Antioxidant and acetylcholinesterase activities of three species of the family Lamiaceae

Sadashiva, C. T.; Naidoo, Yougasphree; Naidoo, JR; Kalicharan, B; Naidoo, G.

doi:10.3329/bjb.v43i3.21606

Cited by 5 publications

(4 citation statements)

References 19 publications

(17 reference statements)

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“…is a relatively unknown and under-explored member of the mint family that grows in semi-arid regions of southern Africa. Its pharmacological activities have not been verified, although it has free radical-scavenging and acetylcholinesterase-inhibiting properties [ 22 ]. The endophytic composition of its organs, its drought tolerance capability, and the plant growth-promoting properties of the endophytes have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

Ogbe

Gupta

Stirk

et al. 2023

Plants

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Endophytes are primarily endosymbiotic bacteria and fungi that colonize the interior tissues of their host plant. They enhance the host plant’s growth and attenuate adverse effects of biological stress. Endophytic species of many indigenous plants are an untapped resource of plant growth-promoting microorganisms that can mitigate abiotic stress effects. Thus, this study aimed to isolate endophytes from the roots and leaves of the medicinal plant Endostemon obtusifolius to evaluate their in vitro growth-promoting capacities and drought tolerance and to characterize the most promising species. Twenty-six endophytes (fourteen bacteria and twelve fungi) were isolated and cultured from the roots and leaves of E. obtusifolius. All 26 endophytes produced flavonoids, and 14 strains produced phenolic compounds. Of the 11 strains that displayed good free radical scavenging capability (low IC50) in the 1-1-diphenyl-1-picryhydrazyl radical scavenging assay, only three strains could not survive the highest drought stress treatment (40% polyethylene glycol). These 11 strains were all positive for ammonia and siderophore production and only one strain failed to produce hydrogen cyanide and solubilize phosphate. Seven isolates showed aminocyclopropane-1-carboxylate deaminase activity and differentially synthesized indole-3-acetic acid. Using molecular tools, two promising symbiotic, drought stress tolerant, and plant growth-enhancing endophytic species (EORB-2 and EOLF-5) were identified as Paenibacillus polymyxa and Fusarium oxysporum. The results of this study demonstrate that P. polymyxa and F. oxysporum should be further investigated for their drought stress mitigation and plant growth enhancement effects as they have the potential to be developed for use in sustainable agricultural practices.

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Section: Introductionmentioning

confidence: 99%

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

Ogbe

Gupta

Stirk

et al. 2023

Plants

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“…16 The total phenolic contents of the examined plant extracts are presented in Table 1. In this experiment, the highest phenolic content was found P. vestitus (2.8/100 g), followed by P. mollis (2.5/100 g) and P. speciosus (2.3/100 g).…”

Section: Total Phenols and Total Flavonoidsmentioning

confidence: 99%

In vitro Anti-oxidant Activity in Methanolic Extracts of five Pogostemon Spciece

Kaliyappan¹,

Nallasamy²,

Thirumuruthy³

2017

FRA

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A multifaceted peer reviewed journal in the field of Free Radicals and Antioxidants www.antiox.org | www.phcog.net Original Article INTRODUCTIONPlants have provided with all in terms of shelter, clothing, food, flavours and fragrances and medicine properties. Plants have formed the basis of complicated traditional medicine systems that have been in continuation for thousands of years to provide new remedies. The Lamiaceae contains about 236 genera and 6,900 to 7,200 species, 1 in this Pogostemon is one of the important genera and a large widespread genus having 114 species in India. Both roots and leaves are mostly used by tribal people in this genus like the fresh root or poultice of the leaves is applied on the snake bites particularly Phursa (Echis carinatus) snake bite, uterine haemorrhage, leaf extract used as an insect repellent, treatment of food poison, vomiting and stomach troubles, respiratory tract infection and pollen and nectar used as a source of Panagol honey in Maharashtra. The superoxide anion (O 2 ) hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (OH) are the form of active oxygen and free radicals. These are constantly formed in human body through normal metabolic action. Hence, this leads to a variety of biochemical and physiological lesion, often consequent in metabolic destruction and cell death. 3 The synthetic antioxidants were creating the genotoxic effect 4 and other chronic disease.5 Therefore, the antioxidant properties of secondary metabolites have formed the foundation of many applications in a preservation process as pharmaceuticals, alternative medicine and natural therapies. Here the studies to explore the five Pogostemon species which have been scientific conform for their potential medicinal values. MATERIALS AND METHODS Preparation of plant extractsPogostemon vestitus, P. mollis, P. speciosus, P. wightii and P. nilagiricus were colocted belongs to the family Lamiaceae were collected from natural forest of Western Ghats of Coimbatore, Tamil Nadu. The specimen was identified by Dr. M. Murugesan, Scientist B, Botanical Survey of India, Shillong. Fresh plant materials were washed under running tap water, air-dried and powdered. About 50 g of coarsely powdered plant materials (50 g/250 ml) were extracted in a soxhlet extractor for 8-10 hrs, with methanol. The extract obtained was then concentrated using a vacuum evaporator and weighted. For stock solutions, 1 mg/ml of methanolic extract was dissolved in dimethyl sulfoxide (DMSO). Determination of Total phenolsTotal phenolics were quantified and expressed as Gallic acid equivalents according to a method proposed by Singleton et al. 7 About 3.9 ml of distilled water and 0.5 mL of Folin-ciocalteau reagent were added to 0.1ml of the extract in a tube and incubated at room temperature for 3 min after which 2 mL of 20% sodium carbonate was added and kept in a boiling water bath for 1 min. Phenols react with phosphomolybdic acid in the Folin-ciocalteau reagent in alkaline medium and produce a blue coloured complex (molybdenum blue) th...

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“…Accordingly, Uddin et al [38] and Boonsong et al [47] reported the fact that polyphenols attributed to both antioxidant and AChE inhibitory activity significantly. In fact, polyphenolic compounds such as gallotannins, condensed tannins and flavonoids contribute directly to the antioxidant activity and AChE inhibitory activity of plant extracts [48]. The total polyphenol and total flavonoid contents were moderately correlated with the AChE inhibitory activity however, the degree of correlation was lower than that in the correlation observed with the total antioxidant activity by four assays.…”

Section: Resultsmentioning

confidence: 86%

“…On the other hand, the extracts containing non-polar phytochemicals such as alkaloids might be responsible for high inhibitory activity particularly on AChE. In fact, the alkaloids are the main secondary metabolites which are of concern in the synthesis of novel anticholinesterase drugs rather than polyphenolic/flavonoid compounds [48,51,52].…”

Section: Resultsmentioning

confidence: 99%

Acetylcholinesterase Inhibitory Activity and Antioxidant Activity of Selected Medicinal Plant Extracts Used Against Cognitive Dysfunction and Memory Loss in Sri Lankan Traditional Medicine

Attanayake

Jayatilaka²

2018

Asian J Pharm Res Dev

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The objectives of the study were to determine the acetylcholinesterase (AChE) inhibitory activity and antioxidant activity in the selected Sri Lankan medicinal plants. AChE inhibitory activity of the selected medicinal plant extracts was determined using the Ellman’s method. The antioxidant activities were determined by four in vitro methods namely DPPH assay, FRAP assay, NO assay and TBA acid method. The contents of total polyphenols and flavonoids were determined quantitatively.Out of the ten selected medicinal plant extracts Abrus precatorius, Centella asiatica, Strychnos nux-vomica and Ricinus communis showed IC50 values < 200 µg/mL for AChE inhibitory activity. A high antioxidant activity was shown in the extracts of Cardiosprmum halicacabum, Centella asiatica and Ricinus communis in the four selected antioxidant assays. The total polyphenol content and total flavonoid content was in the range of 0.55-7.30 mg/g DW and 19.08±0.29-1283.08± 0.09 µg/gDW. The extracts of A. precatorius, C. asiatica, S. nux-vomica and R. communis are deserved to be as potent sources of AChE inhibitors as well as natural antioxidants. Considering the complex multifactorial etiology of Alzheimer's diseases, these plant extracts would be apt candidates for the development of novel pharmaceutical agents in the management of Alzheimer's diseases.

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Antioxidant and acetylcholinesterase activities of three species of the family Lamiaceae

Cited by 5 publications

References 19 publications

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

In vitro Anti-oxidant Activity in Methanolic Extracts of five Pogostemon Spciece

Acetylcholinesterase Inhibitory Activity and Antioxidant Activity of Selected Medicinal Plant Extracts Used Against Cognitive Dysfunction and Memory Loss in Sri Lankan Traditional Medicine

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