Antioxidant and anti-inflammatory properties of cancer preventive peptide lunasin in RAW 264.7 macrophages

Hernández-Ledesma, Blanca; Hsieh, Chia Chien; Lumen, Ben O. de

doi:10.1016/j.bbrc.2009.10.053

Cited by 217 publications

(137 citation statements)

References 30 publications

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“…Pro-inflammatory cytokines IL-1β, IL-6, and TNF-α, which are mainly produced by activated monocytes or macrophages, stimulate bone resorption and also enhance the production of PGE 2 in several types of cells (Hernández-Ledesma et al 2009). It is well known that a high concentration of pro-inflammatory cytokines plays a critical role in the induction of iNOS through activation of nuclear factor-κB.…”

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confidence: 99%

5β-Hydroxypalisadin B isolated from red alga Laurencia snackeyi attenuates inflammatory response in lipopolysaccharide-stimulated RAW 264.7 macrophages

Wijesinghe¹,

Kang²,

Lee³

et al. 2014

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In this study, four compounds isolated from the red alga Laurencia snackeyi were evaluated for their potential antiinflammatory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. These compounds were tested for their inhibitory effects on nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. Since 5β-hydroxypalisadin B showed the best activity it was further tested for the production of prostaglandin-E 2 (PGE 2 ), expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), the release of pro-inflammatory cytokines tumor necrotic factor-alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). 5β-Hydroxypalisadin B significantly reduced the PGE 2 release and suppressed the iNOS and COX-2 expression in LPS-stimulated RAW 264.7 cells. It also significantly reduced the release of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6. These findings provide the first evidence of antiinflammatory potential of 5β-hydroxypalisadin B isolated from the red alga L. snackeyi and hence, it could be exploited as an active ingredient in pharmaceutical, nutraceutical and functional food applications.

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mentioning

confidence: 99%

5β-Hydroxypalisadin B isolated from red alga Laurencia snackeyi attenuates inflammatory response in lipopolysaccharide-stimulated RAW 264.7 macrophages

Wijesinghe¹,

Kang²,

Lee³

et al. 2014

ALGAE

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“…ii) Lunasin was also able to induce the inducible nitric oxide synthase/nitric oxide signaling pathway through adjustment of the cyclo-oxygenase 2/PGE 2 ratio (59). iii) Lunasin was able to perform its antioxidant function through decreasing LPS-induced reactive oxygen species production (57), and prevent the oxidative damage of DNA by inhibiting the generation of hydroxyl free radicals (60). The anti-inflammatory and antioxidant functions of lunasin improve its potential use in the prevention and treatment of cancer.…”

Section: Molecular Mechanisms Of Lunasin In the Prevention And Treatmmentioning

confidence: 99%

Lunasin: A promising polypeptide for the prevention and treatment of cancer

et al. 2017

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Abstract. Strategies for the treatment of cancer remain unsatisfactory due to the poor understanding of the complicated underlying molecular mechanisms of carcinogenesis. A number of types of cancer exhibit a marked association with dietary habits and lifestyles. Therefore, the modulation of dietary habits or lifestyles may be an effective strategy for preventing the formation and progression of cancer. Proteins and polypeptides from soybean have been developed as healthcare products due to their marked activity in inhibiting the progression of cancer at various stages. Lunasin, containing 43 amino acid residues, is one such example of a soybean-derived polypeptide that has been demonstrated to exhibit marked anti-cancer activity. In the present review, studies of the underlying molecular mechanisms and potential advantages of lunasin in the prevention and treatment of cancer have been examined, to provide a theoretical reference for the development of natural product-based agents or healthcare products for the prevention and treatment of cancer. IntroductionCancer is well-known to be one of the leading causes of mortality worldwide (1). However, the therapy of various types of cancer remains unsatisfactory due to the poor understanding of the complicated underlying molecular mechanisms of carcinogenesis. A number of epidemiological studies have demonstrated that a number of types of cancer exhibit a marked association with dietary habits and lifestyles (2-4). A statistical study has demonstrated that ~3.07 million individuals in China were diagnosed with cancer in 2012, which accounts for 1/5 of the total number of patients with cancer worldwide (5). Furthermore, ~2.21 million patients succumbed to cancer in China, which is ~25% of the total mortality worldwide due to cancer (5). Epidemiological evidence has demonstrated that cancer in 35% of patients exhibits a marked association with lifestyle, particularly diet (2). Therefore, the adjustment of dietary habits and lifestyles may be an effective strategy to prevent carcinogenesis. Cancer cell-and tumor-bearing animal models have demonstrated that the consumption of foods containing natural compounds with anti-cancer activity is able to markedly reduce the risk of cancer, and increase the sensitivity of cancer cells to treatment (6). A number of phytochemicals, including resveratrol, quercetin and flavonoids, have been demonstrated to exhibit marked anti-cancer activity (7-9). Similarly, foodborne proteins and polypeptides have attracted attention due to their specific advantages as anti-cancer substances (10,11). Compared with certain small-molecule drugs, polypeptides exhibit characteristics of increased affinity, marked ability of specific targeting and decreased toxicity; furthermore, polypeptides exhibit increased permeability in tissues compared with protein-based drugs (12). Therefore, polypeptides have been recognized as potential natural anti-cancer substances for inhibiting the development and progression of cancer at different stages (13).Following ...

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“…Lunasin has been found to exert anti-inflammatory activity that might contribute to its chemopreventive properties. First studies demonstrated that lunasin potently inhibits lipopolysaccharide (LPS)-induced production of pro-inflammatory mediators interleuquine-6 (IL-6), tumor necrosis factor-α (TNF-α), and prostaglandin (PG) E2 (PGE2) in macrophage RAW 264.7 cells [69], through modulation of cyclooxygenase-2 (COX-2)/PGE2 and inducible nitric oxide synthase/nitric oxide pathways, and suppression of NF-B pathways [70,71]. Larkins and co-workers (2006) have demonstrated that COX-2 inhibition can decrease breast cancer cells motility, invasion and matrix metalloproteinase expression [72].…”

Section: Anti-inflammatory and Antioxidant Activities Of Lunasinmentioning

confidence: 99%