Antioxidant and anti‐inflammatory properties of tomato fruits synthesizing different amounts of stilbenes

Abstract

SummaryResveratrol, a plant phenolic compound, is found in grapes and red wine, but is not widely distributed in other common food sources. The pathway for resveratrol biosynthesis is well characterized. Metabolic engineering of this compound has been achieved in tomato plants capability and ascorbate content in transformed fruits were also evaluated, and a significant increase in both was found in the LoxS and 35SS lines. These results could explain the higher capability of transgenic fruits to counteract the pro-inflammatory effects of phorbol ester in monocyte-macrophages via the inhibition of induced cyclo-oxygenase-2 enzyme.

Administration of Reconstituted Polyphenol Oil Bodies Efficiently Suppresses Dendritic Cell Inflammatory Pathways and Acute Intestinal Inflammation

“…After centrifugation at 1000 g for 5 minutes, the methyl-butyl-ether phase was recovered, dried (rotavapor), and resuspended into 50% ethanol. Quercetin was quantified by reverse phase-HPLC using the method described above [41] onto a 1100 Agilent work chromatographic station equipped with a photodiodes array UV-Vis detection system. The elution profile was monitored at 370 nm.…”

Elicitors: A Tool for Improving Fruit Phenolic Content

“…Tomato plants synthesizing resveratrol were obtained via the heterologous expression of a grape (Vitis vinifera L.) cDNA encoding for the enzyme stilbene synthase. The transgenic plants accumulated trans-resveratrol and trans-piceid in their skin and had a higher antioxidant capacity and ascorbate content than the wild tomato [41]. In addition, metabolic engineering of the flavonoid biosynthesis pathways in apple was performed by overexpressing the maize leaf color regulatory gene in the mentioned fruit.…”

Plant NADPH-cytochrome P450 oxidoreductases

“…Accordingly P450-catalysed reactions are integrated steps in the formation of many of these compounds and optimization of P450 enzyme activities a key target in yield improvement efforts to render such approaches economically feasible. Numerous examples of successful formation of new desired products by introduction of P450s into plants using genetic engineering are available (blue roses, resveratrol, dhurrin) (D'Introno et al, 2009;Delaunois et al, 2009;Katsumoto et al, 2007;Tattersall et al, 2001). One potential problem associated with the introduction of plant P450s into a new host such as other plant species, yeasts or bacteria is to ensure an adequate supply of electrons from CPR to optimize P450 activity.…”