Antioxidant, analgesic, anti-inflammatory and antipyretic effects of polyphenols from Passiflora subpeltata leaves – A promising species of Passiflora

Saravanan, S.; Arunachalam, Karuppusamy; Thangaraj, Parimelazhagan

doi:10.1016/j.indcrop.2014.01.038

Cited by 45 publications

(23 citation statements)

References 27 publications

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“…Phenolics and flavonoids have been reported to be the most important natural aromatic chemical compounds responsible for the antioxidant capacity of plant extracts [21,47,65]. Most studies evaluated biological and pharmacological activities of various species of Passiflora have been focused on antioxidant properties of extracts from fruits [45,46,48,49,51,52,65]. In our study, extracts from leaves of three species of Passiflora were investigated because the leaves may be a valuable source of biomass, which is not widely used for economic purposes, and during fruit collection from cultivated species, the leaves are being removed.…”

Section: Resultsmentioning

confidence: 99%

“…ABTS radical scavenging test is one of the most extensively used antioxidant assays in plant extracts [47,65]. In this method, ANOVA analysis revealed significant differences in the antioxidant activity of In the third method with use of the FRAP radical model, ANOVA analysis revealed significant differences in the antioxidant activity of various concentrations of the extracts from leaves of P. caerulea (F (4,10)=7749.99, p<0.001), of P. alata (F (4,10)=13306.33, p<0.001), of P. incarnata (F (4,10)=2278.21, p<0.001).…”

Section: Resultsmentioning

confidence: 99%

“…FRAP [µM Fe2+/l] after the extracts expressed as trolox equivalent PI (control for PC and PA); p<0.05 crude extract, and value EC 50 =14.61 µg/ml for ethyl acetate fraction. Other authors[65] showed that the methanolic extract of P. subpeltata leaves exerted activity in FRAP test (1841.11 mmol Fe 2+ /mg extract), ABTS (3357.60 µM trolox equivalent/g extract) and DPPH (IC 50 =66.96 µg/ml) cation radical scavenging activities. In this study observed higher FRAP, ABTS and DPPH cation radical scavenging activities of extracts cold be attributed to the high level of polyphenolic content in extract from 100 g of leaves of P. subpeltata.…”

mentioning

confidence: 95%

“…sublanceolata, P. nitida, P. tenuifila, P. coriacea, P. foetida [50], extracts of peel of P. edulis [51,52], of seeds of P. edulis [53], of bark (exocarpium and mesocarpium) of P. edulis [54], various preparations of P. incarnata [55][56][57], fruit juices from seven passion fruit (Passiflora spp.) cultivars: P. edulis cultivars Purple, Frederick, Yellow, Pink, P. edulis f. flavicarpa, P. maliformis and P. quadrangularis [58], aqueous extract from leaf of P. edulis [59,60], ethanolic and fractionated extract from leaf of P. mucronata [61], fractionated extracts from leaves and stems of P. quadrangularis and P. maliformis [62], of P. alata [63], hydroethanol leaf extract of P. nitida [64], acetone extract of P. subpeltata leaves [65]. However, it should be emphasized that results of these studies are different not only due to various solvents used in conventional extraction but also by different in vitro and in vivo methods used to estimate the antioxidant activity and by different species, part of plants, chemical composition and environmental factors influencing various horticultural crops of Passiflora in countries around the world.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

et al. 2019

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Summary Introduction:. It is well documented that many species from Passifloraceae family can provide edible and nutritious fruits while the leaves of cultivated plants are renewable and waste material. This biomass may be further used in various sectors, especially as a bioactive food additive and as source of innovative pharmaceuticals, cosmetics or feed additives. The biomaterials and green chemistry are new sectors bioeconomy according to the high-level horizontal strategies and bio-based industries in Europe. In recent years, attention has been paid to the biological activity and phytochemical profiles of extracts from different species of Passiflora. However, there is little comparative studies using the same procedures and techniques in the same laboratory conditions for study of plant material obtained from the similar greenhouse conditions. Objective: This study was focused on the examination of antioxidative activities of low concentrations of crude extracts from leaves of Passiflora incarnata L., Passiflora caerulea L., and Passiflora alata Curtis. Methods: The activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging and ferric reducing antioxidant power (FRAP) methods. Results of study were supported by estimation of chemical composition with secondary metabolites profiling in extracts which were carried out previously for the same extracts from three Passiflora species. One-way ANOVA analysis revealed significant differences in the antioxidant activity of various concentrations of the extracts using the DPPH and ABTS radical models, and FRAP method. Results: Measurement of antioxidant capacity (expressed as trolox equivalent, TE) showed that the most active was extract of P. caerulea > P. alata > P. incarnata. Phytochemical analysis for extracts of P. caerulea and P. incarnata showed greater similarities in metabolites content than P. alata. However, comparative statistical analysis of antioxidant activity showed that despite this phytochemical similarities, extract from P. alata leaves had higher activities than extract from leaves P. incarnata. Antioxidant effect of extract from P. alata can be explain by terpenoids presented in this extract. In this work, there have been discussed activities against Acanthamoeba castellanii strain, antibacterial and antifungal activities against selected clinical microorganisms (Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, and Candida albicans, Micro-sporum gypseum), and anti-leukemic activities tested in human acute lymphoblastic leukemia cell lines for this extracts, which have been described in previous authors’ publications. Conclusion: Our current and previous studies showed that the same crude extracts from leaves of P. alata, P. caerulea, P. incarnata exerted not only antioxidant potential in vitro but also few interesting properties such as antibacterial, antifungal, amoebostatic, amoebicidal activities, which indicate the possibility of using these extracts in both a healthy diet and natural cosmetics. Leaves of this species may become an interesting source of biomaterials which can exert health-promoting effects.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

et al. 2019

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“…All extracts from herb of CM showed higher activity in 301.50 ± 1.34 F(4,10) = 10575.0, p = 0.000 F2H 60.42 ± 0.98 104.30 ± 1.16 + 148.06 ± 0.71 + 187.30 ± 1.09 + 267.87 ± 0.59 + F(4,10) = 7260.94, p = 0.000 F3H 53.65 ± 0.71 + 130.50 ± 2.66 + 175.86 ± 1.10 + 247.19 ± 0.33 + 299.75 ± 1.34 F(4,10) = 4341.64, p = 0.000 F1S ABTS radical scavenging method is one of the most extensively used antioxidant assays in plant extracts. It is applicable to both hydrophilic and lipophilic compounds in plant extracts [29]. Statistical analysis (ANOVA) revealed significant differences in the antioxidant activity of various concentrations of the CM extracts using the ABTS radical model (F(4,10)=265.0; p=0.000 for F1S; F(4,10)=211.1, p=0.000 for F2S, F(4,10)=1054.8, p=0.000 for F3S and F(4,10)=1564.9, p=0.000 for F1H, F(4,10)=1810.3, p=0.000 for F2H, F(4,10)=3112.3, p=0.000 for F3H) (tab.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Comparison of antioxidant activities of fractionated extracts from seedlings and herb ofChelidonium majusL. using DPPH, ABTS and FRAP methods

et al. 2016

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Summary Introduction: Our study is a part of a trend of studies on the antioxidative properties of Chelidonium majus extracts or their fractions suggesting that antioxidant activities may depend on total flavonoid and/or alkaloid contents. Objective: This study focused on the examination of antioxidative activities of full water extract, non-protein fraction and protein fraction of the extract from aerial parts of mature plants and young seedlings. Methods: Total flavonoid and alkaloid contents were evaluated by spectrometric methods. Quantitative determination of chelidonine, coptisine, sanquinarine, berberine was made by HPLC-UV. The antioxidative activities were evaluated using (1) 2,2-diphenyl-1-picrylhydrazyl (DPPH), (2) 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging and (3) ferric reducing antioxidant power (FRAP) methods. Results: All concentrations of herb extracts exhibited higher antioxidant capacities than extract from seedlings. Two antioxidant tests (DPPH, FRAP) showed that full water extract from herb had the highest antioxidant activity, while its non-protein fraction and protein fraction showed lower antioxidant activity. It was found that the full water extract from herb contained the highest concentrations of flavonoids and alkaloids when compared with other samples. Conclusion: Our findings suggest that chelidonine and coptisine especially could be responsible for the observed changes in the extract antioxidant activity, because these alkaloids were determined in the highest concentration in full water extract from herb. It cannot be also excluded that the observed variables values between extracts and their fractions from herb or from seedlings may also be the result of interactions between flavonoids and other chemical compounds.

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Chemical Investigation and Antinociceptive Activity Evaluation of Marrubium Vulgare L. Aqueous Extract

Aitbaba,

Kabdy,

Baslam

et al. 2024

Chemistry & Biodiversity

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Marrubium vulgare L. has a long history of use in traditional herbal medicine for the treatment of respiratory tract infections, inflammatory conditions, and pain. This study aimed to investigate the chemical composition, acute toxicity, and antinociceptive effects of the aqueous extract from M. vulgare leaves (AEMV). Antioxidant activity was evaluated using DPPH and reducing power assays. The chemical composition of AEMV was determined through LC‐MS/MS, and the levels of total phenolics, flavonoids, and condensed tannins were quantified. Acute oral toxicity was assessed in male Swiss mice with a single oral dose of AEMV (1, 2, 5 g/kg). The analgesic impact was examined through writhing, hot plate, and formalin tests. Our findings not only confirmed the safety of the extract in animal models but also revealed significant antioxidant activity in AEMV. High‐performance liquid chromatography (HPLC) analysis identified important bioactive compounds, with marrubiin being a major component. Furthermore, AEMV demonstrated robust antinociceptive properties in all conducted tests, highlighting its potential as a valuable natural source of bioactive compounds suitable for a wide range of therapeutic applications.

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Antioxidant, analgesic, anti-inflammatory and antipyretic effects of polyphenols from Passiflora subpeltata leaves – A promising species of Passiflora

Cited by 45 publications

References 27 publications

Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

Comparison of antioxidant activities of fractionated extracts from seedlings and herb ofChelidonium majusL. using DPPH, ABTS and FRAP methods

Chemical Investigation and Antinociceptive Activity Evaluation of Marrubium Vulgare L. Aqueous Extract

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