agent of aspergillosis. Infections rarely occur in immunocompetent individuals, indicating efficient clearance of conidia by pulmonary defense mechanisms. Other aspergilli like Aspergillus niger also cause infections but to a much lesser extent. Our previous studies showed that A. fumigatus and A. niger have different behavior in the presence of type II alveolar A549 epithelial cells. A. fumigatus conidia are more efficiently internalized by these cells and germination is delayed when compared to A. niger. In addition, hyphae that have escaped the epithelial cells grow parallel to the epithelium, while A. niger grows away from this cell layer.ResultsHere it is shown that global gene expression of A. fumigatus and A. niger is markedly different upon contact with A549 cells. A total of 545 and 473 genes of A. fumigatus and A. niger, respectively, were differentially expressed when compared to growth in the absence of A549 cells. Notably, only 53 genes (approximately 10%) were shared in these gene sets. The different response was also illustrated by the fact that only 4 out of 75 GO terms were shared that were enriched in the differentially expressed gene sets. The orthologues of A. fumigatus genes involved in hypoxia regulation and heat shock were also up-regulated in A. niger, whereas thioredoxin reductase and allergen genes were found up-regulated in A. fumigatus but down-regulated in A. niger. Infection with A. fumigatus resulted in only 62 up and 47 down-regulated genes in A549. These numbers were 17 and 34 in the case of A. niger. GO terms related with immune response were down-regulated upon exposure to A. fumigatus but not in the case of A. niger. This indicates that A. fumigatus reprograms A549 to be less immunologically alert.ConclusionsOur dual transcriptomic analysis supports earlier observations of a marked difference in life style between A. fumigatus and A. niger when grown in the presence of type II epithelial cells. The results indicate important differences in gene expression, amongst others down regulation of immune response genes in lung epithelial cells by A. fumigatus but not by A niger.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4895-3) contains supplementary material, which is available to authorized users.

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Antioxidant activity of Tween-20 and Tween-80 evaluated through different in-vitro tests

Abstract: Our findings raise concerns with regard to the indiscriminate use of Tween-20 and Tween-80 in clinical and laboratory testing, since they could influence the results that are assigned to the tested substance.

Cited by 35 publications

References 27 publications

Stability of Bioactive Compounds and Antioxidant Activities of Kenaf Seed Oil‐in‐Water Nanoemulsions under Different Storage Temperatures

Stability of Bioactive Compounds and Antioxidant Activities of Kenaf Seed Oil‐in‐Water Nanoemulsions under Different Storage Temperatures

Response Surface Modeling and Optimization of Polyphenols Extraction from Apple Pomace Based on Nonionic Emulsifiers

Expression profile analysis reveals that Aspergillus fumigatus but not Aspergillus niger makes type II epithelial lung cells less immunological alert

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