Antioxidant and anti-inflammatory potential of spirulina and thymoquinone mitigate the methotrexate-induced neurotoxicity

Behairy, Alaa; Elkomy, Ashraf; Elsayed, Faten; Gaballa, Mohamed M. S.; Soliman, Ahmed; Aboubakr, Mohamed

doi:10.1007/s00210-023-02739-4

Cited by 11 publications

(3 citation statements)

References 66 publications

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“…Oxidative stress plays a significant role in the process of tissue damage. It occurs when an imbalance between the production of reactive oxygen species (ROS) and the body’s ability to neutralize or repair their harmful effects arises ( 44 – 47 ). The overproduction of oxidative stress leads to several brain diseases ( 48 , 49 ).…”

Section: Discussionmentioning

confidence: 99%

The potential neuroprotective of luteolin against acetamiprid-induced neurotoxicity in the rat cerebral cortex

Albrakati

2024

Front. Vet. Sci.

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Acetamiprid is a class of neuroactive insecticides widely used to control insect pests. The current study aimed to investigate the potential neuroprotective effects of luteolin against acetamiprid-induced neurotoxicity in the rat cerebral cortex. Four equal groups of adult male rats (10 in each): control, acetamiprid (40 mg/kg for 28 days), luteolin (50 mg/kg for 28 days), and acetamiprid+luteolin cotreatment were used. Acetamiprid was shown to alter the oxidative state by increasing oxidant levels [nitric oxide (NO) and malondialdehyde (MDA)] and decreasing antioxidants [glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), and catalase-(CAT)], with increased activity of nuclear factor erythroid 2–related factor 2-(Nrf2). Likewise, acetamiprid increases the inflammatory response, as evidenced by increased interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and nuclear factor kappa B-(NF-κB). In contrast, the treatment with luteolin brought these markers back to levels close to normal, showing that it protects neurocytes from oxidative damage and the neuroinflammation effects of acetamiprid-induced inflammation. Luteolin also demonstrated a neuroprotective role via the modulation of acetylcholinesterase (AChE) activity in the cerebral cortex tissue. Histopathology showed severe neurodegenerative changes, and apoptotic cells were seen in the acetamiprid-induced cerebral cortex layer, which was evident by increased protein expression levels of Bax and caspase-3 and decreased Bcl-2 levels. Histochemistry confirmed the neuronal degeneration, as proven by the change in neurocyte colour from brown to black when stained with a silver stain. Luteolin may have a neuroprotective effect against biochemical and histopathological changes induced by acetamiprid in the rat cerebral cortex.

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Section: Discussionmentioning

confidence: 99%

The potential neuroprotective of luteolin against acetamiprid-induced neurotoxicity in the rat cerebral cortex

Albrakati

2024

Front. Vet. Sci.

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“…Brain tissue is particularly vulnerable to oxidative damage due to its low antioxidant capacity, high energy demand, and the presence of polyunsaturated fatty acids. [ 1,2 ] The chemotherapeutic cyclophosphamide (CP), frequently utilized as an alkylating agent for its anticancer and immunosuppressive properties, can initiate neuroinflammation and oxidative damage in neuronal cells. [ 3 ] Two metabolites of CP are acrolein and phosphoramide mustard.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the effects of curcumin and piperine on cyclophosphamide‐induced brain injury in rats

Aygörmez,

Maraşli

2024

J Biochem & Molecular Tox

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Cyclophosphamide (CP) is an antineoplastic drug widely used in chemotherapy. Curcumin (CUR) and piperine (PP) show a protective effect on neurodegenerative and neurological diseases. This research was designed to measure several biochemical parameters in the brain tissue of CP‐applied rats to investigate the impact of combined CUR–PP administration. The study evaluated six groups of eight rats: Group 1 was the control; Groups 2 and 3 were administered 200 or 300 mg/kg CUR‐PP via oral gavage; Group 4 received only 200 mg/kg CP on day 1; Groups 5 and 6 received CP + CUR‐PP for 7 days. Data from all parameters indicated that CP caused brain damage. Phosphorylated TAU (pTAU), amyloid‐beta peptide 1–42 (Aβ1–42), glutamate (GLU), and gamma amino butyric acid (GABA) parameters were the same in Groups 4, 5, and 6. On the other hand, 8‐hydroxy‐2‐deoxyguanosine (8‐OHdG), nitric oxide (NO), interleukin‐6 (IL‐6), nuclear factor kappa beta (NF‐kβ), malondialdehyde (MDA), and tumor necrosis factor‐alpha (TNF‐α) levels in the CP + CUR‐PP groups were lower than those in the CP group (p < 0.05). However, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and reduced glutathione (GSH) parameters were higher in the CP + CUR‐PP groups compared to the CP group (p < 0.05). It is thought that the similarity of Groups 5 and 6 with Group 4 in Aβ1–42, pTAU, GLU, and GABA parameters hinder the determination of treatment protection however, they might have a therapeutic effect if the applied dose or study duration were changed. This study attempted to evaluate the effects of a CUR–PP combination on CP‐induced brain damage in rats by measuring biochemical parameters and performing histopathological examinations. Based on the findings, this CUR–PP combination could be considered an alternative medicine option in cases with conditions similar to those evaluated in this study.

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“…It has been extensively studied for its wide array of therapeutic and pharmacological activities, including but not restricted to its anti-oxidant, anti-inflammatory and anti-apoptotic effects. 24–26 …”

Section: Introductionmentioning

confidence: 99%

The Protecting Role of Black Seed Oil and Its Nano-Formulation in LPS-Induced Acute Kidney Injury in Mice: Evaluation of Oxidative Stress, Biochemical & Molecular Parameters

ALRashdi,

Massoud,

Rashwan

et al. 2024

JIR

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Background Acute kidney injury (AKI) is a medical concern that is accompanied by the rapid deterioration of kidney function. It can be triggered by lipopolysaccharide (LPS) of gram-negative bacteria as it activates a complicated immune response, resulting in widespread inflammation and potential organ dysfunction. Black seed oil (BSO) is rich in beneficial constituents and has been widely used owing to its nutritional advantages. Purpose This research is aimed to investigate the potential protective effects of BSO and its nano-formulation on AKI induced by LPS. It also aimed to compare their anti-inflammatory activity with indomethacin, a known synthetic anti-inflammatory drug. Materials and Methods Forty-eight mice were placed randomly into 8 groups. A single intraperitoneal ( i.p .) injection of 2.5 mg/kg B.W. of LPS was used to trigger inflammation, and pretreatment with BSO and its nano-formulation was at 0.2 mL/kg/day for 14 consecutive days. Indomethacin was used as a reference drug and its efficacy was tested alone or in combination with BSO at lower doses. Renal function was assessed using urea, creatinine, neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1). Also, oxidative and inflammatory markers were assessed by measuring levels of reduced glutathione (GSH), nitric oxide (NO), cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), and toll-like receptor-4 (TLR-4). Histopathological examination of the kidney tissues was also performed. Results The study showed that BSO and its nano-formulation had anti-inflammatory effects comparable to or better than those of indomethacin. They greatly decreased the oxidative stress and inflammatory markers induced by LPS. Their protective effect against pathological alterations in kidney tissues was significantly noticed. Conclusion BSO and its nano-formulation could be used as nephroprotective and anti-inflammatory supplements.

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Antioxidant and anti-inflammatory potential of spirulina and thymoquinone mitigate the methotrexate-induced neurotoxicity

Cited by 11 publications

References 66 publications

The potential neuroprotective of luteolin against acetamiprid-induced neurotoxicity in the rat cerebral cortex

The potential neuroprotective of luteolin against acetamiprid-induced neurotoxicity in the rat cerebral cortex

Investigation of the effects of curcumin and piperine on cyclophosphamide‐induced brain injury in rats

The Protecting Role of Black Seed Oil and Its Nano-Formulation in LPS-Induced Acute Kidney Injury in Mice: Evaluation of Oxidative Stress, Biochemical & Molecular Parameters

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