The
emulsifying performance of glycerophospholipids alone is inferior
to proteins, etc., while the sphingomyelin (SM) and cholesterol (Chol)
naturally existing in biological membranes could interact with glycerophospholipids
to influence the polar lipid arrangement. Inspired by the natural
membranes, the effect of SM and Chol on the physicochemical stability
of soy phospholipid (SPL)-stabilized emulsions during storage or under
environmental stresses was determined. The results indicated that
the addition of SM and/or Chol could improve the storage stability
of the emulsions and protective effect on lutein significantly (p < 0.05). Except for UV irradiation, the addition of
Chol significantly improved the stability of the emulsions against
acid, salt, and heat. The strong intermolecular hydrogen bonds and
condensed assembly formed by SM and Chol contributed to the best stability
of SPL + SM + Chol-stabilized emulsions. The results gave insight
into improving the emulsifying properties of glycerophospholipids
with SM and Chol.