Antioxidant and anti-inflammatory activity of fruit, leaf and branch extracts of Paliurus spina-christi P. Mill

Abstract

ABSTRACT:In this study, it is aimed to investigate antioxidant and anti-inflammatory activities of ethanol extracts and their n-hexane, chloroform, ethyl acetate, and aqueous ethanol fractions of the fruits, leaves, and branches of Paliurus spinachristi P. Mill. (Rhamnaceae). The antioxidant activity of the extracts was tested by DPPH and ABTS methods and the results were expressed as IC50 values. The total phenolic content was determined by Folin-Ciocalteu method and the results were expressed as mg of gallic acid equivalent per g dry extract. Anti-inflammatory activity of extracts were evaluated against 5-lipoxygenase enzyme. In particular, all extracts obtained from branches of P. spina-christi, except hexane extract, showed strong antioxidant activity against DPPH and ABTS radicals. Ethyl acetate and ethanol extracts of branches of P. spina-christi showed the highest antioxidant activity in DPPH and ABTS assays with IC50 values of 15.54 and 22.06 µg/ml, respectively. Also, the highest total phenol content was found in the ethyl acetate extract of branches of P. spina-christi (286.6 mg/g). Ethyl acetate and ethanol extracts of leaves of P. spina-christi with IC50 values of 66.28 and 76.75 µg/ml were found to have the best anti-inflammatory activity in the lipoxygenase (LOX) inhibition assay used to evaluate anti-inflammatory activities of extracts. These results demonstrate that ethanol and ethyl acetate extracts from branches and leaves of Paliurus spina-christi have significant antioxidant and anti-inflammatory activity. Also, these results confirm the folk use of the plant as an antiinflammatory medicine.

Anticancer, Antioxidant and Anti-Inflammatory Activities of Chloroform Extracts from Some Centaurea Species

“…Plant samples were collected in the flowering periods from the different regions of Istanbul in 2009 and were identified by Dr. Gizem BULUT and Dr. ŞükranKültür ( Table 1). The extraction was done according to the method proposed by Ali et al with minor modifications [4]. For the first extraction process of plants, methanol was used.…”

“…The HepG2 viability and IC50 were evaluated by Vybrant ® MTT Cell Proliferation Assay Kit. Free radical scavenging capacity of extracts were evaluated according to the previously reported procedure using the stable DPPH [4]. ABTS radical-scavenging activity of extracts was determined according to the previously reported procedure [4].…”

“…Free radical scavenging capacity of extracts were evaluated according to the previously reported procedure using the stable DPPH [4]. ABTS radical-scavenging activity of extracts was determined according to the previously reported procedure [4]. The anti-inflammatory activity was tested according to the method described by Phosrithong et al [4].…”

“…ABTS radical-scavenging activity of extracts was determined according to the previously reported procedure [4]. The anti-inflammatory activity was tested according to the method described by Phosrithong et al [4]. Total phenolic contents of extracts were measured using Folin-Ciocalteau reagent [4].…”

KARAÇALI (Paliurus spina-christi Mill.) BALININ KARAKTERİSTİK ÖZELLİKLERİ

“…Karaçalı bitkisinin çiçekleri Mayıs-Temmuz ayları arasında hava şartlarına bağlı olarak gelişmekte olup, sarı renkli, orta tatlılıkta ve hafif acılığa sahip olan ve çok hızlı kristalleşen ballar vermektedir. Karaçalı halk arasında mesih dikeni, draga dikeni, öküz gözü, ilme, çaltı dikeni, sarı çalı, sarı diken, gibi yerel isimlerle de adlandırılmakta olup, idrar söktürücü, antiromatizmal, hipokolesterolemik, tonik ve kronik obstrüktif akciğer hastalığı tedavisinde geleneksel olarak kullanılmaktadır (Zor vd., 2017;Şen., 2018). Karaçalı meyve özütlerinin antidiyabetik etkisi olduğu da belirtilmektedir (Takim,2018).…”