Antioxidant and antiapoptotic activities of Calotropis procera latex on Catfish (Clarias gariepinus) exposed to toxic 4-nonylphenol

Sayed, Alaa El‐Din H.; Mohamed, Nadia H.; Ismail, M.A.; Abdel‐Mageed, Wael M.; Shoreit, A. A. M.

doi:10.1016/j.ecoenv.2016.02.023

Cited by 44 publications

(23 citation statements)

References 35 publications

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“…The observed differences between medaka and catfish may be related to the characteristics of their different evolutionary backgrounds . The present study demonstrated that UVA caused DNA damage in African catfish, as detected by a comet assay, similar results were reported in this species after 4‐NP exposure , and in medaka after UVA exposure . The findings also revealed differences between medaka and catfish in terms of genotoxicity responses (apoptosis and erythrocytes alterations).…”

Section: Discussionsupporting

confidence: 84%

UVA‐Induced DNA Damage and Apoptosis in Red Blood Cells of the African Catfish Clarias gariepinus

Sayed

2017

Photochem & Photobiology

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Ultraviolet-A light (UVA)-induced DNA damage and repair in red blood cells to investigate the sensitivity of African catfish to UVA exposure is reported. Fishes were irradiated with various doses of UVA light (15, 30, and 60 min day À1 for 3 days). Morphological and nuclear abnormalities in red blood cells were observed in the fish exposed to UVA compared with controls. Morphological alterations such as acanthocytes, crenated cells, swollen cells, teardrop-like cells, hemolyzed cells, and sickle cells were observed. Those alterations were increased after 24 h exposure to UVA light and decreased at 14 days after exposure. The percentage of apoptosis was higher in red blood cells exposed to higher doses of UVA light. No micronuclei were detected, but small nuclear abnormalities such as deformed and eccentric nuclei were observed in some groups. We concluded that exposure to UVA light induced DNA damage, apoptosis, and morphological alterations in red blood cells in catfish; however, catfish were found to be less sensitive to UVA light than wild-type medaka.

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Section: Discussionsupporting

confidence: 84%

UVA‐Induced DNA Damage and Apoptosis in Red Blood Cells of the African Catfish Clarias gariepinus

Sayed

2017

Photochem & Photobiology

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“…Also, values of I z in T L were significantly higher (p<0.001; 2.3 times) than the value of C z (101.8±2.4 μm), and a similar outcome between C z and I z was found in T EM (51.7±2.0; 2.9 times). Numerous research on comet bioassays in polluted streams reported that the main causes of DNA damage in eukaryotic cells of aquatic organisms are closely associated with heavy metals, polyaromatic hydrocarbon (PAHs), agricultural chemicals (pesticides, herbicides) and genotoxicants [55][56][57][58][59].…”

Section: Dna Damage Assessmentmentioning

confidence: 99%

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Bae¹,

Atique²,

Yoon³

et al. 2020

Pol. J. Environ. Stud.

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Ecological risk assessment was carried out in two urban streams, viz. Gap Stream (GS) and Miho Stream (MS), in the Geum River Watershed during July 2013-October 2014. The techniques used in this study included multi-level fish biomarkers of DNA damage based on single-cell gel electrophoresis (SCGE) coupled with the study of physiological responses based on 7-ethoxyresorufin-O-deethylase (EROD) and acetylcholinesterase (AChE) activities in fish species. Biomarker values of tail DNA (tDNA), tail length (T L), and tail extent moment (TEM) in impacted zone (I z) were 2.0-3.6-fold greater than in controls (C z). Nucleus image analysis showed that the nucleus had circular particle forms in the C z as compared with a longitudinal oval form with broken particles from the nucleus in the I z. Physiological response analysis of EROD and AchE activities indicated that their levels were higher in the I z than in the C z. Such DNA damages and greater physiological responses in the I z were attributed to chemical contaminants discharged from the wastewater disposal plants and industrial complex. This combination of DNA damage and physiological responses approach can be used as a key tool for early-warning detection of chemical contaminants and concomitant risks to the ecological health of urban streams.

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“…When the organism is treated with toxic chemical, superoxidase dismutase, catalase, acetlycholinstrase (AchE), glutathione s-transferase and cortisol, G6PDH) exhibited a significant increase in their activity (P<0.05). However, when the tested organism was treated with the chemical pollutant (4-nonylphenol) plus the latex of Calotropis procera, the activity of those enzymes decreased, which indicate an antioxidant potential 45 .…”

Section: Calotropis Proceramentioning

confidence: 99%

“…On the other hand, the root extract showed the lowest Inhibitory Concentration (IC50 = 0.27 mg/ ml) 8 . A recent study reported the antioxidant and antiapoptotic properties of Calotropis procera latex on Clarias gariepinus that is treated with the chemical pollutant 4-nonylphenol 45 . When the organism is treated with toxic chemical, superoxidase dismutase, catalase, acetlycholinstrase (AchE), glutathione s-transferase and cortisol, G6PDH) exhibited a significant increase in their activity (P<0.05).…”

Section: Calotropis Proceramentioning

confidence: 99%

Ethnomedicine, phytochemistry and pharmacology of Calotropis procera and Tribulus terrestris

Dirir

Cheruth²,

Ksiksi³

2017

Journal of Natural Remedies

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Antioxidant and antiapoptotic activities of Calotropis procera latex on Catfish (Clarias gariepinus) exposed to toxic 4-nonylphenol

Cited by 44 publications

References 35 publications

UVA‐Induced DNA Damage and Apoptosis in Red Blood Cells of the African Catfish Clarias gariepinus

UVA‐Induced DNA Damage and Apoptosis in Red Blood Cells of the African Catfish Clarias gariepinus

Ecological Risk Assessment of Urban Streams Using Fish Biomarkers of DNA Damage and Physiological Responses

Ethnomedicine, phytochemistry and pharmacology of Calotropis procera and Tribulus terrestris

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