Antioxidant activity of various extracts from an edible mushroom Pleurotus eous

Abstract

Pleurotus eous is a pink edible oyster mushroom cultivated in Coimbatore. The aim of present study was to evaluate antioxidant potential of ethyl acetate, methanol, and hot water extracts from P. eous using various in vitro ROS/RNS generated chemical and biological models. Results demonstrated that the graded-dose (2-50 mg/mL) of various extracts markedly scavenged the DPPH, ABTS, and hydroxyl radicals, and showed metal chelating ability, reducing capacity in Fe 3+ /ferricyanide complex, and ferric reducing antioxidant power assays. In biological models, different extracts were found to inhibit lipid peroxidation in brain and liver homogenate. Total antioxidant activity was high in methanolic extract. Hot water extract showed higher amount of total phenol and methanolic extract showed higher amount of flavonoid as compared to other extracts. Based on EC 50 values it can be concluded that the various extracts from P. eous were good in antioxidant properties with methanol and ethyl acetate extracts being more effective. The results of this study showed that various extracts could serve as natural antioxidants owing to their significant antioxidant activity.

Phenolic composition and antioxidant properties of Pleurotus ostreatus and Pleurotus eryngii enriched with selenium and zinc

“…The total flavonoid content was 2.11 ± 0.19 and 1.26 ± 0.17 mg/g of extract, respectively. It was documented that major components of mushroom extract are phenolics and Pleurotus species exhibited different contents of total phenolic ranging from ~2 to >30 mg/g of extract [27,37,49]. In comparison with our earlier study [28], the results obtained for non-enriched mushrooms were slightly higher.…”

“…It has been reported that mushrooms contain different compounds of phenolics. Some authors have stated that mushrooms contain mainly phenolics acids [7,50], while others reported a high amount of flavonoids in extracts [5,37,51]. The total flavonoid content in different species of Pleurotus was found at the level from 1.2 to 2.9 µg/g of extract [49], even to 7.79 mg/g of extract [37].…”

“…The reduction of the DPPH radical was measured by monitoring the decrease in absorbance at 517 nm. The DPPH radical scavenging activity was calculated according to the formula [37]:…”

Comparison of antioxidant properties in cap and stipe of <i>Lentinula edodes </i> and #8211;a medicinal mushroom

“…The amount of total condensed tannins in cap aqueous extract was higher (3.26 ± 0.07 mg catechin/g extract) while stipe methanolic extract showed the lowest amount (1.23 ± 0.01 mg catechin/g extract). Sudha et al (2012) also reported that total flavonoid content of various extracts of Pleurotus eous varied considerably from 6.38-7.79 mg CAE/g extract. Ascorbic acid in the mushroom sample extracts are shown in Fig.…”

“…The reducing powers of methanolic and aqueous extracts of cap were 0.34 ± 0.004 and 0.54 ± 0.005, respectively at 25 mg/mL concentration. The reducing powers of methanolic and aqueous extracts of Pleurotus eous were reported to be 0.587 and 0.673 at 2.0 mg/mL, respectively (Sudha et al, 2012), however, cap and stipe methanolic extracts of Hypsizygus ulmarius and Calocybe indica were 2.46, 2.53, 1.71 and 2.05, respectively (Babu and Rao, 2013).The high reducing power exhibited by the extracts might be indicative of the hydrogen donating ability of the active species present in the extracts.…”