Antioxidant and anti-matrix metalloproteinases activities of dried longan (Euphoria longana) seed extract

Abstract

Longan, a major fruit crop in Thailand, is a rich source of antioxidant polyphenic compounds, mostly gallic acid and ellagic acid. Dried longan seeds contain large amounts of antioxidant polyphenols. In this study, three fractions of dried longan seed extract were collected by Sephadex LH-20 column chromatography to determine the major compounds in each, and the amounts of gallic acid and ellagic acid were quantified. The first fraction was determined to be the major source of gallic acid, while ellagic acid was mainly found in fraction 3. Antioxidant activities were measured using total phenolic content, total flavonoid content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and metal chelating activity. Fraction 1 had the highest antioxidant activity. In addition, the effect of each fraction on matrix metalloproteinases (MMPs) activity was analysed using a fluorometric assay. Interestingly, fraction 3 had the greatest MMPs inhibitory effect. Notably, dried longan seeds have been established not only as major sources of antioxidants, but also as potent MMPs inhibitors. Since these properties are associated with anti-cancer effects, dried longan seeds could be a novel natural source for compounds used in chemoprevention and chemotherapy.

In vitro Biological Activities of the Anti-aging Potential of Dimocarpus longan Leaf Extracts

“…Although D. longan leaves were reported to contain gallic acid, but the other active compounds for anti-aging in the leaf might be more potent than gallic acid due to the activities themselves or synergistic effects of other phenolic or active compounds consisting in the extracts. While inhibition of collagenase activity of D. longan leaf extract has not been reported to date, Panyathep et al (2013) revealed the inhibition of MMP-13 of D. longan seed extracts. The gallic acid-enriched and ellagic acid-enriched seed fraction seemed to have more inhibitory potency than gallic acid or ellagic acid.…”

Antioxidant and in vitro anticancer activities of phenolics isolated from sugar beet molasses

“…The three compounds, GA, CGC and EP, obtained in our study are three typical phenolic compounds in plants which have been reported to possess strong antioxidant activity [ 26 , 27 ]. According to reports, free hydroxyl groups in phenolics are mainly responsible for antioxidant activity [ 28 , 29 ].…”

Utilization of Plant-Based Agricultural Waste by Subcritical Water Treatment

“…Thailand [2]. The utilization of fruit waste by processing it into dietary fiber, which can be used as a food ingredient or diet supplement, has also been extensively studied [3].…”