Antioxidant and anti-inflammatory functionality of ten Sri Lankan seaweed extracts obtained by carbohydrase assisted extraction

Fernando, Ilekuttige Priyan Shanura; Sanjeewa, K. K. Asanka; Samarakoon, Kalpa W.; Lee, Won Woo; Kim, Hyunsoo; Ranasinghe, P.; Gunasekara, U. K. D. S. S.; Jeon, You‐Jin

doi:10.1007/s10068-018-0406-1

Cited by 37 publications

(25 citation statements)

References 31 publications

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“…Our results also indicated a strong correlation (at P ≤ 0.05) between the antioxidant activity using DPPH, reducing power and TAC with the estimated total phenolic content of seaweeds which were in adherence to studies by Ismail et al (2016) and Ismail (2017). In addition, some studies reported the antioxidant activities of the seaweeds are associated with the phenolic algal content (Shanura Fernando et al, 2018). Nowadays, there is a growing concern on the discovery of natural antioxidants mainly because biologically active phytochemicals are largely more secure than manufactured synthetic analogies and for their conservative role in the improvement of disease like cancer, atherosclerosis, arthritis, diabetes, alzheimer's and aging (Santos-Sánchez et al, 2019).…”

Section: Total Phenolic Content (Tpc)supporting

confidence: 89%

“…Also, the acetone extract of Sargassum wightii and the benzene extract of Sargassum polycystum exhibited DPPH antioxidant activity with 43% and 22%, respectively (Unnikrishnan et al, 2015). In a recent study, the Celluclast (a preparation used to degrade cellulose into glucose) enzymatic extract of Sargassum polycystum revealed a strong DPPH scavenging activity estimated to be 57.23% (Shanura Fernando et al, 2018).…”

Section: Antioxidant Assaysmentioning

confidence: 99%

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In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes

et al. 2020

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In this study crude extracts of Turbinaria decurrens, Padina pavonica, Sargassum muticum and Sargassum acinarium (Phaeophyta); Ulva lactuca (Chlorophyta) and Pterocladia capillacea (Rhodophyta) seaweeds were tested to evaluate their antioxidant properties and antidiabetic potential on α-amylase and α-glucosidase starch hydrolyzing enzymes. The results showed that all analyzed seaweeds exhibited antioxidant effects using DPPH (2,2-Diphenyl-1-picrylhydrazyl), reducing power and total antioxidant capacity assays in addition to antidiabetic activity that all depended on the species and the extract solvent. Among the tested extracts, acetone extract of Turbinaria decurrens showed the highest antioxidant activity and inhibitory effects for α-amylase (96.1%) and α-glucosidase (97.4%), respectively which was related with its total phenolic content and antioxidant activity. In vitro, the extract showed no toxicity against fibroblast normal cell lines at lower concentration of 250 µg/ml. Gas Chromatography-Mass Spectrum analysis (GC-MS) of this acetone extract showed the presence of different bioactive compounds mainly cyclotrisiloxane, hexamethyl which could be responsible for its antioxidant and antidiabetic activities. The study results suggest that brown seaweeds especially T. decurrens can be used as antioxidant ingredients and as potent reducing drug for postprandial hyperglycemia.

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Section: Total Phenolic Content (Tpc)supporting

confidence: 89%

Section: Antioxidant Assaysmentioning

confidence: 99%

In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes

et al. 2020

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“…With respect to the prevention and/or treatment of chronic inflammation, dietary compounds are receiving attention due to their safety and lack of adverse effects [26]. Among various dietary approaches, treatment with the edible oil RBO was investigated for its anti-inflammatory effects in the current study.…”

Section: Discussionmentioning

confidence: 99%

Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages

Lee

Park

et al. 2019

PLoS ONE

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Previous studies have revealed the anti-inflammatory properties of rice bran oil (RBO), but the detailed mechanisms are poorly understood. Recent studies on the molecular/cellular anti-inflammatory mechanisms of dietary components have demonstrated that mitochondrial respiration plays a key role in macrophage functioning. Since dietary lipids are major substrates for mitochondrial respiration through β-oxidation, the current study examined whether RBO regulates inflammatory responses by modulating mitochondrial energy metabolism. Palm oil (PO), enriched with palmitic acid which are known to be effectively taken up by cells and used for oxidative phosphorylation, served as a positive control. In the in vitro model of LPS-stimulated RAW 264.7 murine cells, the levels of pro-inflammatory cytokines (IL-6 and TNF-α) in the culture supernatant were significantly reduced by RBO treatment. In contrast, secretion of the anti-inflammatory cytokine IL-10 was upregulated by RBO. Transcription of genes encoding inflammatory mediator molecules (COX-2 and iNOS) and expression of activation markers (CD80, CD86, and MHC-II) in LPS-stimulated RAW 264.7 cells were suppressed by RBO. Mitochondrial respiration (as assessed by an extracellular flux analyzer) increased upon RBO treatment, as the basal respiration, maximal respiration, ATP production, and spare respiratory capacity were upregulated. In an in vivo study, C57BL/6 mice were fed a negative control diet containing corn oil (CO), PO, or RBO for 4 weeks, and bone marrow-derived macrophages (BMDM) were isolated from their tibias and femurs. In pro-inflammatory M1-polarized BMDM (M1-BMDM), the RBO-induced suppression of IL-6 and TNF-α was recapitulated in vivo. Mitochondrial respiration in M1-BMDM also increased following the RBO intervention and the PO control treatment as compared to CO fed negative control. Overall, the current study for the first time demonstrates that RBO regulates inflammatory responses in murine macrophages by upregulating mitochondrial respiration. Further clinical studies are required to validate the animal study.

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“…Following up on the preliminary screening [25], Sargassum polycystum samples for the mass extraction were collected from the south-west coast of Sri Lanka. After washing with tap water, the samples were lyophilized and ground into a powder.…”

Section: Collection and Extraction Of Fucoidan Rich Fractionmentioning

confidence: 99%

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

et al. 2020

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Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 µg mL−1 and 93.62 ± 3.53 µg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals.

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Antioxidant and anti-inflammatory functionality of ten Sri Lankan seaweed extracts obtained by carbohydrase assisted extraction

Cited by 37 publications

References 31 publications

In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes

In vitro potential activity of some seaweeds as antioxidants and inhibitors of diabetic enzymes

Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages

Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells

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