Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus and Zizyphus Vulgaris Methanol Extracts

Abstract

Objective: This report is an attempt to study the phenolic composition of Rubus fruticosus (RFE) and Zizyphus vulgaris (ZVE) methanol extracts and evaluate their antioxidant and anti-inflammatory effects in-vitro and in-vivo. Methods:Total phenolic and total flavonoids contents of extracts were determined by spectrophotometric methods. Phenolic compounds were identified by HPLC-TOF/MS. The antioxidant activities were evaluated in vitro using DPPH, ABTS and FRAP assays. The effect of RFE and ZVE on DNA cleavage induced by H2O2 Results:The phytochemical analysis of these extracts showed that RFE possesses higher polyphenolic and flavonoid content than ZVE. in the same way RFE exerted the highest antioxidant capacity with IC UV-photolysis was also investigated. The antioxidant effect of RFE and ZVE was tested in vivo using the blood total antioxidant capacity test in mice. On the other hand, the anti-inflammatory activity was assessed in vivo using two models of acute inflammation ear edema and vascular permeability. 50 value of 14 µg/ml in DPPH assay, 1.58 mmol of Trolox E/mg extract and 3.39 of mmol FesO4/mg extract in ABTS, and FRAP assay respectively. The studied extracts showed a concentration-dependent protective effect on DNA cleavage induced by H2O2 UV-photolysis. The daily oral administration of 200 mg/kg of RFE or ZVE during three weeks showed an improvement of the blood total antioxidant capacity; the HT50 Conclusion:The results obtained in this investigation showed that RFE possesses strong antioxidant and anti-inflammatory potential in comparison with ZVE, which may be attributed to the presence of polyphenolic phytoconstituents. values were151.45 min and 146.72 min for the groups treated with RFE and ZVE, respectively versus 122.5 min for the control group. The topical application of 2 mg/ear of RFE inhibited the croton oil-induced ear edema by 75.72%, while the inhibition exerted by ZVE was 64.24%. These inhibitions were higher than that of indomethacin, used as a reference. Moreover, the oral administration of 400 mg/kg of RFE inhibited significantly (33.57%) acetic acid induced vascular permeability in mice. However, this effect was lower than this of indomethacin. The inhibition effect exerted by ZVE was not significant.

Antioxidant and Anti-Inflammatory Properties of a Series of New7,8-Disubstituted Theophylline Containing a Pyrazole Ring

“…Antiradical activity of phenylalyl derivative 13 remains at 90%. In general, the following should be noted: The highest antioxidant effect was observed for 1-(1,3-dimethyl-2,6-dioxo-7-arylalkyl-(alkenyl-)-2,3,6,7-tetrahydro-1H-purine-8-yl)-5-(4-methoxyphenyl)-1H-pyrazole-3-carboxylates (11)(12)(13), which is consistent with the literature data [13].…”

“…When DPPH interacts with substances that capable of binding to free radicals, produce products that are mostly colored in shades of yellow and do not absorb light at the above wavelength. This method is fast, easy to implement and allows for the screening of large arrays of investigated substances [10,11].…”

In Vitro Antioxidant Activity of Bark Extracts of Oroxylum Indicum (L) Vent

“…While working with Turkish Tea polyphenols and alkaloid Erol et al [30] proposed that alkaloid content is linked with antioxidant activity. Rackova et al [31][32][33] suggested that probably high lipophilicity of the alkaloids is responsible for antiradical reactivity.…”

Antioxidant Potential Profile of Pajanelia Longifolia (Willd.) K. Schuman.; Potential New Sources of Natural Antioxidant.

“…Maximum radical scavenging activity was exhibited by the acetone extract of P. longifolia implying that phenol and flavonoid content is positively correlated to the antioxidant activity of a plant [16][17][18]. Considering evidence from literature [19][20][21][22], the involvement of phenols and flavonoid in antioxidant activity can be established. Thus, the present findings in this context, summarizes with the same concept without any deviation.…”