Antioxidant activity profile of extract and fraction of kersen (Muntingia calabura L.) fruits prepared by different methods

Abstract

The public widely uses kersen (Muntingia calabura L) fruit for consumption and treatment because Kersen fruit has various vitamins, minerals, and secondary metabolite compounds that reduce free radicals. The purpose of this study was to see the effectiveness of kersen fruit as an antioxidant agent. In this study, the samples used were ethanol extract (EE), Aqueous ethanol fraction (EF), ethyl acetate fraction (EAF), and n-hexane (HF) fraction from kersen fruit (Muntingia calabura L). The samples were evaluated by observing the antioxidant activity profile using various methods, i.e., nitric oxide, β-carotene bleaching assay, hydroxyl radicals, and iron chelating. The results of this study showed that the IC50 values of EE (31.05 μg/mL), AEF (33.86 μg/mL), FEA (40.48 μg/ml) gave powerful antioxidant activity while HF showed weak results (459 μg/ml). In the nitric oxide method. The hydroxyl radical method with IC50 values of 32.06 μg/mL (EE), 38.73 μg/mL (EF), 22.18 μg/mL (EAF) gave very strong activity, and 53.37 μg/mL (HF) with strong activity. The β-carotene bleaching method gave powerful antioxidant activity results with IC50 values <50 μg/mL in each sample. The Iron Chelation method showed weak antioxidant activity (IC50 > 200 μg/mL) of each sample. Based on the results obtained, it can be concluded that the antioxidant activity of extracts and fractions in kersen fruit has a different activity profile according to the test method used.

The Effect of Extraction Method on Flavonoid Content and Antioxidant Activity of Red Betel and Green Betel Extracts

“…The selection of the antioxidant activity test method is representative of evaluating the antioxidant potential of the ethanol extract of red and green betel leaves using the Hydrogen Atoms Transport (HAT) and Single Electron Transport (SET) mechanisms, including DPPH and ABTS method and redox mechanism in FRAP method. 20,21 The antioxidant potential of a sample is evaluated by determining the IC50 value or the potential of an antioxidant agent in reducing free radicals by 50%. 20,21 This can be used as a reference in determining the potential antioxidant activity of the ethanol extract of red and green betel leaves.…”

“…20,21 The antioxidant potential of a sample is evaluated by determining the IC50 value or the potential of an antioxidant agent in reducing free radicals by 50%. 20,21 This can be used as a reference in determining the potential antioxidant activity of the ethanol extract of red and green betel leaves. The results (Figure 1) of the antioxidant activity screening in the DPPH (A) and ABTS (B) assays showed a linear correlation of antioxidant activity with increasing concentrations in red and green betel leaf extract samples.…”

Phenolic and Flavonoid Content of Black Mulberry (Morus nigra L.) Stem and Their Evaluation Antioxidant and Cytotoxic Profile

“…The ability of extracts and stem fractions of M. nigra to scavenge hydroxyl radicals can be determined according to the procedure from previous studies 20, 21 . About 3 mL reaction mixture contained 1 mL of 1.5 mM FeSO4, 0.7 mL of 6 mM hydrogen peroxide, 0.3 mL of 20 mM sodium salicylate, and the extract and fraction of M. nigra stems with various concentrations (100-1000 µg/mL).…”

“…21,37 .Based on the results obtained from testing the antioxidant activity of the extract and stem fraction of M. nigra, it was shown…”

AKTIVITAS ANTIOKSIDAN EKSTRAK ETIL ASETAT BERBAGAI BAGIAN TANAMAN KESAMBI (Schleichera oleosa L.) DENGAN METODE DPPH

“…27 Kandungan senyawa fenolik-flavonoid dari ekstrak kesambi dapat menghambat aktivitas dari radikal dengan mekanisme transfer elektron maupun hidrogen yang berasal dari senyawa pada ekstrak etil asetat kesambi. 28,29 Daya antioksidan sampel akar, batang dan daun memiliki aktivitas aktioksidan yang termasuk kategori sangat kuat namun nilai IC50 masih lebih besar jika dibandingkan dengan vitamin C dengan IC50 3,25 µg/mL (Tukey HSD p<0,05, n=3). Senyawa yang diduga sebagai antioksidan dari ekstrak berdasarkan hasil uji skrining fitokimia yaitu senyawa polifenol antara lain golongan flavonoid, fenolik dan tanin.…”